Transcriptome profiles of the hepatopancreas of the Chinese swamp shrimp (Neocaridina denticulata) under different diurnal rhythms
2024; high priority; method reviewed
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Adds a direct hepatopancreas time-series while separating time-associated RNA from a feeding hour, circadian mechanism, temperature effect, molt prediction or health recommendation. |
Intake complete from the official 18-page article and supplement PDF after extracted-text and every-page visual review. Preserve 3,000 market-sourced animals, a printed mean weight of 1.37 +/- 0.28 g, two weeks in recirculating tap water, a reported 12-hour light and 12-hour dark cycle, a natural daily temperature range of approximately 10 to 18 C, feed withheld for at least 48 hours, and destructive sampling at 06:00, 12:00, 18:00 and 24:00. Preserve 60 hepatopancreases per time intended as three tubes of 20, but only ten RNA-seq libraries distributed 2, 3, 3 and 2 across times with two missing intended libraries unexplained. The written pooling method is unclear. The qRT-PCR methods report three groups per time and five biological replicates per group while the figure shows three points per time; only Clk was significant among Clk, Cry, Per and Tim. Preserve that time co-varied with light state, temperature and fasting duration, and that no constant-temperature, shifted-light, constant-darkness, feeding-time, repeated-cycle or time-randomized control isolated a cause. Differential-expression screening used raw p less than 0.05 without a stated false-discovery correction, and the printed 983 total for 12:00 versus 18:00 conflicts with 588 up plus 350 down, which totals 938. The title and methods use N. denticulata while parts of the results and discussion use N. denticulata sinensis, with no host diagnostic method or voucher. Do not infer a best feeding hour, nocturnal feeding rule, endogenous circadian rhythm, light or temperature cause, fasting schedule, molt prediction, improved digestion, immunity, growth or health from pathway annotations. |
Influences of the diurnal cycle on gut microbiota in the Chinese swamp shrimp (Neocaridina denticulata)
2023; high priority; full text needed
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Adds a direct four-time gut-microbiota series while separating 16 sequencing samples from 600 source animals, descriptive diversity from a feeding schedule and bacterial genera from disease. |
The official Taylor and Francis record authenticates this 13-page article, published online 29 July 2022 in the 2023 issue, but the complete article remains access-controlled. Preserve only the abstract-reported scope: gut material from 600 Chinese swamp shrimp was sampled at 06:00, 12:00, 18:00 and 24:00 for 16S sequencing, with descriptive richness and diversity highest at 24:00, predicted digestion and absorption pathways reported as enriched, and Flavobacterium, Acinetobacter and Pseudomonas discussed as opportunistic pathogens. The official CC BY Figshare supplement, https://doi.org/10.6084/m9.figshare.20402326, contains two figures and two workbooks. Figure S1 visibly contains four sample points per time and reports no significant pairwise alpha-diversity comparison: Chao1, Shannon, Simpson and observed-species p values all range from 0.13 to 0.83. Figure S2 shows 16 rarefaction curves, four per time. Sixteen visible sequencing samples are not 600 independent microbial replicates, and a descriptively higher midnight value is not a supported time optimum. The open material does not establish how many guts formed each library, independent tank allocation, feeding schedule, light and temperature history, dissection and extraction controls, absolute bacterial load, beta-diversity model, multiplicity, pathway-prediction method or raw-sequence accession. The two official supplemental workbooks were checksum-verified but not audited because the bundled spreadsheet runtime did not initialize. Full text is required to verify source and taxon identity, tank structure, acclimation, animal-to-library construction, primers and region, sequencing, bioinformatics, controls, statistical unit and functional inference. Do not infer an endogenous circadian rhythm, best feeding hour, nocturnal care rule, measured digestion or absorption, infection, virulence, host disease, probiotic need, medication need or absolute bacterial abundance from the abstract or supplement. This DOI was one of the frozen census unmatched title records and is now a documented abstract-and-supplement decision, not evidence or a guide source. |
Neocaridina davidi (Bouvier, 1904). A bibliometric analysis
2024; high priority; method reviewed
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Provides an independent Scopus-era map of Neocaridina publishing while showing why database counts require reproducible queries and record-level reconciliation before they can support completeness claims. |
Method review completed from the official open 25-page journal viewer and the publicly indexed author-uploaded article text, with visual checks of the methods, count, country, citation-table and network pages. Preserve a Scopus corpus described as 117 publications from 1952 through 2021, 328 authors, VOSviewer 1.6.16 analysis, searches of titles and abstracts using current and historical names, and a citation snapshot dated 25 March 2024. Do not call 117 a completeness estimate. The article provides no exact Scopus field query or Boolean syntax, search execution date, language and document-type filters, deduplication procedure, reviewer count, screening flow, excluded-record list, raw CSV, thesaurus file or VOSviewer project. The methods print N. denticulata twice and do not print the full Neocaridina denticulata term shown in the abstract. Published arithmetic is internally inconsistent: document types total 107 rather than 117, access categories total 108, listed topic shares total 93 percent, and China is reported as both 43 and 37 publications in different analyses. Country percentages use an unstated denominator larger than 117, likely because coauthored papers contribute multiple country associations, but that counting rule is not explained. The simplified citation table includes at least two works not directly about Neocaridina, including a 2008 Antonie van Leeuwenhoek paper and the 2018 PeerJ whiteleg-shrimp microbiome paper, despite the stated direct-relevance inclusion rule. The PDF lists six authors while the publisher landing-page metadata and suggested citation list five, omitting Luis Manuel Guevara Chumacero. Use this source only as a directional historical and network comparison. It cannot validate the Crossref census, prove that either database is complete, establish that every record concerns N. davidi, rank evidence quality, measure hobby knowledge or support husbandry claims. This DOI was one of the frozen census unmatched titles and is now a documented method-reviewed bibliometric decision, not a biological evidence record or guide source. |
Cherry Shrimp Neocaridina davidi (Bouvier 1904) (Crustacea: Decopoda: Atyidae)
2020; high priority; method reviewed
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Audits a prominent peer-reviewed extension profile that hobby sites cite for care advice, preserving its useful species orientation while showing that it contains no aquarium parameter table. |
Method review completed from the current official three-page UF/IFAS Extension PDF, originally published March 2020 and revised June 2024, after extracted-text review and visual inspection of all pages. Preserve its intended role as a Featured Creatures profile for informed lay and academic audiences; examples of common colour labels; gross external anatomy; direct development; and a secondary overview of introduced records in Poland, Germany, Japan, Israel and Hawaii. The published title prints Decopoda rather than Decapoda. The synonym list contains N. denticulata sinensis and N. heteropoda but is not a complete current taxonomic authority. The trade-name list maps blue dream, blue velvet and blue jelly together and calls rili a striped morph without crosses, pedigrees, genetic tests, seller sampling or stable line definitions. Use those as examples of public labels, not line equivalence or ancestry. The profile contains no recommended aquarium temperature, pH, GH, KH, conductivity, TDS, ammonia, nitrite, nitrate, stocking density, tank size, water-change rate, quarantine period or feeding amount. It reports 3 to 4 cm maximum adult length, 16 to 19 days incubation, 43 to 60 eggs, 1 to 2 mm young and sexual maturity around 30 days, but only the clutch sentence cites Pantaleao et al.; the page does not expose denominators or study conditions for the other values. Its miniature-adult wording hides continued post-hatching morphological development documented in the cited primary source. Claims that shrimp eat molts to reabsorb nutrients and help keep water clean are not tied to a measured nutrient-recovery or aquarium-water endpoint. Its alder and goat-willow preference sentence relies on the Schoolmann and Arndt abstract-level paper whose complete methods remain unavailable in this review. The eight selected references are a secondary trail, not evidence that every profile sentence was independently verified. Use the extension source as professional species and no-release orientation only. Do not infer a parameter target, maturity calendar, incubation countdown, adult-size promise, complete food, cleaning capacity, stable morph taxonomy, genetic purity or safe release. This DOI was one of the frozen census unmatched titles and is now a documented method-reviewed secondary-source decision and bounded supporting authority, not a biological experiment. |
Neocaridina fonticulata, a new land-locked freshwater shrimp from Hengchun Peninsula, Taiwan (Decapoda, Caridea, Atyidae)
2019; high priority; method reviewed
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Adds a complete genus-level species description that shows why another Neocaridina species is not an aquarium colour morph and why identification may require adult sex-specific anatomy plus molecular comparison. |
Intake complete from the official CC BY 13-page ZooKeys PDF after extracted-text review and visual inspection of every page. Preserve the holotype, multiple paratype lots, additional Sheding material and comparative N. ikiensis material; rostrum, pereiopod and male first-and-second-pleopod diagnosis; egg measurements from five eggs on each of four ovigerous females; and four Sheding shrimp sequenced for a 658-base-pair mitochondrial COI segment that yielded one reported haplotype, GenBank LC427866. The smallest reported K2P distances were 5.42 percent to sampled N. ketagalan and 5.43 percent to sampled N. saccam. Preserve the source boundary: N. fonticulata was then known only from one locality, and the prediction that it could occur in eastern Taiwan was not a sampled record. The four-shrimp, one-haplotype mitochondrial result has no nuclear locus, cross, genomic ancestry test or blind identification validation. Several diagnostic structures require an adult male and specialist examination. The collection-site values of about 25 C, pH 7.06 to 7.16 and dissolved oxygen 7.33 to 7.70 mg/L lack temporal replication and assigned comparisons; they are habitat context, not aquarium targets, tolerances or optima. Aquarium-held live-colour photographs were not a care trial. Do not identify N. fonticulata or any commercial morph from colour alone, convert COI distance into a universal barcode cutoff, authenticate a seller line, claim reproductive isolation, map the complete range or infer husbandry requirements. This DOI was one of the frozen census unmatched title records and is now a documented method-reviewed taxonomy decision, evidence record and species-guide source. |
Neocaridina iriomotensis, a new species of land-locked freshwater shrimp (Crustacea: Decapoda: Atyidae) from Iriomote Island, southern Ryukyus, Japan
2006; high priority; method reviewed
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Adds a large morphology-based species description whose seasonal walking-leg difference and unresolved ecomorphs teach why a single visible character, season or photograph cannot establish Neocaridina identity. |
Intake complete from the Natural History Museum of Los Angeles County hosted seven-page article scan after extracted-text review and visual inspection of every page. Preserve one male holotype, 41 paratypes and 49 non-type specimens from seven collection dates spanning November 1999 through June 2000 at the upper reaches of Nishifunatsuki in the Nakama River. Only specimens above 4 mm carapace length were used for description. Character ratios report varying sample sizes from 73 to 83. Preserve the combination of rostrum, pterygostomian-spine, pereiopod and male pleopod characters used against published N. anhuiensis and topotypic N. ishigakiensis material. The third pereiopod showed sex-associated form in summer specimens but no sexual difference in winter specimens. Egg size was 1.05 to 1.35 by 0.73 to 0.88 mm and the reported clutch range was 21 to 82, but female, clutch and egg denominators were not provided. All listed material came from one named upper-reach locality. The claim that the shrimp was commonly found has no sampling-effort denominator. Proposed headwater ecomorph populations included intermediate rostrum lengths and remained tentative pending molecular and ecological study; this paper conducted no molecular analysis. The habitat description gives channel dimensions, slow flow, vegetation and tree roots but no temperature, pH, hardness, conductivity, dissolved oxygen or nitrogen measurements. Do not identify an aquarium shrimp from colour, one photograph or one season-sensitive appendage; authenticate a seller line; claim genetic separation, reproductive isolation, abundance or complete range; turn the clutch range into a breeding forecast; or infer water targets and care requirements. This DOI was one of the frozen census unmatched title records and is now a documented method-reviewed morphology-taxonomy decision, evidence record and species-guide source. |
Chromatosomes in Three Phenotypes of Neocaridina denticulata Kemp, 1918: Morphological and Chromatic Differences Measured Non-Invasively
2011; high priority; full text needed
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Could add a direct non-invasive colour-observation method while separating an anatomical imaging site from commercial grade, colour stability and husbandry claims. |
The official Oxford Academic record authenticates this eight-page 2011 Journal of Crustacean Biology article and exposes its abstract, but labels the article as access-controlled and the direct PDF request returned HTTP 403. Preserve only the abstract-reported scope: three phenotypes of Neocaridina denticulata under 2 cm differed in epidermal chromatosome quantity and dispersal, pigmentation was most pronounced in anterior body parts, and the uropod exopod was proposed as the most suitable area for non-invasive colour monitoring. Full text is required to verify phenotype names, animal source and identity, animal and holding-unit counts, sex, age, size distribution, molt and reproductive state, acclimation, background, light, camera and microscope settings, body regions, repeated observations, chromatosome counting and dispersal scales, calibration, blinding, statistical model, raw denominators, effect sizes and uncertainty. Do not infer a commercial grade, inheritance or genotype, health state, diet effect, preferred background, colour permanence, seller standard or phone-photo diagnostic. A suggested imaging site is not proof that one photograph measures a stable whole-animal phenotype. This DOI was one of the frozen census unmatched title records and is now a documented abstract-only decision. |
Microhabitat distribution and behaviour of Branchiobdellidan Holtodrilus truncatus found on the freshwater shrimp Neocaridina spp. from the Sugo River, Japan
2014; high priority; method reviewed
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Adds direct wild-host body-location, cocoon, detached-persistence and host-exchange observations while separating them from diagnosis, harm, treatment and quarantine-duration claims. |
Intake complete from the open six-page German National Library PDF after extracted-text and every-page visual review. Preserve 271 total wild Neocaridina spp. collected across three Sugo River stations from 2003 to 2011; mapped locations on 141 hosts in 2011; 55.3 percent between the first pleopod and fifth pereiopod, 17.0 percent carapace, 15.6 percent eye base, 8.5 percent antennule and 3.6 percent around the egg mass; 23 measured cocoons spanning 0.58 to 0.76 mm; a maximum observed 14 developing worms per cocoon; ten host-removed worms in 100 mL vials surviving 7 to 46 days and three in dishes surviving 12 to 21 days without added food; and all five supplied worms attaching to Neocaridina within three hours in the small host-exchange demonstration. Preserve that hosts were identified only as Neocaridina spp. amid discussed native and imported lineages, the total and mapped denominators are not fully reconciled, station denominators are absent, location-count construction is unclear, two of 15 survival worms are missing from the reported ranges, containers differed, no fed or host-present control and death criterion were stated, and five-worm exchange order, reuse, replication, vessel context and complete 28-host outcomes were omitted. The assertion that infested host eggs hatched healthy young has no denominator or follow-up. Do not turn a maximum 46-day detached-worm observation into a quarantine clock, infer N. davidi prevalence or transmission probability, recommend predator hosts, deliberately deteriorate hosts to collect worms, claim harmlessness or prescribe treatment. This DOI was one of the frozen census unmatched title records and is now a documented decision. |
First report of the branchiobdellidan Holtodrilus truncatus (Annelida: Clitellata) found on the freshwater atyid shrimp Neocaridina sp. from Korea
2016; high priority; method reviewed
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Adds a vouchered Korean occurrence and the first COI barcode reference for the genus while separating taxonomic detection from prevalence, host harm, trade origin and treatment. |
Intake complete from the official four-page Korea Citation Index PDF after extracted-text and every-page visual review. Preserve wild Neocaridina sp. material from one Andeok Valley stream dated 23 June 2010, 23 August 2015 and 18 May 2016; immediate 95 percent ethanol preservation; stereomicroscope removal; transparent terete worms under 2 mm; no trunk appendages; a posterior sucker about the head-region diameter; paired jaws with a 7/7 dental formula; three listed NIBR voucher identifiers; and one whole-worm 674-base-pair mitochondrial COI sequence deposited as GenBank KX683299. Preserve that host and worm counts, per-date allocation, sampling effort and absence checks were not reported; the host remained Neocaridina sp. without a stated voucher or molecular identification; only three NIBR identifiers were printed despite additional Inha University deposition; individual images, morphology, sequence and vouchers were not mapped; and the single sequence was not tested by phylogeny or species delimitation. Immediate preservation prevented live or host-response observation. Do not infer prevalence, continuous persistence, geographic range, N. davidi equivalence, native or introduced origin, aquarium-trade transmission, harmlessness, a phone-image diagnosis, quarantine duration or treatment. This DOI was one of the frozen census unmatched title records and is now a documented decision. |
Changes in activity rates in the cherry shrimp Neocaridina davidi Bouvier, 1904 (Decapoda: Caridea: Atyidae) in response to concentrations of neuropeptides and glucose
2019; high priority; full text needed
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Adds a direct N. davidi movement-response experiment while separating short chemical-exposure activity counts from hunger, stress, welfare and home feeding advice. |
The official Oxford Academic record authenticates this six-page 2019 Journal of Crustacean Biology article and exposes its abstract, but both available browser sessions report no current article access and the direct PDF endpoint returned only an access-challenge HTML page. Preserve only the abstract-reported 20-minute observations in 4-litre tanks after chemicals were applied directly to the water: MSG increased movement and became more stimulatory over time, glucose increased movement with a declining response over time, dopamine reduced movement, insulin reduced activity, insulin plus glucose showed no effect, dopamine plus glucose increased activity and dopamine plus MSG reduced activity. The abstract also says the MSG response was statistically similar to food-pellet filtrate. A related conference abstract describes groups of ten shrimp and supplies concentrations, but it is not the final article and conflicts with the article abstract wording about observations of shrimp individuals. Full text is required to verify animal source, morph and life stage, acclimation, fasting, tank number and volume, animals per tank, whether shrimp or tanks were reused, independent replication, allocation, water chemistry, treatment preparation and achieved exposure, matched vehicle controls, movement definition and coding, observer blinding, time bins, statistical model, multiplicity, raw denominators, effect sizes and uncertainty. Do not dose MSG, glucose, dopamine, insulin or haloperidol in an aquarium; call activity a direct measure of hunger, food seeking, stress or welfare; infer a feeding amount; or treat a brief pharmacological exposure as ordinary husbandry. This DOI was one of the frozen census unmatched title records and is now a documented abstract-only decision. |
Reproductive Biology of Neocaridina denticulata denticulata and Latreutes planirostris
2006; high priority; method reviewed
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Adds direct genus-level brood, egg-volume and seasonal gonad measurements while separating selected wild females from aquarium fecundity and repeated-spawning promises. |
Intake complete from the official five-page KoreaScience image PDF after bilingual OCR and every-page visual review. Preserve one wild Korean N. denticulata denticulata population collected by scoop net during 2001, fixation in 5 percent neutral formalin, selection of ovigerous females, 43 brood-count females measuring 5.69 to 7.85 mm carapace length, 47 to 117 eggs with mean 82 +/- 18, five sampled eggs per female for volume, mean egg volume 0.70 mm3, mean reproductive output 41.84 percent, monthly groups of 48 to 60 females for gonadosomatic index, and separate ovarian regressions for 35 non-eyed-stage and 48 eyed-stage females. Preserve that ovarian dry weight was higher in eyed-stage females at a common carapace-length slope, which supports rematuration during embryo development but not an observed second spawning. The paper does not report a taxonomic key or voucher, exact collection dates or effort, water temperature or chemistry, total female source denominator, annual replication, fertilization, egg retention, hatch, juvenile survival or individual repeated broods. Do not transfer a selected wild-population clutch distribution, May gonadosomatic peak or potential consecutive-spawning inference to captive N. davidi, a temperature target, a seller promise or a home-colony forecast. This DOI was one of the frozen census unmatched title records and is now a documented decision. |
Occurrence of Scutariella worms on Neocaridina davidi shrimp (Bouvier, 1904) in Hungary
2023; high priority; method reviewed
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Adds a direct captive-shrimp identification case while separating visible worm morphology from prevalence, harm, transmission and an unsupported fenbendazole recipe. |
Intake complete from the open eight-page University of Veterinary Medicine Budapest repository PDF after extracted-text and every-page visual review. Preserve 15 selected diagnostic submissions from different breeders, seven females and eight males, five shrimp in each of three approximately 18-litre holding aquaria, 2 to 8 visible worms on each shrimp head, antennae or rostral region, reported worm length of 0.8 to 2.0 mm, and eggs visible in the branchial chamber. Morphological identification used live stereomicroscopy and light microscopy with paired anterior projections, two eyespots, internal reproductive structures and a posterior horseshoe- or heart-shaped sucker. Preserve that host identity was not independently tested, source counts and clustering were omitted, the number of worms examined was not stated, and no molecular confirmation or voucher was reported. This was a selected case series without untreated controls or respiration, gill-lesion, molt, mortality, transmission, spontaneous-clearance or treatment endpoints. Harm statements came from cited literature. The one-sentence fenbendazole discussion supplied no product, dose, exposure, efficacy, recurrence or host-safety result. Do not infer prevalence, prove source transmission, identify a distant white shape, prescribe fenbendazole or use treatment response as diagnosis. This DOI was one of the frozen census unmatched title records and is now a documented decision. |
Effects of Thiacloprid on Freshwater Systems Dominated by Neocaridina Denticulate and Physa Fontinalis
2024; high priority; full text needed
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Adds a direct 28-day neonicotinoid-system lead while keeping a blocked preprint download, an unresolved taxon spelling and abstract-level NOECs out of aquarium limits. |
Crossref authenticates this as a 2024 SSRN preprint by Jilin Wang and Shao-nan Li, and the SSRN abstract reports one indoor-system application series at nominal 0.3, 0.9, 2.8, 8.3 and 25.0 micrograms/L thiacloprid with Neocaridina denticulate and Physa fontinalis. Preserve only the abstract-reported time-weighted-average wet-weight NOEC and LOEC of 0.72 and 2.7 micrograms/L with effects through 21 days, and antibody-corrected free-living chitobiase NOEC and LOEC of 1.79 and 5.49 micrograms/L with effects through 28 days. The abstract also reports stimulation of water fleas, chlorophyll-a and phycocyanin, suppression of selected bacterial functional groups and increased phosphate and ammonium. The 31-page SSRN manuscript download returned HTTP 403, and no separate peer-reviewed publication or lawful repository copy was located. Full text and supplements are required to verify whether the printed N. denticulate name means N. denticulata, animal source and identification, life stage, vessel and animal counts, independent replication, allocation, controls, nominal-versus-measured exposure series, dissipation and time-weighted-average calculation, water chemistry, feeding, endpoint definitions, antibody validation, statistical tests, multiplicity, raw denominators and uncertainty. Do not use a preprint abstract as a thiacloprid care threshold, infer N. davidi equivalence, interpret stimulation as benefit, or transfer results from the related tebufenpyrad and diflubenzuron systems. Crossref classifies this DOI as posted content rather than a journal article, so it does not change the frozen 198-title journal-article census. |
Effects of tebufenpyrad on freshwater systems dominated by Neocaridina palmata, Physa fontinalis, and Ceratophyllum demersum
2022; high priority; full text needed
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Adds a direct N. palmata acaricide-system study while separating abstract-level biomass and chitobiase endpoints from mortality, home exposure and N. davidi safety. |
Crossref, PubMed PMID 35643160 and the official Chemosphere record authenticate this CC BY article. Its abstract reports one application in indoor systems containing Neocaridina palmata, Physa fontinalis, Ceratophyllum demersum, Simocephalus vetulus and Dolerocypris sinensis. Preserve only the abstract-reported time-weighted-average wet-weight NOEC and LOEC of 0.67 and 2.33 micrograms/L with a dose-related effect lasting 21 days, and antibody-corrected free chitobiase NOEC and LOEC of 1.41 and at most 5.64 micrograms/L. The abstract reports plant suppression, stimulation of the cladoceran and ostracod, bacterial-community changes and mixed water-chemistry responses. The publisher PDF and text-mining endpoints rejected automated retrieval, while OpenAlex reports no repository full text and Europe PMC reports no PDF or supplement. Full text and supplements are required to verify shrimp identity and stock, acclimation, animal and vessel counts, replication, allocation, exposure concentrations, analytical recovery and dissipation, time-weighted-average calculation, controls, feeding, endpoint definitions and denominators, antibody correction, statistics, multiplicity and uncertainty. Wet weight and free-living chitobiase are population-production proxies, not direct mortality, individual growth or reproduction measurements. Do not convert either NOEC into an aquarium limit, assume N. palmata equals N. davidi, or transfer the result to another pesticide. This DOI was one of the frozen census unmatched title records and is now a documented decision. |
Effects of diflubenzuron on shrimp (Neocaridina palmata) in freshwater systems dominated by submerged plant (Ceratophyllum demersum)
2023; high priority; full text needed
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Adds a direct N. palmata insect-growth-regulator study while preventing a sensitive molting-linked proxy from becoming a tank dose or product rule. |
Crossref and the official open-access Chemosphere record authenticate this CC BY article. The indexed abstract reports nominal diflubenzuron treatments of 0, 0.74, 2.222, 6.667, 20 and 60 micrograms/L in 18 indoor systems divided among six groups, with three systems per group, Neocaridina palmata and Ceratophyllum demersum. Preserve only the reported highest NOEC of 0.167 and LOEC of 0.536 micrograms/L based on chitobiase activity or immunoreactive content, plus the statement that a subcellular LOEC was below 0.107 micrograms/L. Indexed full-page text says measured day-zero concentrations were 9 to 41 percent below nominal and half-lives ranged from 2.8 to 4.0 days. A 2026-08-12 retrieval audit found that ScienceDirect required human verification, the official Elsevier API returned only minimized metadata without authorized full text, Crossref confirmed the CC BY version link, and OpenAlex located no repository full text. The complete article and 3 MB supplementary document were not retrieved. Full text and supplements are required to verify shrimp stock identity, acclimation, animals per system, allocation, independence, measured concentration table, time-weighted-average derivation, controls, feeding, water chemistry, endpoint sampling and denominators, antibody validation, statistical model, comparison pairs, multiplicity and uncertainty. Chitobiase is a molting-linked production proxy, not a direct ecdysis-failure count, mortality threshold or N. davidi care target. Do not infer a safe product concentration, a household contamination trigger or equivalence with the related tebufenpyrad or thiacloprid systems. This DOI was one of the frozen census unmatched title records and is now a documented decision. |
Environmental exposure and ecological risk of perfluorinated substances (PFASs) in the Shaying River Basin, China
2023; high priority; full text needed
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Adds a previously missing direct 28-day PFBA and PFBS mortality lead while preventing regulatory species-sensitivity values from becoming aquarium water limits. |
The official Chemosphere record, PubMed PMID 37478992 and EPA HERO record 11412305 authenticate a field-occurrence, eight-species toxicity and aquatic-life-criteria study. Indexed publisher tables report Neocaridina denticulata 28-day mortality NOEC values of 0.420 g/L PFBA and 0.070 g/L PFBS, species mean acute values of 7.866 and 0.713 g/L, and acute-to-chronic ratios of 18.73 and 10.19. These are chemical-specific test results, not measurements of ordinary aquarium exposure. The complete article and supplement remain required to verify shrimp source and identity, size and life stage, acclimation, vessel number and volume, animals per vessel, independent replication, allocation, controls, PFAS salt and purity, concentration series, renewal, analytical verification, water chemistry, feeding, mortality checks and definition, censoring, NOEC test and multiplicity, raw denominators, uncertainty and whether each species test was newly run or imported. ScienceDirect presents human verification and no lawful repository manuscript was located in this access pass. Do not convert the NOECs, species mean acute values, acute-to-chronic ratios, predicted no-effect concentrations or aquatic-life criteria into an N. davidi home threshold, product-safety claim, treatment trigger or permission to expose animals. This title does not contain Neocaridina and therefore expands the priority queue without changing the frozen 198-title DOI census. |
Toxic Effects of Ammonia on the Freshwater Shrimp Neocaridina denticulata
2007; high priority; full text needed
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Identifies the strongest located chronic ammonia lead while preventing an abstract-level maximum acceptable concentration from becoming an aquarium target or intervention threshold. |
The official National Taiwan University record identifies a 79-page 2007 Fisheries Science MSc thesis, but its 623.1 KB PDF is restricted and requires authorization. Preserve only what the detailed Chinese abstract reports: acute 24-, 48-, 72- and 96-hour total-ammonia LC50 values of 109.84, 76.60, 53.15 and 37.82 mg N/L for 4.42 +/- 0.28 mm shrimp and 332.95, 266.12, 207.97 and 171.97 mg N/L for 9.22 +/- 0.41 mm shrimp; chronic nominal total-ammonia groups of 0, 0.38, 1.14 and 3.80 mg N/L; poorer growth above 1.14 mg N/L; lower female ovigerous rate, hatchling count and male appendix-masculina index above 1.14 mg N/L; no ovigerous females plus altered feeding and oxygen consumption at 3.80 mg N/L; and an abstract-labeled maximum acceptable concentration of 0.38 to 1.14 mg N/L total ammonia-N and 0.0013 to 0.0038 mg N/L NH3. Full text is required to verify stock identity, taxonomic determination, vessel number and volume, replication, animals per vessel, chronic duration, schedules and endpoint denominators, water pH, temperature and hardness, ammonia preparation and renewal, analytical verification and decay, unionized-ammonia calculation inputs, feed and intake, allocation, mortality and censoring, exact tests, uncertainty and how the maximum acceptable concentration was derived. Do not use the abstract range as a care threshold, assume N. denticulata is interchangeable with aquarium N. davidi, or treat the reported acute values as chronic limits. This non-DOI source expands the priority queue but does not change the frozen 198-title DOI census. |
Estudos do comportamento do camarão Neocaridina davidi var red quando exposto a diferentes espetros de luz
2018; high priority; method reviewed
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Adds the closest located intact-animal light-colour comparison while preventing a short location-count signal from becoming a red-light recommendation, blue-light warning or welfare claim. |
Intake complete from the open 102-page University of Lisbon dissertation after extracted-text review and visual inspection of the complete methods, results, discussion, conclusion and statistical appendices. Preserve two trials using 15-shrimp groups in four 15-litre aquaria, left-side illumination over one of two black-stone Petri dishes, counts every five minutes, relative lamp spectra and reported lit-dish means ordered red, green, white and blue. Trial one replaced the groups weekly but kept one colour on each aquarium, completely confounding colour with tank. Trial two rotated lamps every five days across four fixed groups, but lamp sequence, washout, carryover handling, model formula, response family and random effects were not reported. Preserve the abstract-method conflict of n = 120 versus 240 in trial one, the English-abstract typo of n = 6 versus 60 in trial two, and the stated 20:00 to 08:00 light period that does not overlap the stated 09:00 to 19:00 observations. Irradiance or illuminance was neither measured nor equalized, the observer was not reported blind, coloured illumination can alter visibility of red shrimp, and no welfare, growth or reproductive endpoint was tested. Do not recommend red light, warn against blue light, infer colour vision, specify a spectrum or photoperiod, or propose a light trap. This non-DOI repository source expands the queue and evidence corpus but does not change the frozen 198-title DOI census. |
Ageing the unageable: investigating novel methods of ageing crustaceans
2020; high priority; method reviewed
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Directly tests whether bounded-age aquarium cohorts support molecular age estimation while preventing an assumed lifespan, destructive cross-sectional assay or failed research marker from becoming a consumer age test. |
Intake complete from the open 214-page University of East Anglia doctoral thesis after extracted-text review and visual inspection of all relevant pages in chapters 2, 4 and 6. Preserve two separate analytical-method studies: a 100-sample global-methylation ELISA comparison at 7, 40, 75 and 120 days after fertilization, and a 151-sample mitochondrial-sequencing study across 7 to 210 days, with 128 empirical samples passing sequencing and mapping quality control and 127 retained after one influential 40-day animal was removed. Preserve 5 to 7 berried mothers per cohort tank, fertilization windows up to eight days, age as a maximum rather than exact value, one selected cohort tank per sampled age, one mother for all egg samples, 50 percent tap plus 50 percent reverse-osmosis water, 22 to 24 C and ad libitum fish wafers. The ELISA conclusion depended on recalibration after only 30 of 100 raw readings fell within the supplied standard curves and before-recalibration inter-plate variation was 61.7 percent. The mitochondrial analysis found no age-group difference across approximately 6 kb after outlier removal, but only 5 of 9 sampled 210-day animals passed quality control, probable nuclear mitochondrial copies affected approximately 9.5 kb and technical replicate counts agreed 71 percent of the time. Do not infer a 13-month lifespan, hatch-to-death survival, exact age from appearance, a commercial age test, oxidative-damage diagnosis, inheritance pattern or named-line marker. The approximate lifespan was cited from another source, not measured. This non-DOI repository source expands the queue and evidence corpus but does not change the frozen 198-title DOI census. |
Females weigh more: evaluation of Neocaridina davidi growth and use of the alternative food Biomphalaria glabrata snail
2023; high priority; method reviewed
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Directly tests a tempting pest-to-food reuse claim while separating one short growth comparison from nutritional completeness, food safety and responsible snail control. |
Intake complete from the open 21-page UNESP repository PDF after extracted-text and every-page visual review. Preserve 42 one-week-old juveniles randomized individually across pellet-only, Biomphalaria glabrata mantle-only and equal-offering groups, excess daily feeding, 56 days, 600 mL beakers, Java moss, daily partial water changes and periodic biofilm removal. The analysis detected no feeding-treatment main effect on mass gain, but this was not an equivalence or noninferiority design and intake, leftovers, leaching, feed conversion and snail composition were not measured. Preserve that the snails were raised on the comparator pellet, 42 starters became 40 analyzed shrimp despite reported 100 percent survival, abstract and results swap the snail-only and mixed sex counts, one mixed-female table value is impossible relative to final mass and the plot, and no diet-by-sex interaction, power calculation or raw data was reported. Snail identity and infection status were not verified. Do not infer a complete diet, formulated-feed replacement, safe carcass reuse, humane preparation method, pathogen safety or infestation-control recommendation. This non-DOI repository source expands the queue and evidence corpus but does not change the frozen 198-title DOI census. |
Autophagy and apoptosis in starved and re-fed Neocaridina davidi (Crustacea, Malacostraca) midgut
2019; high priority; method reviewed
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Adds direct adult midgut evidence while separating tissue responses to prolonged food deprivation from a fasting schedule, survival threshold, re-feeding recipe, visible diagnosis and health benefit. |
Intake complete from the lawful 39-page university-repository accepted manuscript after extracted-text and every-page visual review. Preserve individually isolated 250 mL containers, algae suppression, daily excrement and exuviae removal, 7-, 14- and 21-day deprivation groups, 4-, 7- and 14-day re-feeding observations, TEM and three confocal methods. Preserve that non-starved controls came from the authors 2016 paper rather than a concurrent group, so this linked research sequence is not independent replication. Most 21-day animals reportedly died without counts, timing or survival analysis, while Table 1 still lists ten TEM specimens for each post-21-day re-feeding row. Table 1 animal counts conflict with n = 5 in Tables 2 and 3, animal-to-cell mapping is unresolved, comparison pairs and multiplicity are unspecified, and Table 3 contains impossible zero means with positive standard deviations for nonnegative percentages. Food amount, frequency and intake during re-feeding were not reported. No supplement or underlying dataset was located. Do not infer a routine fast, safe missed-feeding duration, recovery ration, biofilm adequacy, visible diagnosis, whole-animal recovery or health benefit. |
Locomotor behavior of Neocaridina palmata: a study with leachates from UV-weathered microplastics
2021; high priority; method reviewed
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Adds direct N. palmata behaviour evidence while separating a bacterial leachate response from particle exposure, a consistent shrimp locomotor effect, overall harm and household-plastic safety. |
Intake complete from the open 23-page article and official six-page supplementary DOCX. Preserve four separate material-by-weathering experiments, five concentrations, eight one-shrimp vessels per concentration, 14-day repeated observations, particle-filtered extracts and a separate three-run bacterial assay. Preserve that duplicate 24-hour leachates were combined into one 5000-fold concentrated extract per material and weathering condition, so the shrimp vessels did not independently replicate chemical preparation. The source-plastic-equivalent unit is not a measured compound concentration, particle density or aquarium dose. The two locomotor endpoints had high individual variation, few isolated differences and no consistent concentration-response. Do not infer safety, absence of harm, a named chemical culprit, natural-weathering equivalence, N. davidi response or a rule for recycled, biodegradable or household plastics. The sodium-chloride control was lethally high and excluded from the 896-trajectory analysis. The study did not measure growth, reproduction, histology or chronic aquarium exposure. Four official raw XLSX workbooks exist but were not audited in this intake because the required workbook runtime was unavailable. |
Postembryonic development and differentiation of the midgut in the freshwater shrimp Neocaridina davidi (Crustacea, Malacostraca, Decapoda) larvae
2021; high priority; full text needed
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Could improve the anatomy and early-development map, but the abstract and public peer-review history cannot resolve the final staging definitions, microscopy units, denominators or statistical analysis needed for care guidance. |
Keep full text needed after checking the official publisher record, PubMed PMID 33074574, institutional pages, scholarly indexes and all six public peer-review artifacts. The reviews authenticate a detailed ultrastructural study comparing zoea I and zoea III with microtomography, light microscopy, transmission electron microscopy, histochemistry, phosphohistone H3 labeling and a TUNEL apoptosis assay. The author response says zoea I were non-feeding and lecithotrophic while zoea III were feeding, but it does not expose the final spawning and hatching definitions, specimen counts, allocation across methods, independent microscopy units, field selection, TUNEL controls, denominators, comparison pairs or statistical results. Reviewers required correction of a duplicated image, R-cell versus E-cell terminology, cell labels, table headlines, histochemical interpretation and apoptosis-versus-necrosis wording. The response also says endocrine-cell identity could not be established because peptide-appropriate histochemistry was not performed. These revisions improve the audit trail but do not substitute for the final 18-page article, supplements or data. Do not use the abstract or reviews to overturn direct-development descriptions, define a home feeding stage, diagnose midgut health, infer probiotic effects, predict molt timing or establish maturity. Retrieve and audit the complete article, supplements and underlying image or measurement data through lawful library access or a corresponding-author request. |
Particle shape does not affect ingestion and egestion of microplastics by the freshwater shrimp Neocaridina palmata
2021; high priority; method reviewed
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Adds direct particle-ingestion and short egestion observations while separating particle recovery from toxicity, clearance, tissue transfer, shape causation and a household-plastic safety claim. |
Intake complete from the open nine-page article and official five-page supplementary DOCX. Preserve one independent run, eight one-shrimp 500 mL vessels per treatment, 24-hour exposures, seven to eight surviving replicates, a separate food comparison and separate immediate versus four-hour groups. Preserve that the supplement contains group means and standard deviations rather than animal-level data; its only actual-concentration example delivered 13 plus or minus 3 fragments versus 10 nominal per vessel; immediate and four-hour particle means came from different highly variable animals; bead and fragment shape comparisons also changed polymer, size distribution, suspension preparation and detection; fragments at or below 5 micrometres were omitted; and post-hoc Grubb outlier identities and corrected results were not disclosed. The study supports concentration-associated recovery in rinsed whole-shrimp lysates, recovery of particles in excretions and no detected food effect under this protocol. Do not infer complete clearance, a precise egestion fraction, gut-to-tissue transfer, shape independence, selective feeding, chronic harm, absence of harm, a safe aquarium concentration or that an unnamed household plastic caused exposure. Three deaths in different treatments were not a survival experiment or a demonstrated particle-toxicity mechanism. The purchased White Pearl N. palmata identity was not independently diagnosed, and N. palmata results are not automatically N. davidi results. |
First record and DNA barcodes of the aquarium shrimp, Neocaridina davidi, in Central Europe from thermally polluted River Oder canal, Poland
2018; high priority; method reviewed
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Adds a strongly identified repeated field occurrence while separating a heated-canal record from cold tolerance, establishment certainty, density, aquarium origin and environmental-impact claims. |
Intake complete from the full five-page open article and official metadata. Preserve 15 field animals collected qualitatively from submerged vegetation in July 2003, November 2013 and June 2017; only the 2017 sample had a reported one-square-metre area and approximately 15-minute effort. Preserve six amateur-aquarium comparators, 21 morphologically examined animals, one ovigerous field female from 2013, 13 newly generated 610-base-pair COI sequences, seven field and six aquarium accessions, and one shared haplotype across those new sequences. Also preserve the heated 4.5-km canal, reported 27 to 29 C June-July temperature and 15 C November temperature, formaldehyde preservation of the 2003 sample, absence of winter temperature or sampling, and the authors explicit alternative of repeated yearly introductions. Three positive years spanning more than a decade support repeated occurrence and possible persistence, not a measured continuous population, direct observation of reproduction, cold tolerance, winter survival, abundance estimate or release trace. One shared mitochondrial haplotype does not identify the aquarium source, seller, release event, nuclear ancestry or direction of transfer. Do not convert secondary discussion values into a 27 to 28 C breeding optimum, interpret low qualitative catch as density, claim no ecological effect from no detected threat, or treat a thermally polluted canal as evidence that outdoor release is safe. |
The population dynamics of some freshwater carideans (Crustacea: Decapoda) in Hong Kong, with special reference to Neocaridina serrata (Atyidae)
1985; high priority; method reviewed
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Exposes a historical-name false positive and prevents field correlations, inferred lifespan, maturity timing and clutch results from a Caridina population becoming Neocaridina care rules. |
Taxonomic screening complete from the official 1985 publisher record and the complete 1999 revision at https://doi.org/10.1080/002229399299789. Dudgeon reported a 15-month Lam Tsuen River field study under the name Neocaridina serrata, but Cai and Ng later examined Lam Tsuen material, including later Dudgeon-collected lots, and identified that population as Caridina cantonensis. They also explained that neither C. cantonensis nor C. serrata belongs in Neocaridina because the diagnostic male first pleopod condition differs. The population article remains subscription-only, so its sampling gear, dates, effort, animal counts, cohort model, exact tests and uncertainty were not audited from complete text. That access gap does not restore Neocaridina relevance after the specimen-based taxonomic revision. Do not transfer its seasonal abundance correlations, above-20-C ovigerous observation, presumed fourteenth molt, approximately 12-month inferred maximum longevity, seventh-month reproduction or clutch-size result to N. davidi, captive aquaria, a temperature optimum or a universal calendar. Keep the original title searchable, classify the census hit as a method-reviewed taxonomic exclusion and cite the later revision when explaining the correction. |
De novo assembly transcriptome analysis reveals the genes associated with body color formation in the freshwater ornamental shrimps Neocaridina denticulate sinensis
2022; high priority; full text needed
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Adds a public nine-library red, blue and chocolate cephalothorax transcriptome while separating expression associations and mined SSRs from inherited colour genes, named trade lines and breeding forecasts. |
Complete methods and supplements are still required through a library or author request. PubMed and official Elsevier metadata authenticate the closed article. The publisher abstract and preview report 1.5 to 2 cm adults from one Xiamen supplier; red, blue and chocolate phenotypes selected from forward-cross and backcross progeny and then bred within phenotype for five generations; nine cephalothorax cDNA libraries, three per phenotype; Illumina HiSeq 4000 sequencing; 75,022 assembled unigenes; 829, 554 and 3,194 differentially expressed unigenes in the three pairwise comparisons; and 6,328 mined SSRs. Official ENA project PRJNA382065 independently confirms nine PolyA paired-end runs, sample aliases RD_1 to RD_3, BL_1 to BL_3 and CH_1 to CH_3, adult cephalothorax metadata, a 2018-12-25 collection date and Xiamen location. The archive does not state the shrimp count or pooling behind each library, sex, family and parental contribution, tank allocation or independence, and the accessible article preview does not expose the complete culture conditions, phenotype measurement, RNA sampling, differential-expression thresholds, multiplicity handling, qPCR validation units or supplements. Nine libraries are not automatically nine animals or nine independent tanks. The phenotype labels are not evidence for Blue Dream, Bloody Mary, Sunkist, Chocolate or another named seller line. Pairwise expression differences in mixed cephalothorax tissue do not establish causal DNA variants, dominance, segregation, inheritance, a cross outcome or a breeding target. The 6,328 SSR sequences are mined candidates, not validated polymorphic markers or consumer line tests. Retrieve the complete article and supplements before evidence intake, establish the experimental and family units, and determine whether PRJNA382065 contains any reused 2018 skin libraries outside the nine-study subset before combining datasets. |
Identification of Functional SSR Markers in Freshwater Ornamental Shrimps Neocaridina denticulata Using Transcriptome Sequencing
2020; high priority; method reviewed
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Adds five transcriptome-derived SSR loci from super red, chocolate and reciprocal-cross breeding material while separating sampled genotype-frequency differences from a universal colour-purity or trade-line test. |
Intake complete from the full article, official Springer record and all eleven official supplementary files. Preserve one apparent pooled HiSeq 2000 library per young super red and chocolate phenotype, 105,892 assembled unigenes, 25,355 mined SSRs, 26 selected candidates, 16 feasible amplicons and five reported polymorphic loci. Supplementary Figure S4 adds the largest eight females and males from each source strain and three families per pure or reciprocal cross group. Also preserve that the stated 50 offspring per group conflicts with Table 6 hybrid frequencies in exact eighths; no individual genotype data, missing-call denominator, family allocation, standardized colour phenotype, parent-offspring segregation, linkage, independent validation population or family-aware analysis is supplied. The article and supplements do not derive the claimed 4.31 percent single-marker or 0.0018 percent combined-marker misrecognition rates, and no held-out classifier test measured them. One pooled expression library per phenotype is not replicated differential-expression evidence. Treat the five loci as marker-development candidates within the sampled Larmax material, not causal colour genes, a purity certificate, a diversity diagnosis, a named-line genotype, a dominance chart or a cross forecast. Super red in this paper is not evidence for Painted Fire Red, Bloody Mary or any other modern seller label. |
Arsenic metabolism in a freshwater food chain: Blue-green alga (Nostoc sp.) to shrimp (Neocaridina denticulata) to carp (Cyprinus carpio)
1993; high priority; full text needed
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Could add a second direct Neocaridina arsenate experiment while separating waterborne survival and accumulation from dietary transfer, methylation, nitrogen effects and aquarium safety. |
Complete methods are required through a library or author request. Crossref publisher metadata and abstract plus the EPA HERO record authenticate a Nostoc to Neocaridina denticulata to carp sequence, survival below reported 1000, 2 and 60 mg As(V) per cubic decimetre for the three organisms, a nitrogen-associated change in Nostoc accumulation, waterborne arsenate accumulation and partial methylation, carp gut distribution, and lower total accumulation with a higher methylated fraction across dietary trophic steps. The accessible abstract distinguishes route-dependent arsenic profiles: monomethylarsenic predominated in carp gut after aqueous accumulation, while food-chain transfer produced no or only trace monomethylarsenic in the three organisms. It does not expose survival duration or definition, animal count, organism source and identity, size or sex, acclimation, vessels, replication, allocation, controls, nitrogen levels, water chemistry, renewal, measured exposure, mortality schedule, tissue and whole-body allocation, feeding duration, food biomass, consumed ration, depuration, extraction, arsenic-speciation recovery, statistics or uncertainty. This is a distinct paper from the 1992 and 1994 food-chain publications but shares authors and program logic, so it is not independent laboratory replication. Do not treat survived below 2 mg/L As(V) as a safe or chronic limit, combine aqueous and dietary routes, equate methylation with detoxification, infer universal biodilution, recommend Nostoc or contaminated biomass as food, or claim that nitrogen addition controls aquarium arsenic. Retrieve all ten pages and any supplement before evidence intake. |
Biomethylation and biotransformation of arsenic in a freshwater food chain: Green alga (Chlorella vulgaris) to shrimp (Neocaridina denticulata) to killifish (Oryzias latipes)
1994; high priority; full text needed
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Could add direct arsenic-species tolerance, aqueous accumulation, depuration and experimental food-chain evidence while separating LC50, biotransformation and biodilution from aquarium safety claims. |
Complete methods are required through a library or author request. Crossref publisher metadata and abstract plus the EPA HERO record authenticate naturally collected Neocaridina denticulata, disodium arsenate, methylarsonic acid, dimethylarsinic acid and arsenobetaine comparisons, abstract LC50 values of 1.5, 10, 40 and 150 micrograms As per millilitre, selected aqueous accumulation exposures, transfer to arsenic-free medium, excretion and a Chlorella to shrimp to killifish food-chain sequence. The accessible records do not expose LC50 duration, animal count, source locality, taxon verification, size or sex, acclimation, water chemistry, vessel, replication, allocation, renewal, analytical concentration verification, mortality schedule, concentration-response model, arsenic extraction and speciation recovery, feeding duration, food biomass, consumed ration, depuration duration, excrement collection, controls, statistical units or uncertainty. Do not convert LC50 mortality medians into safe concentrations, treat total arsenic as interchangeable with chemical species, assume methylation means detoxification, infer universal biomagnification or biodilution, use the food-chain result as a feeding recommendation, or claim that transfer to clean water guarantees complete recovery. Retrieve all nine pages and any supplement before evidence intake. |
Neocaridina denticulata: A Decapod Crustacean Model for Functional Genomics
2015; high priority; method reviewed
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Preserves the historical model-organism recommendation while preventing its secondary pH, temperature, maturity, molt, egg and disease-resistance statements from becoming universal care rules. |
Review complete from the full seven-page policy paper and official Oxford metadata. Preserve that it arose from a symposium workshop, compared Neocaridina denticulata with two crayfish candidates, reported no new animals or analyses, used no systematic search or risk-of-bias method, and made a recommendation from selected earlier publications. Treat the preliminary genome as incomplete because a complete genome was an explicit next step. Treat proposed transgenesis, reporter expression, mutant screening and targeted gene control as future possibilities at publication, not demonstrated methods. Trace the pH 6.5 to 8.0, temperature up to 30 C, preferred 22 to 25 C and pH 7.0 to 7.5, 15 to 16 day intermolt, 20 to 30 egg, approximately 30 day hatch and 4 to 6 month maturity summaries to their primary sources before reuse. Do not infer a universal care range, breeding clock, stocking density, genome-completeness figure, consumer genetic tool or general resistance to bacterial infection. |
Rapid Change of Microbiota Diversity in the Gut but Not the Hepatopancreas During Gonadal Development of the New Shrimp Model Neocaridina denticulata
2015; high priority; method reviewed
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Separates an ovarian-stage-associated 16S profile from causation, probiotic benefit, fertility improvement, disease diagnosis and a Coxiella species claim. |
Intake complete from the full author-uploaded article and official metadata. Preserve nine adult females, three per visually assigned ovarian stage; three linked tissues per female; two days of starvation; 27 individually sequenced libraries; technical PCR triplicates pooled within libraries; 97 percent OTUs; low 0.5 taxonomic confidence; relative read abundance; and public SRA accession SRR1735538. Also preserve the cross-sectional design, unclear stage and egg-carrying boundary, within-female tissue dependence, underspecified t tests, no multiplicity correction or formal beta-diversity group test, no reported contamination controls and no absolute bacterial load, isolate, function, intervention, fertility, hatch, survival or disease outcome. Do not infer that microbes cause ovarian maturation, that changing tank bacteria improves breeding, that the Coxiella-type OTU is a probiotic or pathogen, or that it identifies Coxiella burnetii. |
Identification of putative ecdysteroid and juvenile hormone pathway genes in the shrimp Neocaridina denticulata
2015; high priority; method reviewed
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Adds a foundational hormone-pathway gene catalogue while separating candidate sequence homology from hormone measurement, demonstrated gene function, molt treatment and breeding intervention. |
Intake complete from the full author-uploaded article and official publisher, PubMed and institutional metadata. Preserve draft-genome TBLASTN, reciprocal BLASTX, domain and Bayesian phylogenetic checks; selected cloned partial transcripts; public GenBank accessions; and a semiquantitative JHAMT gel with three reported individual samples per group. Also preserve the one-animal draft-genome origin, unspecified RNA-stock identity and culture history, missing sex, age and molt-stage detail, inaccessible supplementary alignments and primers, absent shade hit, no hormone concentration or protein and no live-animal manipulation or outcome. Do not infer a hormone supplement, molt diagnosis, breeding treatment, mineral rule, commercial-line marker or consumer test. |
Long-Term Exposure of the Red Cherry Shrimp Neocaridina Davidi to Diclofenac: Impact on Survival, Growth, and Reproductive Potential
2022; high priority; method reviewed
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Resolves a high-ranked census lead as a preprint duplicate of the published 2023 diclofenac experiment rather than independent replication or a new evidence record. |
Duplicate intake complete from the nineteen-page CC BY Research Square preprint and the published article identity. Preserve 22 aquaria per treatment, measured means of 0.0075, 0.1320 and 1.0730 mg/L, the nonzero control, first eggs partly developed before exposure, survivor-conditioned later endpoints, non-significant among-treatment hatchling counts and the small second-spawn abnormality samples. Do not count the SSRN or Research Square posting as an independent experiment, replication, trace threshold, household medication limit or aquarium treatment. |
A type I crustin with an inhibitory effect on proteases and strong binding capacity to chitin from Neocaridina denticulata sinensis
2025; high priority; method reviewed
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Separates an antimicrobial-peptide family name, tissue expression and recombinant-protein assays from demonstrated disease resistance, shrimp treatment or chitin-supplement benefit. |
Intake complete from the official Europe PMC full text, all eleven article figures, supplementary text and supplementary figures. Preserve three healthy individuals for the ten-tissue qPCR comparison, unclear ovary and testis sampling allocation, market stock without voucher or diagnostic identity confirmation, commonly reported n = 3 with incomplete biological-versus-technical mapping, no significant inhibition of Bacillus subtilis or Vibrio parahaemolyticus growth, selected in vitro protease inhibition, qualitative chitin and chitosan binding without an unrelated-protein control, and purified-protein 30 to 100 C pretreatment. No living-shrimp bacterial challenge, NdCrus1 manipulation, survival, pathogen-load, clearance or protection outcome was measured. Do not infer disease resistance, diagnosis, treatment, a feed or supplement, a molting aid, or an aquarium temperature rule. |
Examining the Adaptive significance of colour change in the freshwater shrimp Neocaridina davidi
2025; high priority; method reviewed
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Separates a short background-associated RGB response from inherited colour, commercial grade, health and demonstrated camouflage benefit. |
Intake complete after official preprint PDF, extracted text and all seventeen rendered-page review. The registry preserves 32 males and 32 females, seven days in white followed by seven assigned days in red, blue, green or white, no feed for all 14 days, survivor-only RGB ratios, a significant among-channel difference only in blue, about 56 percent survival in white, five barcoded colony representatives, unclear vessel allocation and replication, and unreported water chemistry, molt state and mortality timing. Do not convert matching-channel trends into inherited colour, grade enhancement, an optimal substrate, improved health, predator detection or a camouflage survival benefit. |
Relationship between ROS production, MnSOD activation and periods of fasting and re-feeding in freshwater shrimp Neocaridina davidi
2019; high priority; method reviewed
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Directly tests cell-level oxidative-stress responses after prolonged feed deprivation while showing why a starvation experiment is not a routine weekly-fast recommendation. |
Intake complete after official full-PDF, extracted-text and all nineteen rendered-page review. The registry preserves one 14-day deprivation followed by 4, 7 or 14 days of re-feeding, individually isolated 250 mL containers, deliberate algae suppression, daily excrement removal, reported ROS-positive cell means, qualitative MnSOD imaging, incomplete mapping between 24 animals and 5 to 6 duplicate assay samples, unreported allocation and blinding, omitted sex and ambiguous comparison-letter reporting. Do not convert cellular oxidative-stress markers into a survival benefit, weekly fasting schedule, missed-feeding danger, proof of adequate biofilm or a re-feeding recipe. |
Effects of Dietary Manganese Supplementation on Antioxidant Enzyme Activity in the Shrimp (Neocaridina heteropoda)
2010; high priority; method reviewed
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Tests a trace-mineral feed series while separating one formulation-specific growth and enzyme response from a universal dietary requirement, molt supplement or aquarium-water dose. |
Intake complete after official open-access PDF, extracted text and all eight rendered-page review. The registry preserves 900 wild-collected shrimp, six measured complete-diet manganese concentrations, three 50-shrimp tanks per diet, one shared recirculating-water reservoir, a 30-day duration, the highest reported growth and enzyme values at 62.69 micrograms per gram, a manganese-containing control diet, unclear biochemical sample-to-tank mapping, pooled hemolymph, conflicting water-manganese units, unresolved host identification, sex and life stage, and no reported survival, feed conversion, tissue manganese, deficiency signs, molting, reproduction or long-term safety. Do not convert the response peak into a universal requirement, finished-food supplement recipe, tank-water dose, incomplete-molt treatment or proof of better health. |
Genomic Sequence and Experimental Tractability of a New Decapod Shrimp Model, Neocaridina denticulata
2014; high priority; method reviewed
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Provides the foundational short-read Neocaridina genomic resource while showing why a core-gene recovery percentage is not whole-genome completeness, line ancestry or a consumer genetic test. |
Intake complete after official Europe PMC full-text XML and visual review of all eight official figures. The registry preserves one commercially sourced red-patched adult genome donor, 364,013,140 100-base-pair reads, an assumed approximately 3-gigabase genome, 1.284 gigabases assembled into 3,346,358 contigs, a 400-base-pair N50, 455 of 458 CEGMA core-gene hits, a 15,565-base-pair mitogenome, manual removal of three bacterial-like contigs, 3,750 cutoff-dependent Daphnia matches and a separate adult MS-222 method experiment with small duration cells and observed deaths. Do not convert 99.3 percent core-gene detection into 99.3 percent whole-genome completeness, one seller animal into population or named-line genetics, candidate sequence matches into unique function, or the anaesthesia protocol into home treatment, transport, euthanasia or a welfare guarantee. |
Microinjection-based CRISPR/Cas9 mutagenesis in the decapoda crustaceans Neocaridina heteropoda and Eriocheir sinensis
2022; high priority; method reviewed
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Demonstrates targeted embryo editing while showing why three altered survivors are not ten percent of all injected embryos, a heritable line or a commercial colour assay. |
Intake complete after publisher-preview full-text review. The registry preserves local-market animals cultured for more than six months, one-cell embryos collected four hours after spawning, a dextran-versus-Phenol-Red tracer pilot, about 0.8 nL injections containing Cas9 at 300 ng/uL and sgRNA at 200 ng/uL, three Nh-scarlet targets, 28 surviving injected embryos at the phenotype screen, two altered-eye-shape survivors, one absent-eye survivor and target-region sequence changes. The initial injected denominator, maternal-clutch contributions, tracer-table denominator and replicate basis, complete negative-control structure and systematic genotyping of phenotype-normal survivors were not reported. Do not convert 3 of 28 survivors into 10 percent of all injected embryos or into adult health, body colour, fertility, germline transmission, F1 inheritance, a stable line, named-trade-line genetics or a consumer test. |
Barcoding of ornamental freshwater shrimp, Neocaridina denticulata (De Haan, 1844) from the aquatic ornamental market in Purbalingga, Central Java, Indonesia
2023; high priority; method reviewed
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Tests whether several visible market colour labels separated into different CO1 groups while exposing why a barcode result is not a trade-line pedigree or colour-based species key. |
Intake complete after official full-PDF and eight-page image review. The registry preserves ten market sequence entries, one custom approximately 545-base-pair CO1 assay, 98.24 to 99.29 percent identity to one Chinese mitochondrial reference, reported zero distance among study entries, unknown seller and aquarium clustering, absent morphology and vouchers, missing new sequence accessions, the failed blue gel lane and sample-number mismatch, Jukes-Cantor versus Kimura-model inconsistency, limited branch support and the boundary against global trade-line identity, pedigree, purity, inheritance or identification from colour. |
Confirmed by integrative taxonomy first and unusual occurrence of the exotic shrimp Neocaridina davidi (Caridea: Atyidae) in Brazil
2024; high priority; method reviewed
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Adds a vouchered morphology-plus-four-locus South American occurrence while separating one specimen from a population, marine tolerance and a traced aquarium release. |
Intake complete after official SciELO full HTML, publisher PDF, extracted text and all nine rendered pages. The registry preserves one 6.52 mm adult female, one date, one marine coastal site, three incompletely quantified trawls, voucher CCDB 6963, COI, 16S, 18S and H3 work, a COI-only comparative phylogeny, zero reported COI distance to selected Polish and Canadian records, unreported diagnostic morphology states, no ambient salinity or animal-outcome measurements, no nearby freshwater sampling and no evidence of establishment or reproduction. The H3 value is reported as a submission identifier, and a trade release or European source remains a hypothesis. Do not convert one specimen into a Brazilian population, marine tolerance, salinity guidance, introduction route, seller lineage, impact estimate or permission to release aquarium life. |
Investigating the Expression and Function of HIF-1alpha in Neocaridina davidi During Embryo Cleavage Stage
2025; high priority; method reviewed
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Separates a direct embryo gene-knockdown result from an aquarium hypoxia test, oxygen threshold or hatch countdown. |
Intake complete after full publisher-PDF and twelve-page image review. The registry preserves the nominal 300-female and 100-male groups, 600 nL dsHIF-1alpha versus dsEGFP injections, one shared-water tank, three-shrimp embryo pools with incomplete replicate mapping, missing female and embryo outcome denominators, three selected affected image sequences, no direct dsRNA tracing, no uninjected or rescue control, paired-test and significance-threshold conflicts, and the mislabeled embryo-stage gene-expression figure. No oxygen treatment was assigned and dissolved oxygen was not measured. Do not convert the HIF name, 27 C culture history, 428-hour control image sequence or photographed arrest into an aeration rule, oxygen limit, hatch calendar, viability test or home intervention. |
First report of microsporidians in the non-native shrimp Neocaridina davidi from a temperate European stream
2022; high priority; method reviewed
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Adds the earliest direct field detection, host-identification and molecular-sampling boundary for Enterocytozoon hepatopenaei and an unresolved microsporidian isolate in N. davidi. |
Intake complete after official open-access PDF, extracted-text and all six rendered-page review. The registry preserves one site and date, 75 ethanol-preserved adults, PCR from muscle tissue, host CO1 confirmation in only three randomly selected shrimp, four microsporidian-positive animals, two of three E. hepatopenaei-like sequences suitable for phylogeny, one unresolved isolate, and unreported extraction blanks, PCR negative controls and product lengths. No histology, lesions, clinical signs, survival, transmission or treatment endpoint was reported. Do not convert the four of 75 field result into aquarium or trade prevalence, visible diagnosis, disease severity, growth suppression, pathogenicity, treatment, zoonotic risk, organism origin or proof that the shrimp introduced either organism. |
Paradise under threat: the successful invasion of the freshwater shrimp Neocaridina davidi (Bouvier, 1904) and its microsporidian parasites on La Reunion Island
2025; high priority; method reviewed
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Adds a second direct field sample that links responsible no-release guidance to measured host and parasite occurrence without inventing prevalence, clinical disease or an introduction route. |
Intake complete after official repository PDF, extracted text and all eighteen rendered-page review. The registry preserves one date, three sites in two catchments, 40 shrimp per site, five of 120 molecular positives representing four reported sequence types, zero of 40 at one site, one host CO1 haplotype, no epibiont analysis, no native-shrimp parasite test, no histology, lesions, clinical signs, survival, experimental transmission, pathogenicity or treatment endpoint, and a Table 2 conflict that lists 16 immatures while reporting two positives as 2 of 14. Do not convert occurrence into aquarium or trade prevalence, proof of absence, disease severity, parasite origin, a single introduction, seller lineage, movement between catchments, proof that N. davidi introduced an organism or a treatment. |
Cell Death in the Epithelia of the Intestine and Hepatopancreas in Neocaridina heteropoda (Crustacea, Malacostraca)
2016; high priority; method reviewed
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Provides a direct adult tissue baseline showing why autophagy, apoptosis, necrosis and mitochondrial markers require method and comparison context before anyone calls them disease or treatment targets. |
Intake complete after official open-access PDF, extracted text and all twenty rendered-page review. The registry preserves no assigned external stressor, local-breeder adults maintained at 24 C, pH 7 and 15 degrees German hardness, method-specific specimen groups, only three adults behind quantitative autophagy and necrosis cell counts, an unstated TUNEL animal denominator, an arbitrary fluorescence threshold, no host-identification or health-screening method, no sex-stratified results, and a Table 1 row that prints 938 autophagic cells from 231 total where 40.26 percent implies 93. Do not convert cellular turnover into a visible diagnosis, pathogen, cause of colony decline, healthy baseline percentage, welfare state, treatment target, aquarium optimum or safe exposure. |
Omnivorous shrimp Neocaridina denticulata sinensis enhances the growth of submerged macrophyte Vallisneria denseserrulata
2019; high priority; method reviewed
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Directly separates a measured reduction in leaf periphyton from plant growth, water-column algae, nutrients and physical export. |
Intake complete after full PDF and page-image review. The registry preserves four mesocosms per treatment, eight shrimp per 75-litre tank, one plant species, 119 days, naturally varying 17 to 30 C water, repeated leaf removal, no pheophytin correction, a water-column chlorophyll unit conflict and the boundary against an aquarium cleaning rate, stocking formula or release-based restoration advice. |
Freshwater shrimp (Neocaridina denticulata) as a nature-based restoration tool for macrophyte recovery and improved water quality in eutrophic ponds
2025; high priority; full text needed
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Could test filamentous-algae consumption, macrophyte recovery and water-quality outcomes across feeding trials, microcosms and field evaluation while defining the boundary against aquarium cleanup and animal introduction. |
Full methods still needed. The official publisher abstract reports feeding trials, controlled microcosms, long-term field evaluation, reduced filamentous algae, improved clarity, an 11-fold macrophyte-abundance result and a self-sustaining shrimp population. The publisher page and official metadata do not expose animal and vessel counts, treatment allocation, controls, shrimp density, endpoint definitions, intervention timing, field comparators, replication, analysis units or the numerical water-quality results here. OpenAlex reports no repository full text or PDF. Do not convert the abstract into a home algae list, stocking rate, water-quality promise or instruction to introduce or release shrimp. Retrieve the article and supplements through the publisher, a library or the corresponding author. |
Development and testing of a sedation protocol for Neocaridina davidi
2024; high priority; method reviewed
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Could improve humane imaging, measurement and specimen handling while defining a clear research-only boundary. |
Intake complete. The registry now preserves missing vehicle control, short recovery follow-up and the boundary against home use. |
Post-embryonic tail development through molting of the freshwater shrimp Neocaridina denticulata
2025; high priority; method reviewed
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Clarifies the first-molt tail transition and separates developmental anatomy from a home molt clock or visible diagnosis. |
Intake complete. The registry preserves method-specific sample sizes, probable microscope-temperature acceleration, invasive live imaging, possible injury artifacts and the boundary against adult timing or mineral advice. |
Ectoparasites in Ornamental Shrimps Neocaridina denticulata and Neocaridina palmata from Purbalingga Fish Market Aquariums
2025; high priority; method reviewed
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Turns a dramatic market-sample percentage into a practical lesson about clustered sampling, organism identification and the difference between detection and measured disease. |
Intake complete. The registry preserves the one-seller sampling frame, shared aquarium history, missing host and organism confirmation, unreported tank clustering and the absence of any measured harm or treatment endpoint. |
Experimental measurement of enzyme activity during initial crustacean growth using the ornamental shrimp, Neocaridina denticulata, as a model and changes in activity with growth
2025; high priority; method reviewed
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Adds known-age size observations while showing why one pooled rearing tank and whole-body enzyme pools cannot define adulthood, cold tolerance or a stage-specific diet. |
Intake complete. The registry preserves destructive cross-sectional sampling, the shared tank, unknown survival denominator, unassigned temperature, whole-body pooling, incomplete statistical reporting and unclear 75-day adult identity. |
Comparative transcriptomic analysis primarily explores the molecular mechanism of compound eye formation in Neocaridina denticulata sinensis
2024; high priority; method reviewed
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Separates visible embryo eye stages from a universal hatch countdown, viability claim or reason to alter the aquarium. |
Intake complete. The registry preserves three roughly 50-embryo pools per stage, unknown female and brood contributions, missing incubation and time-to-hatch data, whole-embryo expression associations and the absence of functional or hatching endpoints. |
The occurrence process of chromatophores in three body color strains of the ornamental shrimp Neocaridina denticulata sinensis
2022; high priority; method reviewed
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Shows when pigment-cell types first appeared in three laboratory strains while separating microscopy stages from a universal juvenile grading clock. |
Intake complete. The registry preserves six-individual observations, one tank per strain, unknown family contributions, in-vitro egg culture, incomplete statistical reporting and the boundaries against diet, inheritance, named-line and adult-grade claims. |
Genome-wide identification of the crustacyanin gene family in Neocaridina denticulata sinensis based on comparative genomics and localization analysis
2025; high priority; method reviewed
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Explains what a pigment-binding protein family map can reveal while preventing gene count from becoming a trade-line inheritance chart, grading claim or colour-food recommendation. |
Intake complete. The registry preserves one unpublished source genome, 15 tissue libraries with unresolved animal-versus-pool provenance, undefined qPCR biological units, antibody cross-recognition, missing colour phenotypes and the boundary against assigning individual-gene functions or commercial-line traits. |
Enhancing Histological Techniques for Small Crustaceans: Evaluation of Fixation, Decalcification, and Enzymatic Digestion in Neocaridina Shrimp
2025; high priority; method reviewed
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Shows why post-mortem preparation can change tissue appearance and why a laboratory slide or deceased specimen image is not automatically evidence of disease. |
Intake complete. The registry preserves five animals per processing variant, specimen-level subsampling, subjective unblinded scoring, one breeder line without species identification, laboratory chemical hazards and the boundary against live-health, treatment or home euthanasia claims. |
Annoying noise: effect of anthropogenic underwater noise on the movement and feeding performance in the red cherry shrimp, Neocaridina davidi
2023; high priority; method reviewed
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Directly addresses vibration and noise questions that hobbyists often infer from short behaviour observations. |
Intake complete. The registry now preserves the single-tank design, unmeasured particle motion and equipment-transfer limits. |
Impacts of sublethal concentrations of 17 alpha-ethinylestradiol on growth, reproductive performance and survival in red cherry shrimp during consecutive spawnings
2023; high priority; method reviewed
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Provides long reproductive endpoints for a defined water contaminant and may improve chemical-exposure claim boundaries. |
Intake complete. The registry preserves nominal-only exposure, the unmatched solvent control, conflicting ethanol reporting, low control survival, artificial egg incubation and the boundary against a beneficial low dose. |
Long-Term Exposure of the Red Cherry Shrimp Neocaridina davidi to Diclofenac: Impact on Survival, Growth, and Reproductive Potential
2023; high priority; method reviewed
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May add a long-duration direct toxicant design with survival, growth and reproductive endpoints. |
Intake complete. The registry preserves aquarium replication, measured concentrations, endpoint denominators and the boundary against a household threshold. |
The lifecycle of Neocaridina denticulata and N. palmata in aquariums
2020; high priority; method reviewed
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Tests a widely repeated 75-day maturity claim against the source tanks, offspring denominators, life-stage language and water-measurement record. |
Intake complete. The registry preserves one initial aquarium per market label, unclear female and offspring units, absent survival denominators, inconsistent dates, tables and figures, and the boundary against a 75-day maturity calendar, 27 C optimum or water-quality threshold. |
Breeding and Life Cycle of Neocaridina denticulata sinensis (Kemp, 1918)
2013; high priority; method reviewed
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Adds direct breeding and known-cohort observations while showing why one selected shared cohort cannot create a universal maturity calendar or salt-treatment protocol. |
Intake complete after official full-HTML, repository-PDF and page-image review. The registry preserves one breeding tank, one shared 30-offspring life-cycle tank, selection of only healthy one-week-old offspring, missing survival and maturity denominators, unexplained extra points in the brood-mass graph, its 0.3589 figure versus 0.9587 and 0.959 text conflict, and the absence of an assigned salinity treatment, untreated control, dose-duration, host-safety endpoint or recurrence follow-up. Do not convert the observed 15-day incubation, 75-day maturity, 27 C water, 40 percent protein feed or 5 to 10 ppt salinity claim into a universal calendar, optimum, feeding recipe or treatment. |
Estimation of the size of sexual maturity in reproducers of Neocaridina heteropoda under laboratory conditions
2020; high priority; method reviewed
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Shows why selected adult broodstock and an incompletely documented F1 cannot validate a universal age or size of sexual maturity. |
Intake complete. The registry preserves six nominal 2-litre aquaria, selected adults, undefined F1 hatch and age assignment, conflicting maturity-week summaries, incomplete egg and water reporting, mortality-driven density changes and statistical inconsistencies. |
Functional Analysis of NdBCO-like4 Gene in Pigmentation of Neocaridina denticulata sinensis
2025; high priority; method reviewed
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Adds a direct functional pigment-gene experiment while testing whether one associated SNP can identify commercial colour lines or predict crosses. |
Intake complete. The registry preserves the five paired maternal RNA-interference units, artificial removed-embryo exposure, unknown culture-tank replication, contradictory significance and SNP descriptions, unreconciled genotype totals, wild-versus-transparent label switch and the boundary against a named-line genetic test. |
Role Analysis of the scarb1 Gene in the Pigmentation of Neocaridina denticulata sinensis
2025; high priority; method reviewed
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Tests a carotenoid-transport candidate and a yellow-population SNP while showing why a strong sampled association is not a consumer test for Sunkist or another named line. |
Intake complete after full XML review. The registry preserves five maternal split-clutch RNAi units, failed later-stage silencing, unreported statistical methods for most comparisons, 345 reported of 384 planned genotypes per population and the absence of crosses, causation, independent-line validation or individual prediction. |
Identification of a new ABCB6 gene correlated with economic trait for pigmentation of cherry shrimp (Neocaridina denticulata)
2026; high priority; full text needed
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Could add another functional pigment-transport candidate while testing whether an economic-trait title is being overextended into commercial-line genetics. |
Full methods still needed. The publisher abstract reports red, yellow, blue and wild laboratory strains, expression profiling, in situ hybridization and RNA interference, but accessible records do not expose animal and maternal units, strain and tank replication, controls, allocation, phenotype scoring, analysis units or full statistical methods. Do not convert the abstract into a named-line marker, inheritance model, colour-food claim or breeding test. Retrieve the article and supplements through a library or corresponding-author request. |
Crustacyanins as regulators of functional complementation, cuticle development, lipid metabolism, pigmentation, and immunity in shrimp
2026; high priority; full text needed
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Could clarify functional overlap among 24 crustacyanin-family genes while preventing multi-omics pathway labels from becoming named-line genetics, colour recipes, molting advice or disease-resistance claims. |
Full methods still needed. The official publisher preview reports 24 NdCRCN genes, separate RNA-interference targeting of the highest-expressed A and C subunits, transcriptomic and proteomic comparisons, compensatory expression among family members, and anti-NdCRCN co-immunoprecipitation followed by LC-MS/MS. Accessible records do not expose shrimp and vessel counts, stock identity, sex or molt state, dsRNA and injection controls, allocation, knockdown denominators, library pooling, phenotype measurements, co-immunoprecipitation controls, endpoint-specific replication or full statistical methods. The preview reports pathway and interacting-protein results, not named trade-line crosses, standardized visible-colour change, carotenoid chemistry, whole-animal molt outcomes, bacterial challenge, survival or disease protection. A retained crustacyanin PDF was verified page by page as the distinct 2025 BMC Genomics article at DOI 10.1186/s12864-025-12371-6 and cannot satisfy this 2026 Aquaculture lead. Do not convert gene-family membership, pathway enrichment, compensatory expression or protein interaction into a breeding marker, colour-food recipe, molt intervention, immune supplement or disease-resistance claim. Retrieve the article and supplements through a library or corresponding-author request. |
Mechanistic insights into novel triclosan target pathways in Neocaridina denticulata revealed by multi-omics integration
2026; high priority; full text needed
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Could add a direct acute household-antimicrobial exposure with measured body accumulation, molecular pathways and biochemical validation. |
Full methods still needed. PubMed, Crossref and the publisher abstract report 24-hour nominal exposures of 1, 5 and 10 micrograms per litre with whole-body multi-omics and biochemical endpoints, but the accessible material does not expose animal and vessel counts, replication, allocation, solvent control, measured water concentrations, omics pooling, endpoint-specific denominators or analysis units. Do not convert the abstract into an aquarium threshold, product warning, clinical sign or decontamination protocol. Retrieve the article and supplements through a library or corresponding-author request. |
Genes are differentially expressed at transcriptional level of Neocaridina denticulata following short-term exposure to nonylphenol
2011; high priority; method reviewed
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Adds direct chemical-response evidence while teaching why a pooled transcript change is not automatically protein change, whole-animal harm, a household diagnosis or a safe concentration. |
Intake complete from PubMed and indexed author-personal-copy article content. The registry preserves one-day nominal exposures, pooled whole-animal RNA, no reported independent exposure-vessel replication or random allocation, no multiplicity correction, the abstract-versus-results conflict over six or five responsive transcripts at 0.01 mg/L, the PBS-versus-DMSO control-label conflict and the boundary against a protein, functional-harm, chronic, diagnostic, threshold or biomarker-protocol claim. |
Development of Ecotoxicogenomic Biomarkers on the Freshwater Shrimp (Neocaridina denticulate) Following Short-Term Exposure to Dipropyl Phthalate
2013; high priority; method reviewed
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Adds an open bilingual molecular exposure paper and a concrete example of why transcript direction, pooling, solvent controls and treatment-vessel replication must be checked beyond the abstract. |
Intake complete after full official-PDF text and 12-page rendered review. The registry preserves the two-hour exposure plus 12-hour clean-water discovery design, one-day concentration validation, pooled whole-animal RNA, unreported independent exposure-vessel replication and allocation, unmatched acetone control, nominal-only concentrations, inconsistent ten-versus-at-least-five shrimp pool wording, hidden control dispersion, no multiplicity correction, the English-abstract-versus-main-results direction conflict and the table footnote that mistakenly names nonylphenol. Do not convert selected transcript changes into protein abundance, immune competence, toxicity, survival, disease susceptibility, chronic harm, a plastic-product diagnosis, threshold or validated biomarker panel. |
Research on the Response of Neocaridina denticulate to the Toxicity of Cu2+ and Chlorpyrifos
2014; high priority; full text needed
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Identifies the acute mortality and mixture experiment that supplied the 96-hour LC50 values inherited by the 2015 copper and chlorpyrifos biomarker paper. |
The exact eight-page journal PDF and source identity are located. Its extracted text exposes a 96-hour semi-static design, three parallel beakers per nominal concentration, blank and chlorpyrifos vehicle controls, probit LC50 analysis and a copper-ion plus chlorpyrifos mixture analysis, but the embedded font drops the decisive concentration, interval and additive-index table cells. Direct rendered-page inspection is still required before intake. A 2026-08-12 retrieval audit found that the official PDF returned HTTP 418 to a direct request and an access-blocked page in a normal browser; a focused exact-title search found citations but no accessible primary copy. Keep this lead outside evidence. Do not promote the 3.44 mg/L copper-ion or 0.06 micrograms/L chlorpyrifos values repeated by the 2015 companion paper, the later 0.4 micrograms/L 24-hour chlorpyrifos citation, the reported synergy classification or the unquantified molting observation into primary table evidence, an N. davidi threshold, a safe aquarium level or a mixture prediction. |
Physiological Response of Neocaridina denticulate to the Toxicity of Cu2+ and Chlorpyrifos
2015; high priority; method reviewed
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Corrects the claim that no direct controlled Neocaridina copper exposure was located while preserving the species, water, chemical-form, biomarker and safe-level boundaries. |
Intake complete from the official full journal HTML. The registry preserves wild-source animals labelled Neocaridina denticulate without a reported identification method, three parallel beakers per concentration, nominal copper-ion and chlorpyrifos series, daily renewal, five-day fasting, unclear destructive-sample mapping to beakers, repeated concentration-by-day testing and concentration-label inconsistencies. The 96-hour LC50 values used to select treatments came from the now-identified 2014 acute predecessor, not this experiment; that predecessor remains full text needed because its extracted numerical tables are incomplete. Do not convert inherited LC50 values, muscle biomarkers or zero mortality in the selected sublethal groups into N. davidi identity, a no-effect level, safe home concentration, diagnosis or dosing instruction. |
Gene expression profiling and expression analysis of freshwater shrimp (Neocaridina denticulata denticulata) using expressed sequence tags and short-term exposure to copper
2018; high priority; method reviewed
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Adds a second direct copper transcript experiment while showing why pooled RNA, an unreported exposure unit and an internally inconsistent water record do not validate a field biomarker or safe level. |
Intake complete after full official-PDF text extraction and rendered review of all seven pages. The registry separates the ten-animal non-exposed baseline cDNA library from the three-animal pooled copper series, preserves the figure-labelled 0 to 100 micrograms per litre 24-hour dose range, the unstated time-series concentration, unreported exposure vessels and allocation, nominal-only water concentration, sole beta-actin reference, unclear biological replication, missing multiplicity model and the 28 PSU salinity statement that conflicts with the freshwater lake description. Do not convert selected pooled whole-body mRNA changes into protein abundance, toxicity, molting impairment, immune competence, diagnosis, a safe concentration or validated biomarker panel. |
Transcriptome analysis of Neocaridina denticulate sinensis under copper exposure
2021; high priority; full text needed
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Could add a cephalothorax RNA-seq copper experiment and clarify whether immune and molting pathway interpretations reproduce across tissues and better-defined biological units. |
Full methods still needed. The official publisher preview reports 20 adult shrimp per control or nominal 2.5 micromoles per litre copper-ion group for 48 hours, one described tank per group, three separately dissected cephalothorax samples per group, six RNA-seq libraries, 122,512 assembled unigenes, 219 differentially expressed genes and seven qPCR checks. The accessible material does not expose complete tank replication, water chemistry, copper verification, sample-to-animal mapping, RNA-seq contrasts, qPCR denominators, reference genes or full statistical methods. Do not convert abstract pathway enrichment or transcript direction into molting impairment, immune competence, whole-animal toxicity, a biomarker panel, diagnosis or safe aquarium concentration. Retrieve the full article and supplements through a library or corresponding-author request. |
Transcriptomic analysis of Neocaridina denticulate sinensis hepatopancreas indicates immune changes after copper exposure
2022; high priority; full text needed
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Could add a 12-hour hepatopancreas RNA-seq comparison to the same laboratory group's 48-hour cephalothorax study while testing whether tissue and time differences are being mistaken for independent replication. |
Full methods still needed. The official publisher preview reports laboratory-bred adults, 20 shrimp randomly selected per 0 or nominal 2.5 micromoles per litre copper-ion group, analytical-grade copper sulfate pentahydrate, a 12-hour endpoint, six hepatopancreas RNA-seq libraries, 23,266 assembled unigenes, 16,423 differentially expressed genes and eight qPCR checks. The accessible material does not expose the completed copper-preparation sentence, treatment-vessel count and volume, water chemistry, analytical copper verification, sample-to-animal and sample-to-tank mapping, library pooling, qPCR denominators and reference genes, full differential-expression thresholds, multiplicity handling or whether this cohort was independent of the same group's 2021 cephalothorax paper. ResearchGate offers an author-request page rather than full text. Do not convert abstract immune-pathway enrichment or transcript direction into immune competence, detoxification, copper tolerance, toxicity, disease resistance, a diagnosis, safe concentration or independent replication. Retrieve the article and supplements through a library or corresponding-author request. |
Membrane-bound trehalase enhances cadmium tolerance by regulating cell apoptosis in Neocaridina denticulata sinensis
2024; high priority; full text needed
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Tests a cadmium-responsive trehalase candidate while exposing the crucial difference between shrimp molecular responses and metal tolerance measured in recombinant bacteria. |
Full methods still needed. PubMed, the publisher abstract and the Hebei University laboratory summary report NdTre2 cloning, gill-biased expression that decreased 12 hours after cadmium stimulation, double-stranded-RNA knockdown, gill transcriptomics, selected apoptosis-pathway validation, DNA fragmentation and TUNEL/DAPI staining. The reported cadmium-tolerance assay overexpressed recombinant NdTre2 in Escherichia coli and measured bacterial survival and growth, not shrimp survival or recovery. Accessible sources do not expose shrimp and vessel counts, cadmium concentrations and preparation, water chemistry, measured water or tissue cadmium, exposure and injection controls, allocation, RNA-seq pooling, endpoint denominators, blinding, complete statistical methods or whole-animal shrimp outcomes after knockdown. ResearchGate offers an author-request page rather than full text. Do not convert the title, pathway enrichment, gill apoptosis staining, recombinant-protein binding or bacterial assay into demonstrated shrimp cadmium tolerance, immune competence, molting control, a safe concentration, diagnosis, treatment, supplement or genetic test. Retrieve the article and supplements through a library or corresponding-author request. |
Identification and functional analysis of a novel L-type lectin (NdLTL1) from Neocaridina denticulata sinensis
2025; high priority; full text needed
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Separates shrimp challenge-responsive expression from recombinant-protein binding and bacterial agglutination while preventing an in vitro assay from becoming disease-resistance or supplement advice. |
Full methods still needed. PubMed, the publisher preview and the Hebei University laboratory summary report a market source, one-week acclimation at 25 C, genome-wide mining of 179 lectin-like sequences, high cardiac NdLTL1 expression, increased gill expression after Vibrio parahaemolyticus exposure, recombinant NdLTL1 produced in Escherichia coli, binding to several bacteria and carbohydrates, calcium-dependent bacterial agglutination and in vitro bacterial-growth assays. Accessible sources do not expose challenge animal and vessel counts, independent tank replication, bacterial dose and route, controls, allocation, tissue pooling, sampling denominators, recombinant-protein and assay controls, complete replication or full statistical methods. No accessible record reports shrimp survival, disease signs, bacterial load, clearance or protection after manipulating NdLTL1. ResearchGate states that no full text is available and offers a request page. Do not convert challenge-responsive expression, binding, bacterial clumping, calcium dependence or in vitro bacterial growth into demonstrated shrimp immune competence, disease resistance, a diagnosis, treatment, probiotic, prebiotic, sugar product or calcium supplement. Retrieve the article and supplements through a library or corresponding-author request. |
RNA-seq analysis revealing the immune response of Neocaridina denticulata sinensis gill to Vibrio parahaemolyticus infection
2022; high priority; full text needed
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Adds the direct gill transcript experiment cited by the later NdLTL1 paper while separating PBS-versus-bacterial stimulation from infection severity, disease protection and treatment evidence. |
Full methods still needed. PubMed and the publisher preview report market-source animals 1.5 plus or minus 0.2 cm long, one month in a 25 C laboratory circulating-water system, a PBS control and Vibrio parahaemolyticus stimulation comparison, six gill RNA-seq libraries labelled C12_1 through C12_3 and S12_1 through S12_3, 1,358 differentially expressed genes and qPCR checks of six selected lysosome-pathway genes. Accessible material does not expose the complete bacterial preparation, challenge dose and route, animal and vessel counts, independent tank replication, allocation, whether gill libraries pooled animals, sample-to-animal and sample-to-tank mapping, qPCR denominators and reference genes, tissue-damage methods, complete statistical thresholds or multiplicity handling. No accessible record reports shrimp survival, clinical signs, bacterial load, clearance, transmission or protection. Do not convert transcript direction or pathway enrichment into natural-infection risk, disease diagnosis, pathogenicity, immune competence, disease resistance, treatment, prevention or a validated biomarker panel. Retrieve the article and supplements through a library or corresponding-author request, then compare its animals and challenge protocol with the same laboratory group's later NdLTL1 paper rather than assuming an independent replication. |
Transcriptome analysis of Neocaridina denticulate sinensis challenged by Vibrio parahemolyticus
2022; high priority; full text needed
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Adds a hepatopancreas transcript comparison while separating an injected challenge discussed in an AHPND context from evidence that the tested strain carried PirAB toxins or caused AHPND or whole-animal disease in Neocaridina. |
Full methods still needed. PubMed and the publisher preview report open-fair animals maintained in the laboratory for two years, 30 healthy shrimp approximately 1.5 plus or minus 0.5 cm long, 25 plus or minus 1 C circulating water, hepatopancreas sampling after Vibrio parahaemolyticus injection, six cDNA libraries, 23,624 genes, 21,464 differentially expressed genes and eight qPCR checks. The accessible preview cuts before the complete acclimation and bacterial-challenge methods and does not expose group allocation, bacterial dose and volume, strain characterization, injection and control details, animal and vessel counts per treatment, independent tank replication, library pooling, sample-to-animal and sample-to-tank mapping, qPCR denominators and reference genes, complete differential-expression thresholds or multiplicity handling. It reports no shrimp survival, clinical signs, bacterial load, clearance, transmission or hepatopancreas pathology. The title, abstract and keywords discuss AHPND, but the accessible record does not establish that the challenge strain carried PirAB toxins or caused AHPND in Neocaridina. Six libraries are not automatically six animals or tanks, and 21,464 changed transcripts are not 21,464 disease mechanisms. Do not infer natural aquarium infection risk, an AHPND diagnosis, pathogenicity, immune competence, disease resistance, treatment, prevention or a validated biomarker panel. Retrieve the article and supplements through a library or corresponding-author request, then establish whether this was a distinct animal cohort and challenge protocol from the same laboratory group's 2022 gill RNA-seq paper rather than calling the two independent replication. |
Exploiting the Freshwater Shrimp Neocaridina denticulata as Aquatic Invertebrate Model to Evaluate Nontargeted Pesticide Induced Toxicity by Investigating Physiologic and Biochemical Parameters
2021; high priority; method reviewed
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Adds direct movement, heartbeat and maxilliped-movement endpoints for one insecticide while teaching why animal-level videos do not repair unreplicated treatment tanks. |
Intake complete after full publisher-PDF and 24-page rendered review. The registry preserves a commercial 28.8 percent imidacloprid formulation, nominal-only exposures, no formulation-only control, apparent one-tank-per-treatment assignment, conflicting 12-versus-20 animal descriptions, fasting effects, repeated individual measurements, small whole-animal assay groups, one-tank rescue groups, active effects of acetylcholine and docking to a snail acetylcholine-binding protein rather than a shrimp receptor. Do not convert movement or cardiac effects into a home diagnosis, recovery claim, acetylcholine antidote, product rule, aquarium dose or universal threshold. |
Acute toxicity of mixture of acetaminophen and ibuprofen to Green Neon Shrimp, Neocaridina denticulate
2014; medium priority; method reviewed
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Adds a direct 96-hour juvenile mortality experiment while showing why a lower mixture LC50 is not automatically proof of synergy or an aquarium threshold. |
Intake complete after review of the author-uploaded full text and tables. The registry preserves river-source animals, 10- to 14-day-old approximately 1.5 mm juveniles, 20 shrimp per reported group, 1-litre beakers, nominal static-renewal exposures, DMSO stocks, sodium-chloride reference tests, four stated repeats, duplicate treatments, Table 2 n = 4, five mortality times and full LC10, LC50 and confidence intervals. The paper does not reconcile repeats, duplicates and n; report measured concentrations, DMSO fraction or vehicle control; define whether the 1:1 concentration is per component or summed; or add the fixed 0.5 mg/L co-exposure to unequal-series LC50 labels. Its synergy and antagonism language lacks a concentration-addition, independent-action, toxicity-unit, model-deviation or isobologram analysis, and the abstract pairs non-significance with p < 0.05. Do not convert acute nominal mortality in tiny juveniles into a safe aquarium level, chronic outcome, medication instruction, disposal rule, wastewater prediction or universal mixture rule. |
Differential immune responses of the green neon shrimp (Neocaridina denticulate) to dipropyl phthalate
2011; high priority; full text needed
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Could connect DPrP exposure to time-specific immune assays and bacterial-challenge mortality without inferring disease resistance from transcript levels alone. |
Full methods still needed. PubMed and the publisher abstract report nominal 0, 1, 5, 10 and 50 mg/L DPrP exposures, six immune parameters, Aeromonas veronii challenge and mortality on days 1, 3, 5 and 10, but the article is paywalled and the indexed request page exposes no complete methods. Animal and vessel counts, treatment replication, acetone control, exposure renewal, bacterial dose and route, challenge allocation, endpoint denominators, censoring and analysis units remain unverified. Do not convert abstract mortality percentages into an aquarium threshold, plastic-product warning, infection diagnosis, immune supplement claim or treatment protocol. Retrieve the article through a library or corresponding-author request. |
Effect of different substrates on growth and biochemical composition of the ornamental red cherry shrimp Neocaridina davidi
2022; high priority; full text needed
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Could separate substrate effects on growth from colour preference and general habitat assumptions. |
Full methods still needed. The 90-day abstract compares Vesicularia, Ceratophyllum, Cabomba and plastic net and reports growth, survival and ovigerous-female outcomes. The official 710291-byte CONICET published-version deposit is restricted, Wiley exposes only the abstract without access, and scholarly indexes locate no open copy. Aquarium replication, allocation, conditioning, surface area, feeding and analysis units remain unverified, so the percentages do not support a habitat recommendation. Request the article or study data from the corresponding author. |
Characterization of the moult cycle in Neocaridina denticulata sinensis Kemp, 1918: the moulting frequency, moulting stages, and haemolymph ecdysteroid levels
2022; high priority; full text needed
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Could replace generic molt-frequency calendars with life-stage, temperature and visible-stage observations from a direct Neocaridina study. |
Full methods still needed. The abstract reports 14 molts from hatch through first complete pleopod development at 25 C, four main periods, five premolt substages and adult male-versus-female ecdysteroid patterns. The publisher article is paywalled and no legal public manuscript was located. Animal counts, observation intervals, rearing units, independence, censoring and the exact definition of first complete pleopod development remain unverified, so the abstract does not support a home molt calendar or intervention. Request the article or study data through a library or corresponding author. |
Effect of social environment on sexual differentiation in the highly gregarious red cherry shrimp Neocaridina davidi
2019; high priority; method reviewed
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Directly tests whether adult sex composition changes juvenile phenotypic sex under one controlled protocol. |
Intake complete. The registry preserves brood-level replication, one excluded brood, pleopod-based sexing and the boundary against a universal genetic mechanism. |
Effects of dietary incorporation of Arthrospira platensis meal on growth, survival, body composition, and reproductive performance over successive spawnings
2017; high priority; method reviewed
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Measures several outcomes that hobby food claims frequently combine without a controlled denominator. |
Structured after author-uploaded full-text review. Keep fish-meal replacement, three aquarium replicates, artificial egg incubation, low control survival and the dry-formulation boundary visible. |
Leaf-litter preferences of the introduced freshwater shrimps Atyaephyra desmarestii and Neocaridina davidi
2017; high priority; full text needed
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Could replace generic botanical claims with a bounded comparison of conditioned leaf species, life stages and consumption. |
Full methods still needed. The official University of Cologne record and OAI metadata identify the accessible 174 kB file as a four-page Thesis Abstract. The German National Library catalogs a physical 63-page 2018 dissertation, and its official contents page places the leaf-litter article in chapter III on pages 34 to 50. It supplies no electronic dissertation file. OpenAlex reports no repository with full text, and its apparent University of Cologne OAI identifier 24511 is stale because the repository returns idDoesNotExist. The publisher reader stops at human verification and its machine-readable article route denies access. The abstracts report adult versus juvenile preferences, nighttime activity and a 51.0 percent leaf-litter dry-weight result normalized to body dry weight, but choice-vessel replication, conditioning duration and water, leaf mass or area normalization, position rotation, other feeding, decomposition and biofilm controls, non-consumption mass-loss correction, observation schedule, body dry-weight estimation, experimental unit and analysis remain unverified. These abstract findings do not support a leaf recommendation, aquarium ration, replacement interval, complete-diet claim or health claim. Request the 16-page article, physical dissertation or study data through a library, repository or corresponding author. |
Promotive performance of shrimp Neocaridina denticulata on Typha angustifolia leaf litter decomposition
2019; high priority; method reviewed
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Separates direct shrimp contact from fecal effects on one leaf litter and shows why decomposition, ingestion, water chemistry and shrimp nutrition are different claims. |
Intake complete after full author-uploaded article review. The registry preserves 64 four-litre treatment-by-time buckets, four units per treatment and destructive 35-day harvest, eight river-collected shrimp plus 3 g air-dried cattail litter in each shrimp unit, weekly filtered river-water replacement, no formulated-food baseline, derived rather than measured grazing shares, inconsistent Typha angustifolia versus Typha orientalis labels, conflicting animal holding descriptions, no reported random allocation or obvious sham-mesh control, and the unexplained use of repeated-measures analysis for destructive units. Reported zero mortality cannot establish dietary adequacy without intake, growth, condition, molting, reproduction or health endpoints. Do not convert litter mass loss into a cattail, catappa or alder recommendation, ration, complete-diet claim, replacement interval, home water-safety promise or shrimp-per-leaf formula. |
Effects of different aquarium feeds on growth performance and survival rate of red cherry shrimp Neocaridina denticulata
2016; high priority; method reviewed
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Direct feed comparison could improve practical feeding guidance if the products, ration and units are recoverable. |
Structured after full-method review. Keep product formulation and physical form confounded, feeding to satiation explicit and the aquarium as the experimental unit. |
The effectiveness of natural feed in enhancing color and survival rate of ornamental shrimp Neocaridina sp.
2025; high priority; method reviewed
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Directly tests common live-food and colour claims while exposing why a treatment mean is not a complete-food, biosecurity or grade recommendation. |
Intake complete after full open-PDF and page-image review. The registry preserves three aquaria per feed, an unresolved integer starting count, genus-only stock identity, unmeasured ad-libitum intake, unidentified control pellets, the conflicting four-versus-five colour scale, contradictory significance note and discussion, pooled water ranges and the boundary against a Tubifex dose, complete-food claim, colour-grade method or instruction to introduce live worms. |
Effect Of Market Vegetable Waste Fermented Feeding On The Growth Of Red Cherry Ornamental Shrimp (Neocaridina davidi)
2023; high priority; method reviewed
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Directly addresses vegetable-feeding folklore while separating one fermented preparation comparison from fresh produce, food-safety and complete-diet claims. |
Intake complete. The registry preserves three aquaria per feed, ad-libitum availability, unstandardized intake and dry matter, unresolved analysis unit, substantial handling-associated mortality and the boundary against grocery-scrap or home-fermentation recipes. |
Effects of chlordane and lindane on testosterone and vitellogenin levels in green neon shrimp Neocaridina denticulata
2004; medium priority; method reviewed
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Adds the acute-to-juvenile start of a three-paper pesticide sequence while separating mortality, biochemical proxies and later reproductive outcomes. |
Intake complete after full repository-PDF and page-image review. Preserve triplicate beaker wording alongside the absent chronic sampling denominator and beaker-to-assay mapping, the acute group-count and confidence-interval conflicts, nominal concentrations, nonspecific alkali-labile phosphate proxy, unmatched alcohol positive-control vehicle and repeated paired tests without multiplicity correction. Do not use the reported LC50 values as aquarium targets or call this an independent replication of the later male or female experiments. |
Effects of the endocrine disrupter chemicals chlordane and lindane on the male green neon shrimp Neocaridina denticulata
2004; medium priority; method reviewed
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Adds direct male hormone-associated and appendage endpoints while preventing a variable morphometric response from becoming a visible diagnosis. |
Intake complete after full repository-PDF and page-image review. Preserve three exposure beakers per group, unexplained mapping of figure n values to beakers, nominal concentrations, no reported renewal schedule, nonspecific alkali-labile phosphate proxy, unmatched alcohol positive-control vehicle and repeated paired tests without multiplicity correction. Appendage effects varied by time and concentration and reproductive competence was not tested. Treat it as a separate linked experiment, not an independent replication. |
Reproduction obstacles for the female green neon shrimp after exposure to chlordane and lindane
2006; medium priority; method reviewed
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May add direct endocrine and reproductive toxicology with historical taxon identity retained. |
Structured after repository full-text and page-image review. The female hormone groups used triplicate beakers, but the separate reproductive comparison used one shared treatment beaker per group and only 0 to 8 of 20 females became ovigerous. Keep the pooled hormone samples, unclear beaker-to-assay mapping, nominal concentrations, unmatched alcohol positive-control vehicle, repeated paired tests and conditional brood denominators visible. Treat this as a third linked experiment, not an independent replication of the juvenile or male papers. |
Reproduction and population dynamics of the temperate freshwater shrimp Neocaridina denticulata denticulata in a Korean stream
2003; medium priority; method reviewed
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Could broaden field life-history context without turning a Korean stream population into aquarium timing. |
Structured after full-method review. Retain the five monthly box samples, length-frequency modeling, published taxon and field-to-aquarium boundary. |
Population ecology of Neocaridina denticulata in the Sugow River, Japan
1990; medium priority; method reviewed
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Adds an older field baseline for seasonality, density and population structure under a historical name. |
Structured after bilingual full-text review. Retain time-based netting, mesh selectivity, inferred cohorts and the allocated sex ratio for animals below 8 mm. |