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Controlled experiment Direct Neocaridina evidence

Estudos do comportamento do camarão Neocaridina davidi var red quando exposto a diferentes espetros de luz

Marques, 2018. Integrated MSc dissertation in Veterinary Medicine, University of Lisbon.

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This record is a claim boundary, not a quality badge. Evidence class and species relationship describe what was studied. They do not make the result universal, complete or automatically applicable to a home aquarium.

Source taxon as published
Commercial adult shrimp labelled Neocaridina davidi var red; no morphological or molecular identification method was reported
Environment
Four 15-litre aquaria at a reported 23 C, pH 7 and 60 mg/L GH, each holding 15 adults and two Petri dishes of black stones, with only the left dish illuminated by a white, blue, red or green Marina LED
Life stages
commercial adult, unquantified births and molts observed during the trials
Reviewed
2026-08-12

What was studied

Study shape: The first four-week trial placed a new 15-shrimp group in each of four aquaria each week while one lamp colour remained assigned to each aquarium. The second 20-day trial kept four 15-shrimp groups in their aquaria while rotating the four lamps every five days. An observer counted shrimp inside the lit Petri dish, inside the unlit Petri dish and elsewhere every five minutes during five one-hour periods per day. Lamp spectra were reported as relative spectral distributions, and the lit-dish count was analysed with a generalized linear mixed model followed by Tukey comparisons.

Experimental unit: In the first trial, aquarium and light colour were completely confounded even though four successive shrimp groups entered each tank. In the second, aquarium-period was the assigned unit, giving four five-day tank-periods per colour while the same four groups were counted repeatedly. The thesis reports 5,200 count occasions per trial but does not state the model formula, response family, random effects, repeated-measures correlation, lamp order or carryover treatment.

Endpoints: number inside the lit Petri dish, number inside the unlit Petri dish, number elsewhere in the aquarium, qualitative movement, observed mortality, births and molts.

Claim boundary

What it can support: Under this two-dish apparatus, the mean number counted inside the lit dish differed by lamp colour in both trials. Reported lit-dish means were 3.70 red, 1.76 green, 0.93 white and 0.03 blue in the first trial, and 4.03 red, 1.74 green, 1.32 white and 0.01 blue in the rotating-lamp trial. The result supports a short-term colour-associated location-count signal that merits a better controlled replication.

What it cannot support: The thesis does not establish a preferred aquarium spectrum or a welfare ranking. It compared one coloured lit area with one dark area rather than offering simultaneous colour choices, kept the lit area on the left, did not measure or equalize irradiance or illuminance, reported only normalized spectral shapes and did not report observer blinding. Red shrimp can also differ in detectability under different coloured lamps. The stated 20:00 to 08:00 light period does not overlap the stated 09:00 to 19:00 observations, leaving the exposure timing internally unresolved. The first-trial methods state 240 adults while the abstract states n = 120. Phase one cannot separate lamp colour from aquarium; phase two does not report lamp sequence, washout or carryover control; and the statistical formula and random effects are absent despite thousands of correlated repeat counts. Sex, individual identity, lamp output drift and births entering counts were not resolved. No stress biomarker, growth, survival comparison, reproductive rate or chronic colony outcome was measured. Absence of observed deaths and incidental births or molts cannot show comfort, health benefit or reproductive superiority. Do not recommend red light, warn against blue light, specify a spectrum or photoperiod, infer colour vision, or use lamp attraction as a capture method from this study.

Publication status audit

No canonical DOI in registry

The evidence record uses another stable source URL. Crossref DOI update metadata cannot audit it.

Metadata snapshot reviewed 2026-08-12. Inspect the method, unmatched queue and limitations.

Correction impact

If this source boundary changes, these are the known downstream consumers. The list distinguishes scientific re-review from generated parity and historical follow-up.

Editorial review required (7)

This curated wording or synthesis must be reconsidered when the source boundary changes. A passing generator test cannot decide the new scientific meaning.

  • behaviour decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • habitat decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • behaviour practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • habitat practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • light-environment knowledge gap Canonical knowledge gap
    The current knowledge-gap boundary depends on a dated search that used this exact source and must be reconsidered if the source meaning changes.
  • light-environment answerability brief Answerability brief
    The decision, endpoint, minimum design or closure rule may change when evidence used in the linked gap search is corrected.
  • light-environment research allocation Current research allocation
    The current allocation, activation trigger, next permitted unit or stop decision may change when evidence used in the linked gap search is corrected.

Generated from governing registry (13)

This surface derives from a governing registry and should update with it, but release tests must still prove parity and routing.

Historical record needs follow-up decision (2)

Do not silently rewrite history. Decide whether the prior change record remains accurate and publish a new correction or scope note when needed.

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How this record is classified

Controlled experiment: An assigned treatment or controlled comparison. The treatment, unit, duration and endpoint still limit the claim.

Direct Neocaridina evidence: The source measured animals named within Neocaridina. Direct still does not mean universal or sufficient.

Topics: Habitat, Behaviour, Colour, Health

Critically appraise this source without a score

These questions fit this record's controlled experiment role. They are prompts, not automatic judgments. Open the original source to answer them.

  1. Identity and provenance: How were the organism, population, stock and life stage identified and sourced? A published name or seller label can hide a taxonomic, population or life-stage transfer.
  2. Independent unit: What unit was independently assigned or sampled, and how many units support each comparison? Animal counts do not create independent replication when animals share a vessel, site, family or treatment history.
  3. Comparison and context: What was the actual comparator, allocation process, environment, duration and material condition? A result has meaning only against the comparison and conditions that produced it.
  4. Endpoint and measurement: Which endpoint was measured, with what method, unit, timing, resolution and decision rule? One endpoint cannot silently become survival, welfare, diagnosis, reproduction or long-term population performance.
  5. Denominators and missingness: Are starting counts, exclusions, losses, missing observations and analysis denominators reconciled? Unreported or changing denominators can alter the apparent direction, precision and applicability of a result.
  6. Transfer boundary: What is the nearest tempting aquarium claim that this source design cannot establish? Direct evidence can still be narrow, and adjacent evidence can be useful only while the inference remains visible.
  7. Treatment assignment: Were independent units assigned to treatments, and were baseline conditions comparable? Unclear allocation or baseline imbalance can confound the treatment comparison.
  8. Replication: Were treatment vessels or other exposure units independently replicated rather than subsampled? Repeated animals or assays inside one exposure unit do not replicate the environmental treatment.
  9. Exposure verification: Was the treatment, dose, feed intake or environmental exposure measured and maintained as described? Nominal treatment labels may not equal the exposure animals actually received.
  10. Analysis: Did the statistical model match the unit, repeated measures, multiplicity and missing outcomes? A precise p-value cannot repair a mismatched unit or unaccounted comparison structure.

Read the complete appraisal framework.

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