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Time-associated RNA does not identify a best feeding hour
The evidence library is allowed to change in public. This page preserves the revised boundary, why it moved and which guides were affected.
What changed
The feeding, behaviour, molting and temperature evidence boundary now includes a complete diurnal hepatopancreas transcriptome study without turning molecular timing into a care schedule.
Why it changed
The study sampled four sequential times after at least 48 hours without feed, but time changed together with light state, a daily temperature swing and fasting duration. Its ten sequencing libraries were unequal across times, two intended libraries were unexplained, the qRT-PCR denominator conflicts with its figure and only Clk differed significantly among four validation targets. No feeding hour, intake, digestion, growth, molt, reproduction or health outcome was compared.
Sources reviewed for this change
- https://doi.org/10.2306/scienceasia1513-1874.2024.043
- https://www.scienceasia.org/2024.50.n2/scias50_2024043.pdf
- https://www.ncbi.nlm.nih.gov/bioproject/PRJNA832034
Affected practical guides
A public change is not automatically a retraction. A source may be added, a scope narrowed, a gap refined or an error corrected. The current guide and registry record own the current wording.
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