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Controlled experiment Direct Neocaridina evidence

Enhancing the color and stress tolerance of cherry shrimp using astaxanthin and Bidens pilosa

Hou et al., 2024. PLOS ONE 19(12): e0315585.

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This record is a claim boundary, not a quality badge. Evidence class and species relationship describe what was studied. They do not make the result universal, complete or automatically applicable to a home aquarium.

Source taxon as published
Neocaridina davidi var. red
Environment
Independently filtered six-litre jars at 24 C for 56 days followed by a nine-hour sealed-bottle challenge
Life stages
juvenile all-male shrimp
Reviewed
2026-08-12

What was studied

Study shape: Five diets differing in astaxanthin and Bidens pilosa were compared with repeated colour measurements, gene expression and a later sealed-bottle challenge.

Experimental unit: Independently filtered jar for the diet treatment and dissolved-oxygen bottle for the later challenge

Endpoints: CIELAB colour, colour-related gene expression, stress-related gene expression, nine-hour colour stability.

Claim boundary

What it can support: The tested additives changed measured colour values and selected gene-expression outcomes under the reported diet and challenge conditions.

What it cannot support: It does not establish genetic colour improvement, permanent grade, general health benefit, transport success or a safe dissolved-oxygen threshold.

Publication status audit

Matched in dated Crossref query

The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed.

Metadata snapshot reviewed 2026-08-12. Inspect the method, unmatched queue and limitations.

Correction impact

If this source boundary changes, these are the known downstream consumers. The list distinguishes scientific re-review from generated parity and historical follow-up.

Editorial review required (6)

This curated wording or synthesis must be reconsidered when the source boundary changes. A passing generator test cannot decide the new scientific meaning.

  • colours decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • feeding decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • filtration decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • colours practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • feeding practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • filtration practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.

Generated from governing registry (16)

This surface derives from a governing registry and should update with it, but release tests must still prove parity and routing.

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How this record is classified

Controlled experiment: An assigned treatment or controlled comparison. The treatment, unit, duration and endpoint still limit the claim.

Direct Neocaridina evidence: The source measured animals named within Neocaridina. Direct still does not mean universal or sufficient.

Topics: Feeding, Colour, Filtration, Health

Critically appraise this source without a score

These questions fit this record's controlled experiment role. They are prompts, not automatic judgments. Open the original source to answer them.

  1. Identity and provenance: How were the organism, population, stock and life stage identified and sourced? A published name or seller label can hide a taxonomic, population or life-stage transfer.
  2. Independent unit: What unit was independently assigned or sampled, and how many units support each comparison? Animal counts do not create independent replication when animals share a vessel, site, family or treatment history.
  3. Comparison and context: What was the actual comparator, allocation process, environment, duration and material condition? A result has meaning only against the comparison and conditions that produced it.
  4. Endpoint and measurement: Which endpoint was measured, with what method, unit, timing, resolution and decision rule? One endpoint cannot silently become survival, welfare, diagnosis, reproduction or long-term population performance.
  5. Denominators and missingness: Are starting counts, exclusions, losses, missing observations and analysis denominators reconciled? Unreported or changing denominators can alter the apparent direction, precision and applicability of a result.
  6. Transfer boundary: What is the nearest tempting aquarium claim that this source design cannot establish? Direct evidence can still be narrow, and adjacent evidence can be useful only while the inference remains visible.
  7. Treatment assignment: Were independent units assigned to treatments, and were baseline conditions comparable? Unclear allocation or baseline imbalance can confound the treatment comparison.
  8. Replication: Were treatment vessels or other exposure units independently replicated rather than subsampled? Repeated animals or assays inside one exposure unit do not replicate the environmental treatment.
  9. Exposure verification: Was the treatment, dose, feed intake or environmental exposure measured and maintained as described? Nominal treatment labels may not equal the exposure animals actually received.
  10. Analysis: Did the statistical model match the unit, repeated measures, multiplicity and missing outcomes? A precise p-value cannot repair a mismatched unit or unaccounted comparison structure.

Read the complete appraisal framework.

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