{
  "schemaVersion": "1.2",
  "reviewedAt": "2026-08-12",
  "publisher": "Molt & Moss",
  "reviewStatus": "No independent review claimed",
  "responsePath": "/research/challenge",
  "lenses": [
    {
      "id": "taxonomy",
      "label": "Taxonomy and identity",
      "qualification": "Relevant atyid taxonomy, morphology, nomenclature, population genetics or species identification experience.",
      "focus": "Check whether taxon names, identity assumptions, trade-line language and transfers across populations remain explicit."
    },
    {
      "id": "design",
      "label": "Experimental design and statistics",
      "qualification": "Experience evaluating biological experimental units, replication, allocation, denominators, endpoints and statistical inference.",
      "focus": "Check whether animal counts are confused with independent units, endpoints or comparisons are overstated, and uncertainty is preserved."
    },
    {
      "id": "husbandry",
      "label": "Aquatic husbandry and welfare",
      "qualification": "Documented freshwater crustacean, aquatic veterinary, laboratory-animal or evidence-based aquarium husbandry experience.",
      "focus": "Check whether actions are measurable, reversible where possible, welfare-conscious and separated from diagnosis or treatment authorization."
    },
    {
      "id": "synthesis",
      "label": "Evidence synthesis and science communication",
      "qualification": "Experience in systematic review, evidence appraisal, research integrity, scientific editing or bounded public communication.",
      "focus": "Check source roles, support-to-limit logic, omitted contrary evidence, accessibility and whether the public wording outruns the source."
    }
  ],
  "disclosure": [
    "Molt & Moss is a one-person seller-publisher that intends to sell shrimp and supplies.",
    "AI tools assisted research organization, drafting, software and consistency checks; they are not sources or reviewers.",
    "No independent peer review of this packet is currently claimed.",
    "Reviewers are asked to disclose relevant seller, breeder, author, employer, affiliate, paid or gifted relationships."
  ],
  "responseInstructions": [
    "Submit one exact claim and one stable source per evidence challenge.",
    "Begin the source summary with the review lens and the relevant experience used for this critique.",
    "State any relevant seller, breeder, author, employer, affiliate, paid or gifted relationship, or write none.",
    "Propose exact replacement wording and keep it inside the source methods and results.",
    "Molt & Moss will publish a finite outcome for substantive completed reviews without treating reviewer status as proof by itself."
  ],
  "reviewQuestions": [
    "Is the reviewed claim stated precisely enough to audit?",
    "Does the supported wording stay inside the source taxon, unit, comparison, duration and endpoint?",
    "Does the limitation name the nearest consequential inference the evidence cannot carry?",
    "Is the next action observable, measurable or reversible without becoming diagnosis or treatment authorization?",
    "Is a consequential source, contrary result, conflict or uncertainty missing?",
    "What exact wording should change, if any, and which stable source supports that change?"
  ],
  "evidenceComparisons": [
    {
      "id": "temperature-outcomes",
      "title": "Temperature results depend on the outcome being measured",
      "path": "/research/disagreements/temperature-outcomes",
      "whyItLooksConflicting": "Shorter incubation, altered growth, delayed maturation, suppressed reproduction and changed sex allocation can all be reported as a temperature effect, even though they are not the same outcome.",
      "reconciliation": "The studies can coexist because they used different temperature treatments, durations, life stages and endpoints. A faster process is not automatically a better lifetime outcome, and survival during one protocol does not make every tested temperature equivalent.",
      "unresolved": "The corpus does not establish one universal aquarium optimum, a lifetime safe interval or how commercial lines differ across complete life cycles.",
      "sourceRecordIds": [
        "tropea-2015-temperature",
        "balina-2018-high-temperature",
        "tomas-2021-temperature",
        "serezli-2017-temperature-sex-ratio"
      ]
    },
    {
      "id": "grazing-and-system-nutrients",
      "title": "Grazing on algae is not the same as cleaning an aquarium",
      "path": "/research/disagreements/grazing-and-system-nutrients",
      "whyItLooksConflicting": "A reader may treat fewer algal cells, lower leaf-periphyton chlorophyll, faster litter breakdown and higher water-column nutrients as competing verdicts on whether shrimp make a system clean.",
      "reconciliation": "The studies measured different compartments and processes. Consumption or disturbance can reduce algae on a surface while recycling material into feces, dissolved nutrients or suspended production. Local removal is not system export.",
      "unresolved": "The corpus does not provide a home-aquarium cleaning rate, a stocking rule for algae control or a nutrient budget that transfers across tanks.",
      "sourceRecordIds": [
        "groendahl-2017-algal-grazing",
        "ye-2019-macrophyte-periphyton",
        "kong-2019-cattail-decomposition"
      ]
    },
    {
      "id": "colour-phenotype-and-identity",
      "title": "Visible colour can change without proving inherited identity",
      "path": "/research/disagreements/colour-phenotype-and-identity",
      "whyItLooksConflicting": "Background and diet studies show measurable appearance changes, while molecular studies identify pigmentation pathways and market labels imply inherited lines.",
      "reconciliation": "Environmental plasticity and biological pigmentation mechanisms can both be real. A visible phenotype reflects genes, development, environment, measurement and selection history; changing one visible measure does not authenticate ancestry or genetic purity.",
      "unresolved": "The corpus cannot map every named trade line to a validated genotype, predict offspring from seller labels or separate all environmental and inherited contributions from a photograph.",
      "sourceRecordIds": [
        "tomas-2020-background",
        "ishiba-2025-environment-colour",
        "tomas-2020-diets",
        "zhang-2025-scarb1-pigmentation",
        "kusmintarsih-2023-market-co1"
      ]
    },
    {
      "id": "feeding-outcomes",
      "title": "A diet result is specific to its formulation and endpoint",
      "path": "/research/disagreements/feeding-outcomes",
      "whyItLooksConflicting": "Studies report colour, growth, female reproduction, offspring quality or short-term survival under different feeds, and each positive result can be retold as proof of a universally superior food.",
      "reconciliation": "The experiments compare different formulations, controls, rations, life stages and outcome windows. A food that changes one pigment measure is not therefore complete, and a reproductive result cannot be assumed for juveniles or every commercial product.",
      "unresolved": "The corpus does not establish one complete diet, universal portion, feeding frequency or long-term product ranking across lines and colony conditions.",
      "sourceRecordIds": [
        "tomas-2020-diets",
        "sganga-2020-commercial-diets",
        "luna-vivaldo-2024-live-microalgae-colour",
        "wijaya-2023-fermented-vegetable-feed"
      ]
    },
    {
      "id": "chemical-response-and-safe-threshold",
      "title": "A biological response is not automatically a care threshold",
      "path": "/research/disagreements/chemical-response-and-safe-threshold",
      "whyItLooksConflicting": "Mortality, enzyme activity and transcript abundance may change at different concentrations and times, tempting readers to interpret the lowest observed response or a no-death result as a universal safety line.",
      "reconciliation": "These endpoints answer different questions. Molecular or biochemical responses can occur without measured mortality, while a short no-death observation does not establish chronic safety. Nominal exposure, water chemistry and taxon verification also affect transferability.",
      "unresolved": "The corpus does not establish a universal home-aquarium copper threshold, product-dose rule, antidote or chronic no-effect level for every water chemistry and line.",
      "sourceRecordIds": [
        "li-2015-copper-chlorpyrifos",
        "kim-2018-copper-transcripts"
      ]
    },
    {
      "id": "taxonomy-name-history",
      "title": "A published species name is evidence context, not permanent identity",
      "path": "/research/disagreements/taxonomy-name-history",
      "whyItLooksConflicting": "Older field records, morphology-based revisions, mitochondrial barcodes and integrative taxonomic studies may place similar-looking material under different names or lineages.",
      "reconciliation": "Taxonomic conclusions depend on the specimens, characters, loci, reference material and species concept used. Later reassignment changes how an older result should be scoped; it does not erase the observation or transfer it automatically to N. davidi.",
      "unresolved": "The corpus does not resolve every Neocaridina species treatment, authenticate commercial animals from a photograph or make one barcode a universal identity test.",
      "sourceRecordIds": [
        "cai-ng-1999-serrata-revision",
        "shih-2024-japan-neocaridina",
        "kusmintarsih-2023-market-co1",
        "zhou-2021-henan-shrimp"
      ]
    }
  ],
  "items": [
    {
      "id": "cycling-time",
      "claim": "A tank is cycled after a fixed number of weeks",
      "supported": "microbial nitrogen processing can become established in aquarium systems.",
      "notEstablished": "age, nitrate, clear water or a bottled product alone does not prove that one tank can process its intended load.",
      "nextAction": "test a known fishless challenge and preserve total-ammonia and nitrite reporting bases.",
      "claimPath": "/care/claims/cycling-time",
      "guidePath": "/care/cycling",
      "dossierPath": "/research/guides/cycling",
      "evidence": [
        {
          "id": "hovanec-1998-nitrospira",
          "role": "interpretive-context",
          "use": "Aquarium biofilters can contain ammonia- and nitrite-oxidizing communities that differ from older assumptions.",
          "path": "/research/records/hovanec-1998-nitrospira",
          "sourceIntegrityState": "crossref-query-unmatched",
          "sourceIntegrityLabel": "Canonical DOI outside query result",
          "sourceIntegrityBoundary": "The record has a canonical DOI but did not appear in the broad Neocaridina query. It requires DOI-specific or alternative-registry status review before any no-update statement."
        },
        {
          "id": "sauder-2011-aquarium-nitrification",
          "role": "interpretive-context",
          "use": "Aquarium nitrification is a measurable microbial process rather than a calendar label.",
          "path": "/research/records/sauder-2011-aquarium-nitrification",
          "sourceIntegrityState": "crossref-query-unmatched",
          "sourceIntegrityLabel": "Canonical DOI outside query result",
          "sourceIntegrityBoundary": "The record has a canonical DOI but did not appear in the broad Neocaridina query. It requires DOI-specific or alternative-registry status review before any no-update statement."
        },
        {
          "id": "scagnelli-2023-quick-start",
          "role": "limitation-context",
          "use": "A bacterial-start product study does not prove that any product, tank age or appearance establishes intended-load processing.",
          "path": "/research/records/scagnelli-2023-quick-start",
          "sourceIntegrityState": "crossref-query-unmatched",
          "sourceIntegrityLabel": "Canonical DOI outside query result",
          "sourceIntegrityBoundary": "The record has a canonical DOI but did not appear in the broad Neocaridina query. It requires DOI-specific or alternative-registry status review before any no-update statement."
        }
      ],
      "authorities": [
        {
          "id": "epa-method-detection-limit",
          "use": "Keeps not-detected wording tied to the registered method rather than absolute zero.",
          "path": "/research/authorities/epa-method-detection-limit"
        }
      ],
      "gaps": [
        {
          "id": "chronic-ammonia-nitrite",
          "boundary": "A direct N. davidi thesis provides a 96-hour acute juvenile experiment. An official abstract for a restricted 2007 N. denticulata thesis reports chronic total-ammonia growth and reproductive comparisons, but its complete methods and results are unavailable. Neither source establishes a chronic operating range, and no controlled chronic nitrite study was located.",
          "path": "/research/gaps/chronic-ammonia-nitrite"
        },
        {
          "id": "filter-turnover",
          "boundary": "A field survey associated lower current velocity with higher juvenile density, but no assigned aquarium comparison supports a universal turnovers-per-hour rule or one required filter design.",
          "path": "/research/gaps/filter-turnover"
        }
      ],
      "comparisons": []
    },
    {
      "id": "tds-number",
      "claim": "A TDS number tells you whether the minerals are right",
      "supported": "a meter can estimate TDS from conductivity using its conversion factor.",
      "notEstablished": "the display does not reveal ionic composition, separate calcium and magnesium, GH, contaminants or a species optimum.",
      "nextAction": "record meter mode and temperature basis, then measure the property the decision requires.",
      "claimPath": "/care/claims/tds-number",
      "guidePath": "/care/parameters",
      "dossierPath": "/research/guides/parameters",
      "evidence": [
        {
          "id": "naigaga-2017-water-test-kits",
          "role": "limitation-context",
          "use": "Aquarium measurement tools require method-specific interpretation and cannot reveal properties they do not measure.",
          "path": "/research/records/naigaga-2017-water-test-kits",
          "sourceIntegrityState": "crossref-query-unmatched",
          "sourceIntegrityLabel": "Canonical DOI outside query result",
          "sourceIntegrityBoundary": "The record has a canonical DOI but did not appear in the broad Neocaridina query. It requires DOI-specific or alternative-registry status review before any no-update statement."
        }
      ],
      "authorities": [
        {
          "id": "usgs-specific-conductance",
          "use": "Defines conductance measurement and reporting context.",
          "path": "/research/authorities/usgs-specific-conductance"
        },
        {
          "id": "nist-conductivity-meter-manual",
          "use": "Shows that TDS display is derived through an instrument conversion factor.",
          "path": "/research/authorities/nist-conductivity-meter-manual"
        },
        {
          "id": "usgs-hardness",
          "use": "Separates hardness from a conductivity-derived TDS estimate.",
          "path": "/research/authorities/usgs-hardness"
        }
      ],
      "gaps": [
        {
          "id": "water-parameter-optima",
          "boundary": "Field occurrences and study culture water describe places or reproducible conditions, not species-wide GH, KH, pH or conductivity optima.",
          "path": "/research/gaps/water-parameter-optima"
        }
      ],
      "comparisons": []
    },
    {
      "id": "water-change-percent",
      "claim": "Large water changes kill shrimp",
      "supported": "a change can expose animals to differences in temperature, dissolved composition, pH, contaminants and gas state.",
      "notEstablished": "percentage alone does not determine safety, and a tiny change is not automatically useful or harmless.",
      "nextAction": "identify the purpose, compare source and tank water, calculate dilution and observe the result.",
      "claimPath": "/care/claims/water-change-percent",
      "guidePath": "/care/water-changes",
      "dossierPath": "/research/guides/water-changes",
      "evidence": [
        {
          "id": "viau-2016-zero-exchange",
          "role": "limitation-context",
          "use": "One defined zero-exchange culture system does not establish a universal water-change percentage.",
          "path": "/research/records/viau-2016-zero-exchange",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        },
        {
          "id": "melo-2023-nitrogen",
          "role": "limitation-context",
          "use": "An acute nitrogen experiment cannot make water-change percentage the sole safety variable.",
          "path": "/research/records/melo-2023-nitrogen",
          "sourceIntegrityState": "no-canonical-doi",
          "sourceIntegrityLabel": "No canonical DOI in registry",
          "sourceIntegrityBoundary": "The evidence record uses another stable source URL. Crossref DOI update metadata cannot audit it."
        }
      ],
      "authorities": [
        {
          "id": "usgs-water-quality-primer",
          "use": "Supports dissolved-material and evaporation context for replacement water.",
          "path": "/research/authorities/usgs-water-quality-primer"
        },
        {
          "id": "cdc-chlorine-chloramine",
          "use": "Separates source-water disinfectant context from percentage alone.",
          "path": "/research/authorities/cdc-chlorine-chloramine"
        }
      ],
      "gaps": [
        {
          "id": "chronic-nitrate",
          "boundary": "No controlled chronic nitrate-only Neocaridina experiment was located in the current review.",
          "path": "/research/gaps/chronic-nitrate"
        },
        {
          "id": "chronic-ammonia-nitrite",
          "boundary": "A direct N. davidi thesis provides a 96-hour acute juvenile experiment. An official abstract for a restricted 2007 N. denticulata thesis reports chronic total-ammonia growth and reproductive comparisons, but its complete methods and results are unavailable. Neither source establishes a chronic operating range, and no controlled chronic nitrite study was located.",
          "path": "/research/gaps/chronic-ammonia-nitrite"
        },
        {
          "id": "water-parameter-optima",
          "boundary": "Field occurrences and study culture water describe places or reproducible conditions, not species-wide GH, KH, pH or conductivity optima.",
          "path": "/research/gaps/water-parameter-optima"
        }
      ],
      "comparisons": []
    },
    {
      "id": "molt-calcium",
      "claim": "A failed molt means the shrimp needs more calcium",
      "supported": "mineral availability and water chemistry matter to exoskeleton biology.",
      "notEstablished": "one incomplete molt identifies a calcium deficiency, proves low GH caused it or validates adding a mineral product.",
      "nextAction": "distinguish the animal from an exuvia, measure immediate water hazards and preserve the event history.",
      "claimPath": "/care/claims/molt-calcium",
      "guidePath": "/care/molting",
      "dossierPath": "/research/guides/molting",
      "evidence": [
        {
          "id": "gao-2024-cuticle-calcium-proteins",
          "role": "interpretive-context",
          "use": "Calcium-associated cuticular proteins are involved in exoskeleton biology under the studied molecular methods.",
          "path": "/research/records/gao-2024-cuticle-calcium-proteins",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        },
        {
          "id": "greenaway-1985-calcium-review",
          "role": "interpretive-context",
          "use": "Crustacean calcium balance is biologically relevant but does not diagnose one aquarium molt event.",
          "path": "/research/records/greenaway-1985-calcium-review",
          "sourceIntegrityState": "crossref-query-unmatched",
          "sourceIntegrityLabel": "Canonical DOI outside query result",
          "sourceIntegrityBoundary": "The record has a canonical DOI but did not appear in the broad Neocaridina query. It requires DOI-specific or alternative-registry status review before any no-update statement."
        },
        {
          "id": "hu-2019-insecticides",
          "role": "limitation-context",
          "use": "Molt-related outcomes can occur in a chemical-exposure context, preventing a one-cause calcium inference.",
          "path": "/research/records/hu-2019-insecticides",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        }
      ],
      "authorities": [
        {
          "id": "usgs-hardness",
          "use": "Defines hardness while explicitly refusing a molt diagnosis or species optimum.",
          "path": "/research/authorities/usgs-hardness"
        }
      ],
      "gaps": [
        {
          "id": "water-parameter-optima",
          "boundary": "Field occurrences and study culture water describe places or reproducible conditions, not species-wide GH, KH, pH or conductivity optima.",
          "path": "/research/gaps/water-parameter-optima"
        }
      ],
      "comparisons": []
    },
    {
      "id": "cleanup-crew",
      "claim": "Neocaridina clean the tank",
      "supported": "Neocaridina graze algae, microbes, detrital material and food-associated surfaces.",
      "notEstablished": "grazing guarantees control of a visible growth, replaces maintenance or exports the consumed nutrients from the aquarium.",
      "nextAction": "identify the material and correct light, inputs or maintenance while treating shrimp as animals, not equipment.",
      "claimPath": "/care/claims/cleanup-crew",
      "guidePath": "/care/algae",
      "dossierPath": "/research/guides/algae",
      "evidence": [
        {
          "id": "groendahl-2017-algal-grazing",
          "role": "direct-support",
          "use": "Directly measured grazing-related outcomes under its stated comparison.",
          "path": "/research/records/groendahl-2017-algal-grazing",
          "sourceIntegrityState": "crossref-query-unmatched",
          "sourceIntegrityLabel": "Canonical DOI outside query result",
          "sourceIntegrityBoundary": "The record has a canonical DOI but did not appear in the broad Neocaridina query. It requires DOI-specific or alternative-registry status review before any no-update statement."
        },
        {
          "id": "ye-2019-macrophyte-periphyton",
          "role": "direct-support",
          "use": "Separates local periphyton and plant outcomes from water-column nutrient and chlorophyll outcomes.",
          "path": "/research/records/ye-2019-macrophyte-periphyton",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        },
        {
          "id": "kong-2019-cattail-decomposition",
          "role": "limitation-context",
          "use": "Separates litter breakdown and fecal effects from proven intake or whole-tank cleaning.",
          "path": "/research/records/kong-2019-cattail-decomposition",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        }
      ],
      "authorities": [],
      "gaps": [
        {
          "id": "botanical-litter-outcomes",
          "boundary": "One abstract-level N. davidi study reports leaf choices and normalized consumption. A fully reviewed N. denticulata microcosm study separates direct shrimp contact from fecal contact on one cattail preparation and measures litter breakdown, enzymes and water chemistry, but not intake, dietary adequacy or long-term shrimp outcomes. The taxa, litter species and endpoints differ, and neither record establishes disease benefit or a replacement interval.",
          "path": "/research/gaps/botanical-litter-outcomes"
        }
      ],
      "comparisons": [
        {
          "id": "grazing-and-system-nutrients",
          "title": "Grazing on algae is not the same as cleaning an aquarium",
          "path": "/research/disagreements/grazing-and-system-nutrients"
        }
      ]
    },
    {
      "id": "peaceful-fish",
      "claim": "Peaceful fish are shrimp-safe",
      "supported": "outcomes depend on the exact fish species, individual size, shrimp life stage, cover, feeding, intake protection and the tank's goal.",
      "notEstablished": "a retailer temperament label predicts juvenile retention or preserves a breeding line.",
      "nextAction": "decide whether the goal is adult display or colony recruitment, then evaluate the exact species.",
      "claimPath": "/care/claims/peaceful-fish",
      "guidePath": "/care/tankmates",
      "dossierPath": "/research/guides/tankmates",
      "evidence": [
        {
          "id": "carvalho-batista-2023-shelter",
          "role": "interpretive-context",
          "use": "Shelter and context affect shrimp responses but the study does not validate a fish-safe list.",
          "path": "/research/records/carvalho-batista-2023-shelter",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        },
        {
          "id": "rickward-2024-behaviour",
          "role": "interpretive-context",
          "use": "Behavior varies with context and cannot substitute for multigeneration recruitment outcomes.",
          "path": "/research/records/rickward-2024-behaviour",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        },
        {
          "id": "wilson-2026-quantitative-behaviour",
          "role": "interpretive-context",
          "use": "Quantified behavior does not establish juvenile survival with named fish.",
          "path": "/research/records/wilson-2026-quantitative-behaviour",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        }
      ],
      "authorities": [],
      "gaps": [
        {
          "id": "tankmate-recruitment",
          "boundary": "Predator-cue behaviour and shelter studies did not place fish with shrimp and follow colony outcomes.",
          "path": "/research/gaps/tankmate-recruitment"
        }
      ],
      "comparisons": []
    },
    {
      "id": "berried-guarantee",
      "claim": "A berried female guarantees babies",
      "supported": "an ovigerous female is carrying attached eggs.",
      "notEstablished": "the observation alone proves fertilization, viability, a hatch date or retained juveniles.",
      "nextAction": "record the first observation, egg changes, hatch window, visible young and later retained cohort as separate events.",
      "claimPath": "/care/claims/berried-guarantee",
      "guidePath": "/care/breeding",
      "dossierPath": "/research/guides/breeding",
      "evidence": [
        {
          "id": "pantaleao-2017-development",
          "role": "direct-support",
          "use": "Separates post-hatching development from the earlier observation of attached eggs.",
          "path": "/research/records/pantaleao-2017-development",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        },
        {
          "id": "tropea-2015-successive-spawns",
          "role": "direct-support",
          "use": "Measures successive reproductive events rather than treating egg attachment as guaranteed retained offspring.",
          "path": "/research/records/tropea-2015-successive-spawns",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        },
        {
          "id": "li-2025-hif1a-embryo-cleavage",
          "role": "limitation-context",
          "use": "Embryo-stage observations and molecular results do not supply a universal viability or hatch forecast.",
          "path": "/research/records/li-2025-hif1a-embryo-cleavage",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        }
      ],
      "authorities": [],
      "gaps": [],
      "comparisons": []
    },
    {
      "id": "drip-clock",
      "claim": "Every shrimp should be drip acclimated for the same amount of time",
      "supported": "source and receiving water can differ, and the opened package can continue changing during a transfer.",
      "notEstablished": "one drip rate or clock time is best for every package, water comparison and animal state.",
      "nextAction": "measure temperature and relevant dissolved-property differences, inspect the package and use a predefined transfer plan.",
      "claimPath": "/care/claims/drip-clock",
      "guidePath": "/care/acclimation",
      "dossierPath": "/research/guides/acclimation",
      "evidence": [
        {
          "id": "holroyd-2026-transport-review",
          "role": "interpretive-context",
          "use": "Reviews transport stressors while preserving the lack of a validated universal receiving protocol.",
          "path": "/research/records/holroyd-2026-transport-review",
          "sourceIntegrityState": "crossref-query-unmatched",
          "sourceIntegrityLabel": "Canonical DOI outside query result",
          "sourceIntegrityBoundary": "The record has a canonical DOI but did not appear in the broad Neocaridina query. It requires DOI-specific or alternative-registry status review before any no-update statement."
        },
        {
          "id": "santoso-2022-closed-transport",
          "role": "limitation-context",
          "use": "A defined closed-transport experiment does not compare drip rates or receiving durations.",
          "path": "/research/records/santoso-2022-closed-transport",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        },
        {
          "id": "ismandar-2020-dry-transport",
          "role": "limitation-context",
          "use": "An atypical dry chilled protocol cannot establish routine wet-bag acclimation timing.",
          "path": "/research/records/ismandar-2020-dry-transport",
          "sourceIntegrityState": "crossref-query-unmatched",
          "sourceIntegrityLabel": "Canonical DOI outside query result",
          "sourceIntegrityBoundary": "The record has a canonical DOI but did not appear in the broad Neocaridina query. It requires DOI-specific or alternative-registry status review before any no-update statement."
        }
      ],
      "authorities": [
        {
          "id": "cole-1999-ornamental-fish-shipping",
          "use": "Provides adjacent ornamental-industry receiving context without becoming Neocaridina evidence.",
          "path": "/research/authorities/cole-1999-ornamental-fish-shipping"
        },
        {
          "id": "kordon-breathing-bags",
          "use": "Governs the named packaging instructions only.",
          "path": "/research/authorities/kordon-breathing-bags"
        }
      ],
      "gaps": [
        {
          "id": "transfer-rate",
          "boundary": "No direct Neocaridina comparison was located for drops per second, transfer duration or TDS-gap formulas.",
          "path": "/research/gaps/transfer-rate"
        },
        {
          "id": "shipping-package-limits",
          "boundary": "One wet closed-transport duration experiment and one atypical dry chilled experiment measured direct Neocaridina outcomes, but neither compared or validated the Molt & Moss breather-bag package, density, weather gate and receiving protocol.",
          "path": "/research/gaps/shipping-package-limits"
        }
      ],
      "comparisons": []
    },
    {
      "id": "stocking-formula",
      "claim": "A tank can hold a fixed number of shrimp per gallon",
      "supported": "density can affect outcomes under defined systems and feeding histories.",
      "notEstablished": "tank volume alone reveals oxygen, grazing surface, waste-processing capacity, maintenance limit or sustainable reproduction.",
      "nextAction": "begin with a supportable group, measure system outcomes and set an operation-specific intervention point.",
      "claimPath": "/care/claims/stocking-formula",
      "guidePath": "/care/stocking",
      "dossierPath": "/research/guides/stocking",
      "evidence": [
        {
          "id": "vazquez-2017-density",
          "role": "direct-support",
          "use": "Directly compares defined juvenile densities under one culture design.",
          "path": "/research/records/vazquez-2017-density",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        },
        {
          "id": "viau-2020-life-cycle",
          "role": "limitation-context",
          "use": "One biofilm-based multigeneration system does not establish a universal per-gallon capacity.",
          "path": "/research/records/viau-2020-life-cycle",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        }
      ],
      "authorities": [],
      "gaps": [
        {
          "id": "carrying-capacity",
          "boundary": "A 90-day juvenile density study, field density and an unreplicated three-generation observation cannot be multiplied into a planted-tank maximum.",
          "path": "/research/gaps/carrying-capacity"
        }
      ],
      "comparisons": []
    },
    {
      "id": "colour-identity",
      "claim": "Colour proves the species or purity of a line",
      "supported": "colour and pattern are observable phenotypes that can be recorded and graded under a method.",
      "notEstablished": "one photograph or trade label proves species, genotype, pedigree, relatedness, hybrid status or genetic purity.",
      "nextAction": "preserve scientific-name claim, identification basis, seller label, source, grade and provenance separately.",
      "claimPath": "/care/claims/colour-identity",
      "guidePath": "/care/species",
      "dossierPath": "/research/guides/species",
      "evidence": [
        {
          "id": "schoolmann-2018-population",
          "role": "interpretive-context",
          "use": "Population identity requires evidence beyond visible aquarium color.",
          "path": "/research/records/schoolmann-2018-population",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        },
        {
          "id": "cai-ng-1999-serrata-revision",
          "role": "direct-support",
          "use": "Shows that specimen-based taxonomic revision can overturn a historical genus label.",
          "path": "/research/records/cai-ng-1999-serrata-revision",
          "sourceIntegrityState": "crossref-query-unmatched",
          "sourceIntegrityLabel": "Canonical DOI outside query result",
          "sourceIntegrityBoundary": "The record has a canonical DOI but did not appear in the broad Neocaridina query. It requires DOI-specific or alternative-registry status review before any no-update statement."
        },
        {
          "id": "kusmintarsih-2023-market-co1",
          "role": "limitation-context",
          "use": "A short mitochondrial market-stock record does not establish pedigree, purity or all species alternatives.",
          "path": "/research/records/kusmintarsih-2023-market-co1",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        }
      ],
      "authorities": [
        {
          "id": "decanet-neocaridina-davidi",
          "use": "Supplies the maintained accepted-name database role without identifying a photographed animal.",
          "path": "/research/authorities/decanet-neocaridina-davidi"
        },
        {
          "id": "uf-ifas-cherry-shrimp-profile",
          "use": "Provides a bounded secondary species overview rather than line ancestry.",
          "path": "/research/authorities/uf-ifas-cherry-shrimp-profile"
        }
      ],
      "gaps": [
        {
          "id": "named-morph-crosses",
          "boundary": "Molecular pigment studies do not provide inheritance ratios for trade lines, and seller charts are not pedigrees.",
          "path": "/research/gaps/named-morph-crosses"
        }
      ],
      "comparisons": [
        {
          "id": "colour-phenotype-and-identity",
          "title": "Visible colour can change without proving inherited identity",
          "path": "/research/disagreements/colour-phenotype-and-identity"
        },
        {
          "id": "taxonomy-name-history",
          "title": "A published species name is evidence context, not permanent identity",
          "path": "/research/disagreements/taxonomy-name-history"
        }
      ]
    },
    {
      "id": "copper-absolute",
      "claim": "Any copper kills shrimp or copper is safe",
      "supported": "copper is biologically relevant and can be toxic; effects depend on concentration, chemical form, water chemistry, exposure and endpoint.",
      "notEstablished": "an ingredient name alone proves a product is lethal or safe, and one acute value is not a home target.",
      "nextAction": "identify the product, dose, copper fraction, water context and measured animal outcome before acting.",
      "claimPath": "/care/claims/copper-absolute",
      "guidePath": "/care/chemical-safety",
      "dossierPath": "/research/guides/chemical-safety",
      "evidence": [
        {
          "id": "li-2015-copper-chlorpyrifos",
          "role": "direct-support",
          "use": "Measures defined copper and mixture exposures with stated endpoints rather than an ingredient-only verdict.",
          "path": "/research/records/li-2015-copper-chlorpyrifos",
          "sourceIntegrityState": "crossref-query-unmatched",
          "sourceIntegrityLabel": "Canonical DOI outside query result",
          "sourceIntegrityBoundary": "The record has a canonical DOI but did not appear in the broad Neocaridina query. It requires DOI-specific or alternative-registry status review before any no-update statement."
        },
        {
          "id": "kim-2018-copper-transcripts",
          "role": "direct-support",
          "use": "Measures copper-associated transcript responses without establishing a home safe or lethal product rule.",
          "path": "/research/records/kim-2018-copper-transcripts",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        },
        {
          "id": "huang-chen-2004-juvenile-chlordane-lindane",
          "role": "limitation-context",
          "use": "Another chemical series illustrates why concentration, duration, life stage and endpoint must remain explicit.",
          "path": "/research/records/huang-chen-2004-juvenile-chlordane-lindane",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        }
      ],
      "authorities": [
        {
          "id": "epa-aquatic-life-copper",
          "use": "Provides copper essentiality, toxicity and ambient-criteria context without a Neocaridina product limit.",
          "path": "/research/authorities/epa-aquatic-life-copper"
        },
        {
          "id": "epa-copper-biotic-ligand-model",
          "use": "Shows why water chemistry changes modeled copper bioavailability.",
          "path": "/research/authorities/epa-copper-biotic-ligand-model"
        }
      ],
      "gaps": [
        {
          "id": "treatment-efficacy",
          "boundary": "One three-aquarium conference abstract reported complete Deugesia removal and three-day survival of all 12 shrimp after mebendazole or albendazole additions, but it omitted aquarium volume, usable concentration, replicated vessels, explicit control results, sublethal endpoints and recurrence follow-up. No controlled efficacy-and-safety trial was located for common hobby salt, fenbendazole, praziquantel or peroxide doses against named Neocaridina health agents.",
          "path": "/research/gaps/treatment-efficacy"
        }
      ],
      "comparisons": [
        {
          "id": "chemical-response-and-safe-threshold",
          "title": "A biological response is not automatically a care threshold",
          "path": "/research/disagreements/chemical-response-and-safe-threshold"
        }
      ]
    },
    {
      "id": "quarantine-clock",
      "claim": "A fixed quarantine period proves shrimp are clean",
      "supported": "separating each new source contact network reduces opportunities to expose established animals and creates an observation window.",
      "notEstablished": "one duration detects or clears every organism, proves pathogen-free status or validates routine prophylactic treatment.",
      "nextAction": "use a commissioned separate system, dedicated wet equipment, recorded observations and a release decision.",
      "claimPath": "/care/claims/quarantine-clock",
      "guidePath": "/care/quarantine",
      "dossierPath": "/research/guides/quarantine",
      "evidence": [
        {
          "id": "maciaszek-2023-epibionts",
          "role": "direct-support",
          "use": "Documents identified epibionts and observation limits without validating one clearance interval.",
          "path": "/research/records/maciaszek-2023-epibionts",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        },
        {
          "id": "niwa-2014-holtodrilus-microhabitat",
          "role": "direct-support",
          "use": "Documents host-associated worm observations while leaving transmission probability and quarantine duration unresolved.",
          "path": "/research/records/niwa-2014-holtodrilus-microhabitat",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        },
        {
          "id": "schneider-2022-microsporidians",
          "role": "direct-support",
          "use": "Documents molecularly examined microsporidian material without creating a universal visual screen or treatment.",
          "path": "/research/records/schneider-2022-microsporidians",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        }
      ],
      "authorities": [
        {
          "id": "fao-aquatic-quarantine",
          "use": "Supports risk-based aquatic quarantine principles without a universal Neocaridina duration.",
          "path": "/research/authorities/fao-aquatic-quarantine"
        },
        {
          "id": "woah-aquaculture-biosecurity",
          "use": "Supports movement and contact-network biosecurity principles.",
          "path": "/research/authorities/woah-aquaculture-biosecurity"
        }
      ],
      "gaps": [
        {
          "id": "treatment-efficacy",
          "boundary": "One three-aquarium conference abstract reported complete Deugesia removal and three-day survival of all 12 shrimp after mebendazole or albendazole additions, but it omitted aquarium volume, usable concentration, replicated vessels, explicit control results, sublethal endpoints and recurrence follow-up. No controlled efficacy-and-safety trial was located for common hobby salt, fenbendazole, praziquantel or peroxide doses against named Neocaridina health agents.",
          "path": "/research/gaps/treatment-efficacy"
        }
      ],
      "comparisons": []
    },
    {
      "id": "mixed-colours",
      "claim": "Mixed colours always turn brown or clear in two generations",
      "supported": "mixing undocumented breeding populations can reduce consistency when selection and records stop.",
      "notEstablished": "every named-line cross has one phenotype or reaches it on a universal F1 or F2 calendar.",
      "nextAction": "choose display or breeding as the goal, record parents and follow all offspring beyond one generation.",
      "claimPath": "/care/claims/mixed-colours",
      "guidePath": "/care/colours",
      "dossierPath": "/research/guides/colours",
      "evidence": [
        {
          "id": "tropea-2022-sibling-mating",
          "role": "interpretive-context",
          "use": "Shows that documented relatedness and reproduction require actual crosses and outcomes.",
          "path": "/research/records/tropea-2022-sibling-mating",
          "sourceIntegrityState": "crossref-query-unmatched",
          "sourceIntegrityLabel": "Canonical DOI outside query result",
          "sourceIntegrityBoundary": "The record has a canonical DOI but did not appear in the broad Neocaridina query. It requires DOI-specific or alternative-registry status review before any no-update statement."
        },
        {
          "id": "sganga-2019-maternal-colour",
          "role": "interpretive-context",
          "use": "Measures maternal coloration and offspring endpoints without producing named-line segregation ratios.",
          "path": "/research/records/sganga-2019-maternal-colour",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        },
        {
          "id": "lu-2022-chromatophore-development",
          "role": "interpretive-context",
          "use": "Describes chromatophore development in three strains without predicting arbitrary named-line crosses.",
          "path": "/research/records/lu-2022-chromatophore-development",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        }
      ],
      "authorities": [
        {
          "id": "decanet-neocaridina-davidi",
          "use": "Keeps accepted species identity separate from trade-line inheritance.",
          "path": "/research/authorities/decanet-neocaridina-davidi"
        }
      ],
      "gaps": [
        {
          "id": "named-morph-crosses",
          "boundary": "Molecular pigment studies do not provide inheritance ratios for trade lines, and seller charts are not pedigrees.",
          "path": "/research/gaps/named-morph-crosses"
        }
      ],
      "comparisons": [
        {
          "id": "colour-phenotype-and-identity",
          "title": "Visible colour can change without proving inherited identity",
          "path": "/research/disagreements/colour-phenotype-and-identity"
        }
      ]
    },
    {
      "id": "warmer-faster",
      "claim": "Warmer water always makes shrimp grow and breed better",
      "supported": "temperature can change development, growth, sex allocation, reproductive timing and physiology under tested conditions.",
      "notEstablished": "the fastest measured outcome is the best long-term welfare or colony temperature, or that one successful treatment defines a universal optimum.",
      "nextAction": "separate the desired endpoint, tested range and house operating choice, then monitor the whole colony rather than speed alone.",
      "claimPath": "/care/claims/warmer-faster",
      "guidePath": "/care/temperature",
      "dossierPath": "/research/guides/temperature",
      "evidence": [
        {
          "id": "tropea-2015-temperature",
          "role": "direct-support",
          "use": "Directly compares temperature-associated growth, reproduction and composition under the tested design.",
          "path": "/research/records/tropea-2015-temperature",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        },
        {
          "id": "balina-2018-high-temperature",
          "role": "direct-support",
          "use": "Shows that high-temperature reproductive and egg-quality endpoints can diverge from a simple faster-is-better claim.",
          "path": "/research/records/balina-2018-high-temperature",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        },
        {
          "id": "serezli-2017-temperature-sex-ratio",
          "role": "direct-support",
          "use": "Measures temperature-associated offspring sex allocation under its specific conditions.",
          "path": "/research/records/serezli-2017-temperature-sex-ratio",
          "sourceIntegrityState": "no-canonical-doi",
          "sourceIntegrityLabel": "No canonical DOI in registry",
          "sourceIntegrityBoundary": "The evidence record uses another stable source URL. Crossref DOI update metadata cannot audit it."
        },
        {
          "id": "tomas-2021-temperature",
          "role": "direct-support",
          "use": "Measures color, biochemical and spermatophore endpoints across tested temperatures rather than universal welfare.",
          "path": "/research/records/tomas-2021-temperature",
          "sourceIntegrityState": "crossref-query-match",
          "sourceIntegrityLabel": "Matched in dated Crossref query",
          "sourceIntegrityBoundary": "The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed."
        }
      ],
      "authorities": [],
      "gaps": [],
      "comparisons": [
        {
          "id": "temperature-outcomes",
          "title": "Temperature results depend on the outcome being measured",
          "path": "/research/disagreements/temperature-outcomes"
        }
      ]
    }
  ]
}
