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Morphology or taxonomy Direct Neocaridina evidence

The occurrence process of chromatophores in three body color strains of the ornamental shrimp Neocaridina denticulata sinensis

Lu et al., 2022. Zoomorphology 141: 283-295.

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This record is a claim boundary, not a quality badge. Evidence class and species relationship describe what was studied. They do not make the result universal, complete or automatically applicable to a home aquarium.

Source taxon as published
Neocaridina denticulata sinensis
Environment
Red, yellow and blue laboratory strains cultured separately, with one strain cultured in a tank at 20 to 26 C and pH about 7.4; eggs were removed with a sodium chloride and trypsin solution, washed and cultured in a shaken 24-well plate before post-larvae were moved to a glass tank
Life stages
fertilized egg through membrane-zoea, newly hatched post-larva
Reviewed
2026-08-12

What was studied

Study shape: The authors photographed eight source-defined embryonic stages in red, yellow and blue strains, then followed chromatophore type, form, number and distribution into early post-hatch development. Six individuals per strain were used for the detailed chromatophore observations after pigment cells appeared, and six individuals per stage per strain were dissected from metanauplius through membrane-zoea.

Experimental unit: Individual embryo or post-larva for microscopy, with six individuals per strain for detailed observations after pigment cells appeared; the paper reports one culture tank per strain, does not report family contributions to the selected eggs and does not clearly describe independence across repeated photographs or inferential comparisons

Endpoints: chromatophore type, chromatophore form, chromatophore number, chromatophore diameter, chromatophore distribution, embryonic morphology.

Claim boundary

What it can support: Under this in-vitro protocol, no chromatophores were reported before pre-nauplius, erythrophores were the first visible chromatophore type in all three strains at the source-defined metanauplius stage, and later stage-specific observations included leucophores in red and yellow, cyanophores in blue and xanthophores in yellow after hatching.

What it cannot support: This does not establish a universal grading age, predict adult grade from an embryo or juvenile, validate a commercial colour-line identity or reveal inheritance. One tank per strain confounds strain with tank history, family contributions were unknown and only six individuals per strain were used for detailed observations. The egg-removal, trypsin, washing, shaker and in-vitro culture protocol is not a home breeding method. Food was not assigned as a treatment, adult colour was not followed, and discussion explanations involving carotenoids, yolk or feeding relied partly on hypotheses or unpublished data rather than a diet experiment. The paper gives one-way ANOVA but does not fully report the comparison plan, repeated-measure handling, assumption checks or exact P values, and its figure and text leave some post-hatch photographic timing ambiguous.

Publication status audit

Matched in dated Crossref query

The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed.

Metadata snapshot reviewed 2026-08-12. Inspect the method, unmatched queue and limitations.

Correction impact

If this source boundary changes, these are the known downstream consumers. The list distinguishes scientific re-review from generated parity and historical follow-up.

Editorial review required (6)

This curated wording or synthesis must be reconsidered when the source boundary changes. A passing generator test cannot decide the new scientific meaning.

  • Mixed colours always turn brown or clear in two generations Curated claim check
    The exact claim trace uses this record as interpretive context: Describes chromatophore development in three strains without predicting arbitrary named-line crosses.
  • colours decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • genetics decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • colours practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • genetics practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • round1-taxonomy-lineage Curated first-round reviewer assignment
    The frozen reviewer scope or consequential-failure check uses this record through the assigned claim trace or comparison and must be reconsidered before invitation or response.

Generated from governing registry (16)

This surface derives from a governing registry and should update with it, but release tests must still prove parity and routing.

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How this record is classified

Morphology or taxonomy: Anatomy, specimen identification or taxonomic relationships were studied with stated methods. A trade name or photograph cannot replace those methods.

Direct Neocaridina evidence: The source measured animals named within Neocaridina. Direct still does not mean universal or sufficient.

Topics: Colour, Anatomy, Breeding, Growth and lifespan, Genetics

Critically appraise this source without a score

These questions fit this record's morphology or taxonomy role. They are prompts, not automatic judgments. Open the original source to answer them.

  1. Identity and provenance: How were the organism, population, stock and life stage identified and sourced? A published name or seller label can hide a taxonomic, population or life-stage transfer.
  2. Independent unit: What unit was independently assigned or sampled, and how many units support each comparison? Animal counts do not create independent replication when animals share a vessel, site, family or treatment history.
  3. Comparison and context: What was the actual comparator, allocation process, environment, duration and material condition? A result has meaning only against the comparison and conditions that produced it.
  4. Endpoint and measurement: Which endpoint was measured, with what method, unit, timing, resolution and decision rule? One endpoint cannot silently become survival, welfare, diagnosis, reproduction or long-term population performance.
  5. Denominators and missingness: Are starting counts, exclusions, losses, missing observations and analysis denominators reconciled? Unreported or changing denominators can alter the apparent direction, precision and applicability of a result.
  6. Transfer boundary: What is the nearest tempting aquarium claim that this source design cannot establish? Direct evidence can still be narrow, and adjacent evidence can be useful only while the inference remains visible.
  7. Examined material: Which specimens, localities, sexes, stages, types or sequence accessions were actually examined? A diagnosis applies to examined material and methods, not automatically to a trade-line photograph.
  8. Diagnostic characters: Which characters or loci distinguish the taxon, and were alternatives explicitly compared? Color alone may be variable, convergent or unrelated to the diagnostic character.
  9. Name history: Were synonyms, historical combinations, type material and later revisions checked? An older title can describe a population that later work placed in another taxon.
  10. Consumer identification limit: Can the required character or method be observed reliably in an ordinary live-animal image? A valid taxonomic method may still be unavailable to a keeper examining one photograph.

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