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Controlled experiment Direct Neocaridina evidence

Brothers are better than nothing: first report of incestuous mating and inbreeding depression in a freshwater decapod crustacean

Tropea, Marciano and López Greco, 2022. Zoology 151: 125990.

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This record is a claim boundary, not a quality badge. Evidence class and species relationship describe what was studied. They do not make the result universal, complete or automatically applicable to a home aquarium.

Source taxon as published
Neocaridina davidi
Environment
Laboratory sibling and non-sibling mating comparison
Life stages
adult breeder, egg, newly hatched juvenile, juvenile
Reviewed
2026-08-12

What was studied

Study shape: Females were paired with brothers or non-brothers and offspring were followed through separate brood and juvenile endpoints.

Experimental unit: Mating pair or offspring culture group, depending on endpoint

Endpoints: mating, egg count, hatch count, juvenile survival, growth under feeding treatments.

Claim boundary

What it can support: Mating and early egg measures can look similar while later hatch and juvenile outcomes differ after one full-sibling generation.

What it cannot support: It does not establish a founder minimum, multigeneration decline, colour loss, disease effect or calendar-based outcross schedule.

Publication status audit

Canonical DOI outside query result

The record has a canonical DOI but did not appear in the broad Neocaridina query. It requires DOI-specific or alternative-registry status review before any no-update statement.

Metadata snapshot reviewed 2026-08-12. Inspect the method, unmatched queue and limitations.

Correction impact

If this source boundary changes, these are the known downstream consumers. The list distinguishes scientific re-review from generated parity and historical follow-up.

Editorial review required (8)

This curated wording or synthesis must be reconsidered when the source boundary changes. A passing generator test cannot decide the new scientific meaning.

  • Mixed colours always turn brown or clear in two generations Curated claim check
    The exact claim trace uses this record as interpretive context: Shows that documented relatedness and reproduction require actual crosses and outcomes.
  • breeding decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • colours decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • genetics decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • breeding practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • colours practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • genetics practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • round1-taxonomy-lineage Curated first-round reviewer assignment
    The frozen reviewer scope or consequential-failure check uses this record through the assigned claim trace or comparison and must be reconsidered before invitation or response.

Generated from governing registry (19)

This surface derives from a governing registry and should update with it, but release tests must still prove parity and routing.

Inspect the corpus-wide propagation audit.

How this record is classified

Controlled experiment: An assigned treatment or controlled comparison. The treatment, unit, duration and endpoint still limit the claim.

Direct Neocaridina evidence: The source measured animals named within Neocaridina. Direct still does not mean universal or sufficient.

Topics: Genetics, Breeding, Growth and lifespan, Feeding

Critically appraise this source without a score

These questions fit this record's controlled experiment role. They are prompts, not automatic judgments. Open the original source to answer them.

  1. Identity and provenance: How were the organism, population, stock and life stage identified and sourced? A published name or seller label can hide a taxonomic, population or life-stage transfer.
  2. Independent unit: What unit was independently assigned or sampled, and how many units support each comparison? Animal counts do not create independent replication when animals share a vessel, site, family or treatment history.
  3. Comparison and context: What was the actual comparator, allocation process, environment, duration and material condition? A result has meaning only against the comparison and conditions that produced it.
  4. Endpoint and measurement: Which endpoint was measured, with what method, unit, timing, resolution and decision rule? One endpoint cannot silently become survival, welfare, diagnosis, reproduction or long-term population performance.
  5. Denominators and missingness: Are starting counts, exclusions, losses, missing observations and analysis denominators reconciled? Unreported or changing denominators can alter the apparent direction, precision and applicability of a result.
  6. Transfer boundary: What is the nearest tempting aquarium claim that this source design cannot establish? Direct evidence can still be narrow, and adjacent evidence can be useful only while the inference remains visible.
  7. Treatment assignment: Were independent units assigned to treatments, and were baseline conditions comparable? Unclear allocation or baseline imbalance can confound the treatment comparison.
  8. Replication: Were treatment vessels or other exposure units independently replicated rather than subsampled? Repeated animals or assays inside one exposure unit do not replicate the environmental treatment.
  9. Exposure verification: Was the treatment, dose, feed intake or environmental exposure measured and maintained as described? Nominal treatment labels may not equal the exposure animals actually received.
  10. Analysis: Did the statistical model match the unit, repeated measures, multiplicity and missing outcomes? A precise p-value cannot repair a mismatched unit or unaccounted comparison structure.

Read the complete appraisal framework.

Where it is used

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