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Controlled experiment Direct Neocaridina evidence

Effects of dietary incorporation of Arthrospira (Spirulina) platensis meal on growth, survival, body composition, and reproductive performance of red cherry shrimp Neocaridina davidi (Crustacea, Atyidae) over successive spawnings

Namaei Kohal et al., 2018. Journal of Applied Phycology 30: 431-443.

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This record is a claim boundary, not a quality badge. Evidence class and species relationship describe what was studied. They do not make the result universal, complete or automatically applicable to a home aquarium.

Source taxon as published
Neocaridina davidi
Environment
Eighteen aerated aquaria at 26 to 28 C and pH 7.0 to 7.2 across an 11-month feeding trial, with complete water replacement every 10 days
Life stages
35-day-old juvenile, adult female, mature male, embryo, newly hatched juvenile
Reviewed
2026-08-12

What was studied

Study shape: Six formulated diets containing 0, 1, 3, 5, 8 or 10 percent Arthrospira meal were assigned to three aquaria each. Diets were approximately equal in protein and energy because Arthrospira replaced part of the fish meal; shrimp received 3 percent of wet biomass daily in three feedings and females were followed through eight spawning events.

Experimental unit: Aquarium, with three aquaria per diet; the independent incubation unit for detached eggs is not clearly described

Endpoints: weight and length growth, feed conversion ratio, survival, whole-body composition, time to maturity, inter-spawning interval, egg production, relative and actual fecundity, hatching percentage, hatchling length.

Claim boundary

What it can support: Under this exact formulated-diet protocol, several growth, female-survival and reproductive measures were higher at 8 or 10 percent Arthrospira meal than in the control, while the reported relative-fecundity advantage declined after the sixth spawning.

What it cannot support: The percentages describe dry-diet formulation, not an aquarium dose or addition to a finished food. Arthrospira replaced fish meal, control female survival was only 25.7 percent after 11 months, eggs were detached and artificially incubated, and incubation replication is unclear. The study does not establish a universal 8 to 10 percent recipe or validate a current commercial product.

Publication status audit

Matched in dated Crossref query

The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed.

Metadata snapshot reviewed 2026-08-12. Inspect the method, unmatched queue and limitations.

Correction impact

If this source boundary changes, these are the known downstream consumers. The list distinguishes scientific re-review from generated parity and historical follow-up.

Editorial review required (9)

This curated wording or synthesis must be reconsidered when the source boundary changes. A passing generator test cannot decide the new scientific meaning.

  • breeding decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • feeding decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • growth decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • breeding practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • feeding practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • growth practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • complete-diet-adequacy knowledge gap Canonical knowledge gap
    The current knowledge-gap boundary depends on a dated search that used this exact source and must be reconsidered if the source meaning changes.
  • complete-diet-adequacy answerability brief Answerability brief
    The decision, endpoint, minimum design or closure rule may change when evidence used in the linked gap search is corrected.
  • complete-diet-adequacy research allocation Current research allocation
    The current allocation, activation trigger, next permitted unit or stop decision may change when evidence used in the linked gap search is corrected.

Generated from governing registry (16)

This surface derives from a governing registry and should update with it, but release tests must still prove parity and routing.

Historical record needs follow-up decision (1)

Do not silently rewrite history. Decide whether the prior change record remains accurate and publish a new correction or scope note when needed.

Inspect the corpus-wide propagation audit.

How this record is classified

Controlled experiment: An assigned treatment or controlled comparison. The treatment, unit, duration and endpoint still limit the claim.

Direct Neocaridina evidence: The source measured animals named within Neocaridina. Direct still does not mean universal or sufficient.

Topics: Feeding, Growth and lifespan, Breeding

Critically appraise this source without a score

These questions fit this record's controlled experiment role. They are prompts, not automatic judgments. Open the original source to answer them.

  1. Identity and provenance: How were the organism, population, stock and life stage identified and sourced? A published name or seller label can hide a taxonomic, population or life-stage transfer.
  2. Independent unit: What unit was independently assigned or sampled, and how many units support each comparison? Animal counts do not create independent replication when animals share a vessel, site, family or treatment history.
  3. Comparison and context: What was the actual comparator, allocation process, environment, duration and material condition? A result has meaning only against the comparison and conditions that produced it.
  4. Endpoint and measurement: Which endpoint was measured, with what method, unit, timing, resolution and decision rule? One endpoint cannot silently become survival, welfare, diagnosis, reproduction or long-term population performance.
  5. Denominators and missingness: Are starting counts, exclusions, losses, missing observations and analysis denominators reconciled? Unreported or changing denominators can alter the apparent direction, precision and applicability of a result.
  6. Transfer boundary: What is the nearest tempting aquarium claim that this source design cannot establish? Direct evidence can still be narrow, and adjacent evidence can be useful only while the inference remains visible.
  7. Treatment assignment: Were independent units assigned to treatments, and were baseline conditions comparable? Unclear allocation or baseline imbalance can confound the treatment comparison.
  8. Replication: Were treatment vessels or other exposure units independently replicated rather than subsampled? Repeated animals or assays inside one exposure unit do not replicate the environmental treatment.
  9. Exposure verification: Was the treatment, dose, feed intake or environmental exposure measured and maintained as described? Nominal treatment labels may not equal the exposure animals actually received.
  10. Analysis: Did the statistical model match the unit, repeated measures, multiplicity and missing outcomes? A precise p-value cannot repair a mismatched unit or unaccounted comparison structure.

Read the complete appraisal framework.

Where it is used

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