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Controlled experiment Direct Neocaridina evidence

Investigating the Expression and Function of HIF-1alpha in Neocaridina davidi During Embryo Cleavage Stage

Li et al., 2025. Turkish Journal of Fisheries and Aquatic Sciences 25(2): TRJFAS25726.

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This record is a claim boundary, not a quality badge. Evidence class and species relationship describe what was studied. They do not make the result universal, complete or automatically applicable to a home aquarium.

Source taxon as published
Aquaculture-farm Neocaridina davidi; identification method not reported
Environment
Farm-sourced adults maintained at 27 C with daily compound feed; dsHIF-1alpha and dsEGFP groups occupied mesh boxes within the same shrimp tank, and dissolved oxygen and other water chemistry were not reported
Life stages
selected adult male, selected adult female with developed gonad, attached embryo, removed cleavage-stage embryo, removed blastula-stage embryo, later staged embryo
Reviewed
2026-08-12

What was studied

Study shape: The authors sequenced HIF-1alpha cDNA and profiled mRNA and protein across three gonad stages and seven named embryo stages. They then randomly assigned nominal groups of 300 females and 100 males to intramuscular injection of 600 nL dsHIF-1alpha or dsEGFP. Egg-carrying females observed within three days supplied maternal muscle and removed embryos for qPCR, western blots, glycolytic-gene assays and photographed developmental examples.

Experimental unit: Individual injected adult for assignment, but each treatment occupied a mesh box within one shared-water tank. Embryo expression samples pooled material from three shrimp and reported three repetitions without fully mapping females, broods, embryo counts or pools to each assay. The developmental figure shows one control sequence and three affected examples without the eligible female, clutch or embryo denominator.

Endpoints: HIF-1alpha cDNA sequence and predicted domains, stage-associated HIF-1alpha mRNA and protein, maternal muscle and embryo HIF-1alpha after dsRNA injection, embryo PFK, HK and PGK transcripts, photographed cleavage progression or arrest.

Claim boundary

What it can support: Under the reported assays, HIF-1alpha mRNA and protein were highest at the cleavage stage relative to later named embryo stages. Maternal dsHIF-1alpha injection was associated with lower HIF-1alpha signal in maternal muscle and sampled embryos, lower expression of selected glycolytic transcripts in several cleavage or blastula comparisons, and photographed examples of delayed or arrested early development relative to the dsEGFP sequence.

What it cannot support: The study did not assign oxygen treatments or measure dissolved oxygen, so the HIF name and pathway cannot establish aquarium hypoxia, embryo oxygen demand, aeration need, tolerance or a safe oxygen threshold. Shared tank water prevents independent environmental replication. Taxon identification was not reported, and the methods repeatedly call the shrimp crayfish. The number of egg-carrying females, clutches, embryos, affected embryos, surviving embryos and hatches was not reported, so the developmental frequency and effect size are unknown. Removed-embryo observation conditions and the selection of photographed examples were not fully described. The study did not directly label or trace dsRNA into oocytes or embryos, include an uninjected control, use a second non-overlapping HIF-1alpha dsRNA, report a rescue experiment or measure maternal survival and reproductive output; lower embryo signal therefore does not by itself prove physical intergenerational transfer or exclude off-target and maternal-condition pathways. Sample pooling and biological-replicate mapping are incomplete. Paired t-tests were used for apparently separate treatment groups and repeated stage comparisons without a reported multiplicity correction. Methods define significance at p less than 0.01, text uses p less than 0.05, captions introduce one, two and three-star levels, Figure 6 calls embryo-stage measurements muscle, and Figure 4 is titled protein expression although one panel is mRNA. The seven stage names partly borrow from Macrobrachium literature, and the representative 428-hour control sequence cannot supply a universal hatch countdown or viability test.

Publication status audit

Matched in dated Crossref query

The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed.

Metadata snapshot reviewed 2026-08-12. Inspect the method, unmatched queue and limitations.

Correction impact

If this source boundary changes, these are the known downstream consumers. The list distinguishes scientific re-review from generated parity and historical follow-up.

Editorial review required (10)

This curated wording or synthesis must be reconsidered when the source boundary changes. A passing generator test cannot decide the new scientific meaning.

  • A berried female guarantees babies Curated claim check
    The exact claim trace uses this record as limitation context: Embryo-stage observations and molecular results do not supply a universal viability or hatch forecast.
  • breeding decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • filtration decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • sexing decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • breeding practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • filtration practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • sexing practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • dissolved-oxygen knowledge gap Canonical knowledge gap
    The current knowledge-gap boundary depends on a dated search that used this exact source and must be reconsidered if the source meaning changes.
  • dissolved-oxygen answerability brief Answerability brief
    The decision, endpoint, minimum design or closure rule may change when evidence used in the linked gap search is corrected.
  • dissolved-oxygen research allocation Current research allocation
    The current allocation, activation trigger, next permitted unit or stop decision may change when evidence used in the linked gap search is corrected.

Generated from governing registry (19)

This surface derives from a governing registry and should update with it, but release tests must still prove parity and routing.

Historical record needs follow-up decision (2)

Do not silently rewrite history. Decide whether the prior change record remains accurate and publish a new correction or scope note when needed.

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How this record is classified

Controlled experiment: An assigned treatment or controlled comparison. The treatment, unit, duration and endpoint still limit the claim.

Direct Neocaridina evidence: The source measured animals named within Neocaridina. Direct still does not mean universal or sufficient.

Topics: Anatomy, Breeding, Genetics, Water parameters

Critically appraise this source without a score

These questions fit this record's controlled experiment role. They are prompts, not automatic judgments. Open the original source to answer them.

  1. Identity and provenance: How were the organism, population, stock and life stage identified and sourced? A published name or seller label can hide a taxonomic, population or life-stage transfer.
  2. Independent unit: What unit was independently assigned or sampled, and how many units support each comparison? Animal counts do not create independent replication when animals share a vessel, site, family or treatment history.
  3. Comparison and context: What was the actual comparator, allocation process, environment, duration and material condition? A result has meaning only against the comparison and conditions that produced it.
  4. Endpoint and measurement: Which endpoint was measured, with what method, unit, timing, resolution and decision rule? One endpoint cannot silently become survival, welfare, diagnosis, reproduction or long-term population performance.
  5. Denominators and missingness: Are starting counts, exclusions, losses, missing observations and analysis denominators reconciled? Unreported or changing denominators can alter the apparent direction, precision and applicability of a result.
  6. Transfer boundary: What is the nearest tempting aquarium claim that this source design cannot establish? Direct evidence can still be narrow, and adjacent evidence can be useful only while the inference remains visible.
  7. Treatment assignment: Were independent units assigned to treatments, and were baseline conditions comparable? Unclear allocation or baseline imbalance can confound the treatment comparison.
  8. Replication: Were treatment vessels or other exposure units independently replicated rather than subsampled? Repeated animals or assays inside one exposure unit do not replicate the environmental treatment.
  9. Exposure verification: Was the treatment, dose, feed intake or environmental exposure measured and maintained as described? Nominal treatment labels may not equal the exposure animals actually received.
  10. Analysis: Did the statistical model match the unit, repeated measures, multiplicity and missing outcomes? A precise p-value cannot repair a mismatched unit or unaccounted comparison structure.

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