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Controlled experiment Adjacent aquarium-system evidence

Nitrospira-Like Bacteria Associated with Nitrite Oxidation in Freshwater Aquaria

Hovanec et al., 1998. Applied and Environmental Microbiology 64(1): 258-264.

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This record is a claim boundary, not a quality badge. Evidence class and species relationship describe what was studied. They do not make the result universal, complete or automatically applicable to a home aquarium.

Source taxon as published
Freshwater aquarium nitrifying communities, not a Neocaridina animal experiment
Environment
Ammonium-dosed freshwater aquaria and aquarium biofilter samples
Life stages
not applicable
Reviewed
2026-08-12

What was studied

Study shape: Molecular probes, clone libraries and water chemistry followed nitrite oxidizers during aquarium establishment and an additive comparison.

Experimental unit: Aquarium for treatment and chemistry; sampled gravel or biofilter material for molecular endpoints

Endpoints: ammonia, nitrite, nitrate, rRNA sequence and probe signal.

Claim boundary

What it can support: Nitrospira-like organisms appeared with nitrite oxidation in the tested freshwater aquarium systems.

What it cannot support: One establishment design does not establish a universal cycle day, prove an additive effective or certify animal-ready capacity.

Publication status audit

Canonical DOI outside query result

The record has a canonical DOI but did not appear in the broad Neocaridina query. It requires DOI-specific or alternative-registry status review before any no-update statement.

Metadata snapshot reviewed 2026-08-12. Inspect the method, unmatched queue and limitations.

Correction impact

If this source boundary changes, these are the known downstream consumers. The list distinguishes scientific re-review from generated parity and historical follow-up.

Editorial review required (7)

This curated wording or synthesis must be reconsidered when the source boundary changes. A passing generator test cannot decide the new scientific meaning.

  • A tank is cycled after a fixed number of weeks Curated claim check
    The exact claim trace uses this record as interpretive context: Aquarium biofilters can contain ammonia- and nitrite-oxidizing communities that differ from older assumptions.
  • cycling decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • filtration decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • nitrogen decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • cycling practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • filtration practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • nitrogen practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.

Generated from governing registry (19)

This surface derives from a governing registry and should update with it, but release tests must still prove parity and routing.

Inspect the corpus-wide propagation audit.

How this record is classified

Controlled experiment: An assigned treatment or controlled comparison. The treatment, unit, duration and endpoint still limit the claim.

Adjacent aquarium-system evidence: The source studied aquarium chemistry, filtration, measurement or another engineered system rather than a Neocaridina animal endpoint.

Topics: Filtration, Nitrogen waste

Critically appraise this source without a score

These questions fit this record's controlled experiment role. They are prompts, not automatic judgments. Open the original source to answer them.

  1. Identity and provenance: How were the organism, population, stock and life stage identified and sourced? A published name or seller label can hide a taxonomic, population or life-stage transfer.
  2. Independent unit: What unit was independently assigned or sampled, and how many units support each comparison? Animal counts do not create independent replication when animals share a vessel, site, family or treatment history.
  3. Comparison and context: What was the actual comparator, allocation process, environment, duration and material condition? A result has meaning only against the comparison and conditions that produced it.
  4. Endpoint and measurement: Which endpoint was measured, with what method, unit, timing, resolution and decision rule? One endpoint cannot silently become survival, welfare, diagnosis, reproduction or long-term population performance.
  5. Denominators and missingness: Are starting counts, exclusions, losses, missing observations and analysis denominators reconciled? Unreported or changing denominators can alter the apparent direction, precision and applicability of a result.
  6. Transfer boundary: What is the nearest tempting aquarium claim that this source design cannot establish? Direct evidence can still be narrow, and adjacent evidence can be useful only while the inference remains visible.
  7. Treatment assignment: Were independent units assigned to treatments, and were baseline conditions comparable? Unclear allocation or baseline imbalance can confound the treatment comparison.
  8. Replication: Were treatment vessels or other exposure units independently replicated rather than subsampled? Repeated animals or assays inside one exposure unit do not replicate the environmental treatment.
  9. Exposure verification: Was the treatment, dose, feed intake or environmental exposure measured and maintained as described? Nominal treatment labels may not equal the exposure animals actually received.
  10. Analysis: Did the statistical model match the unit, repeated measures, multiplicity and missing outcomes? A precise p-value cannot repair a mismatched unit or unaccounted comparison structure.

Read the complete appraisal framework.

Where it is used

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