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Controlled experiment Direct Neocaridina evidence

Promotive performance of shrimp Neocaridina denticulata on Typha angustifolia leaf litter decomposition

Kong et al., 2019. Hydrobiologia 827: 75-87.

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This record is a claim boundary, not a quality badge. Evidence class and species relationship describe what was studied. They do not make the result universal, complete or automatically applicable to a home aquarium.

Source taxon as published
River-collected Neocaridina denticulata, retained as published
Environment
Sixty-four aerated four-litre river-water microcosms in an environmental chamber set to 20 C, with measured water varying from 20 to 25 C during 140 days
Life stages
river-collected shrimp approximately 2.5 cm long
Reviewed
2026-08-12

What was studied

Study shape: Four treatments separated litter alone, direct shrimp contact with feces falling away, feces contact without direct shrimp contact, and shrimp plus litter plus feces together. Each shrimp unit held eight animals and 3 g of air-dried cattail litter. Four independently prepared buckets represented each treatment at each 35-day destructive harvest, for 64 buckets total. Filtered river water was replaced weekly.

Experimental unit: Four-litre bucket, with four independent buckets per treatment and harvest time; the paper describes destructive treatment-by-time units but reports date as a repeated factor without explaining how repeated units were linked

Endpoints: cumulative litter dry-mass loss, decomposition coefficient, interval mass loss, leaf-associated extracellular enzyme activities, pH, chemical oxygen demand, ammonium nitrogen, nitrate nitrogen, total nitrogen, total phosphorus.

Claim boundary

What it can support: After 140 days, cumulative litter mass loss was 51.7 percent in litter-only controls, 83.4 percent where shrimp contacted litter but feces fell away, 57.8 percent where feces contacted litter without shrimp contact, and 84.5 percent where shrimp, litter and feces remained together. The treatment contrasts support faster breakdown when shrimp had direct access under this microcosm protocol; water chemistry and enzyme responses also differed among treatments.

What it cannot support: Litter mass loss is not measured ingestion. Direct shrimp access combines grazing on leaf tissue or associated microbes, fragmentation and physical disturbance, while the reported grazing and feces shares were derived from treatment contrasts rather than measured intake. The experiment used one river population, one cattail preparation, eight shrimp in four litres and filtered river water replaced weekly, which the discussion says helped provide food; no formulated-food baseline was described. Reported zero mortality does not establish dietary adequacy because growth, condition, molting, reproduction and health were not measured. The paper switches between Typha angustifolia and Typha orientalis in treatment text and figure material, gives conflicting one-week versus one-to-two-day animal holding descriptions, does not report random allocation, lacks an obvious sham-mesh control, and does not reconcile destructive harvest with repeated-measures analysis. It also labels P values above 0.05 through 0.10 as biologically significant but noisy. The result does not recommend cattail, catappa or alder, establish a ration or replacement interval, prove a complete diet, or promise home-aquarium water safety.

Publication status audit

Matched in dated Crossref query

The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed.

Metadata snapshot reviewed 2026-08-12. Inspect the method, unmatched queue and limitations.

Correction impact

If this source boundary changes, these are the known downstream consumers. The list distinguishes scientific re-review from generated parity and historical follow-up.

Editorial review required (12)

This curated wording or synthesis must be reconsidered when the source boundary changes. A passing generator test cannot decide the new scientific meaning.

  • Neocaridina clean the tank Curated claim check
    The exact claim trace uses this record as limitation context: Separates litter breakdown and fecal effects from proven intake or whole-tank cleaning.
  • algae decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • feeding decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • habitat decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • Grazing on algae is not the same as cleaning an aquarium Curated evidence comparison
    The bounded reconciliation and unresolved question use this record in an explicit comparison.
  • algae practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • feeding practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • habitat practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • round1-synthesis-grazing Curated first-round reviewer assignment
    The frozen reviewer scope or consequential-failure check uses this record through the assigned claim trace or comparison and must be reconsidered before invitation or response.
  • botanical-litter-outcomes knowledge gap Canonical knowledge gap
    The current knowledge-gap boundary depends on a dated search that used this exact source and must be reconsidered if the source meaning changes.
  • botanical-litter-outcomes answerability brief Answerability brief
    The decision, endpoint, minimum design or closure rule may change when evidence used in the linked gap search is corrected.
  • botanical-litter-outcomes research allocation Current research allocation
    The current allocation, activation trigger, next permitted unit or stop decision may change when evidence used in the linked gap search is corrected.

Generated from governing registry (19)

This surface derives from a governing registry and should update with it, but release tests must still prove parity and routing.

Historical record needs follow-up decision (3)

Do not silently rewrite history. Decide whether the prior change record remains accurate and publish a new correction or scope note when needed.

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How this record is classified

Controlled experiment: An assigned treatment or controlled comparison. The treatment, unit, duration and endpoint still limit the claim.

Direct Neocaridina evidence: The source measured animals named within Neocaridina. Direct still does not mean universal or sufficient.

Topics: Feeding, Habitat, Nitrogen waste, Water parameters

Critically appraise this source without a score

These questions fit this record's controlled experiment role. They are prompts, not automatic judgments. Open the original source to answer them.

  1. Identity and provenance: How were the organism, population, stock and life stage identified and sourced? A published name or seller label can hide a taxonomic, population or life-stage transfer.
  2. Independent unit: What unit was independently assigned or sampled, and how many units support each comparison? Animal counts do not create independent replication when animals share a vessel, site, family or treatment history.
  3. Comparison and context: What was the actual comparator, allocation process, environment, duration and material condition? A result has meaning only against the comparison and conditions that produced it.
  4. Endpoint and measurement: Which endpoint was measured, with what method, unit, timing, resolution and decision rule? One endpoint cannot silently become survival, welfare, diagnosis, reproduction or long-term population performance.
  5. Denominators and missingness: Are starting counts, exclusions, losses, missing observations and analysis denominators reconciled? Unreported or changing denominators can alter the apparent direction, precision and applicability of a result.
  6. Transfer boundary: What is the nearest tempting aquarium claim that this source design cannot establish? Direct evidence can still be narrow, and adjacent evidence can be useful only while the inference remains visible.
  7. Treatment assignment: Were independent units assigned to treatments, and were baseline conditions comparable? Unclear allocation or baseline imbalance can confound the treatment comparison.
  8. Replication: Were treatment vessels or other exposure units independently replicated rather than subsampled? Repeated animals or assays inside one exposure unit do not replicate the environmental treatment.
  9. Exposure verification: Was the treatment, dose, feed intake or environmental exposure measured and maintained as described? Nominal treatment labels may not equal the exposure animals actually received.
  10. Analysis: Did the statistical model match the unit, repeated measures, multiplicity and missing outcomes? A precise p-value cannot repair a mismatched unit or unaccounted comparison structure.

Read the complete appraisal framework.

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