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Morphology or taxonomy Direct Neocaridina evidence

Post-embryonic tail development through molting of the freshwater shrimp Neocaridina denticulata

Adachi et al., 2025. iScience 28(2): 111885.

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This record is a claim boundary, not a quality badge. Evidence class and species relationship describe what was studied. They do not make the result universal, complete or automatically applicable to a home aquarium.

Source taxon as published
Commercial Neocaridina denticulata (Neocaridina davidi), identified with partial CO1 sequence evidence
Environment
Commercial stock maintained in 45-litre tanks at 23 to 25 C; separated egg-carrying animals and hatchlings were examined in bottles, 300-microlitre 96-well plate units or invasive microscopy preparations
Life stages
embryo, first-instar hatchling, second instar after first ecdysis
Reviewed
2026-08-12

What was studied

Study shape: A post-hatching time series combined light and time-lapse imaging, morphometrics, SEM, TEM, toluidine-blue sections, confocal cuticle and cell staining, and a separate WGA-injected, UV-resin-immobilized live-imaging method across the first ecdysis.

Experimental unit: Individual shrimp within each method: 15 animals for tail length and width, three per stage for SEM, two per stage for TEM, three or more per fluorescence study, and two animals with 17 tracked nodes for resin-immobilized live imaging; Videos S1 and S2 show the same 96-well individual

Endpoints: external uropod and telson form, telson length and width, cuticle branching and furrows, epithelial cell shape, time-lapse tail dynamics through first ecdysis.

Claim boundary

What it can support: Under the tested developmental conditions, cuticle already separated overlapping uropod and telson primordia shortly after hatching. The first ecdysis made the uropod branches externally clear and changed the telson toward a longer, narrower form, with the visible tail-shape transition occurring within minutes in the recorded individual.

What it cannot support: This is first-instar developmental anatomy, not an adult molt interval or a home-visible molt predictor. The stock was commercially sourced, exact sex was unknown and the authors retained both N. denticulata and N. davidi because the name is disputed. Samples were small and method-specific. Fixed observations required anesthesia, dissection, fixation or staining. Captured 96-well animals molted faster, probably because of microscope heat. Live imaging used WGA injection, 395 nm curing light and heat-generating UV resin, slightly delayed ecdysis, included only two animals, did not label every cell and may have produced injury-related cells. The study did not test aquarium temperature, GH, minerals, treatment, survival, welfare or incomplete-molt prevention.

Publication status audit

Matched in dated Crossref query

The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed.

Registered version relationships: 10.1101/2024.03.13.583832. A preprint relationship is publication lineage, not independent replication.

Metadata snapshot reviewed 2026-08-12. Inspect the method, unmatched queue and limitations.

Correction impact

If this source boundary changes, these are the known downstream consumers. The list distinguishes scientific re-review from generated parity and historical follow-up.

Editorial review required (4)

This curated wording or synthesis must be reconsidered when the source boundary changes. A passing generator test cannot decide the new scientific meaning.

  • anatomy decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • molting decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • anatomy practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • molting practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.

Generated from governing registry (13)

This surface derives from a governing registry and should update with it, but release tests must still prove parity and routing.

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How this record is classified

Morphology or taxonomy: Anatomy, specimen identification or taxonomic relationships were studied with stated methods. A trade name or photograph cannot replace those methods.

Direct Neocaridina evidence: The source measured animals named within Neocaridina. Direct still does not mean universal or sufficient.

Topics: Anatomy, Molting, Growth and lifespan

Critically appraise this source without a score

These questions fit this record's morphology or taxonomy role. They are prompts, not automatic judgments. Open the original source to answer them.

  1. Identity and provenance: How were the organism, population, stock and life stage identified and sourced? A published name or seller label can hide a taxonomic, population or life-stage transfer.
  2. Independent unit: What unit was independently assigned or sampled, and how many units support each comparison? Animal counts do not create independent replication when animals share a vessel, site, family or treatment history.
  3. Comparison and context: What was the actual comparator, allocation process, environment, duration and material condition? A result has meaning only against the comparison and conditions that produced it.
  4. Endpoint and measurement: Which endpoint was measured, with what method, unit, timing, resolution and decision rule? One endpoint cannot silently become survival, welfare, diagnosis, reproduction or long-term population performance.
  5. Denominators and missingness: Are starting counts, exclusions, losses, missing observations and analysis denominators reconciled? Unreported or changing denominators can alter the apparent direction, precision and applicability of a result.
  6. Transfer boundary: What is the nearest tempting aquarium claim that this source design cannot establish? Direct evidence can still be narrow, and adjacent evidence can be useful only while the inference remains visible.
  7. Examined material: Which specimens, localities, sexes, stages, types or sequence accessions were actually examined? A diagnosis applies to examined material and methods, not automatically to a trade-line photograph.
  8. Diagnostic characters: Which characters or loci distinguish the taxon, and were alternatives explicitly compared? Color alone may be variable, convergent or unrelated to the diagnostic character.
  9. Name history: Were synonyms, historical combinations, type material and later revisions checked? An older title can describe a population that later work placed in another taxon.
  10. Consumer identification limit: Can the required character or method be observed reliably in an ordinary live-animal image? A valid taxonomic method may still be unavailable to a keeper examining one photograph.

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