Paradise under threat: the successful invasion of the freshwater shrimp Neocaridina davidi (Bouvier, 1904) and its microsporidian parasites on La Reunion Island

What was studied

Study shape: Forty shrimp were dip-netted at each of three sites and preserved immediately in 96 percent ethanol. All were identified morphologically, measured, sexed or classified as immature, dissected, visually screened internally and tested molecularly. Host CO1 and microsporidian SSU rRNA amplicons were Sanger sequenced and placed with reference sequences in maximum-likelihood phylogenies with 1000 bootstrap replicates.

Experimental unit: Individual field-collected shrimp for morphology, internal visual screening and molecular detection; one date and three sites in two catchments for the field occurrence observations

Endpoints: host morphology and CO1 identity, sex and life-stage counts, carapace length, microsporidian PCR-positive count, sequence similarity and phylogenetic placement, site occurrence, one-day temperature and conductivity.

Claim boundary

What it can support: Five of 120 sampled shrimp were PCR positive for four reported microsporidian sequence types: two Orthosomella lipae detections at the Salazie creek, and one Ecytonucleospora hepatopenaei, one Microsporidium sp. SRE01 and one Microsporidium sp. SRE02 detection at Bras Citronnier. None of the 40 Ravine Seche shrimp was positive. All 120 hosts shared one CO1 haplotype reported as 100 percent similar to the N. davidi reference set.

What it cannot support: This is a one-day field sample from three sites, not an aquarium or trade prevalence estimate. The paper reports no histology, tissue lesions, clinical signs, survival, experimental transmission, pathogenicity or treatment endpoint, and it did not test native co-occurring shrimp for parasites. Ethanol preservation prevented epibiont analysis. The single host CO1 haplotype cannot prove a single introduction, seller line, release event or movement route between catchments. Sequence similarity cannot establish parasite origin or that N. davidi introduced any organism. One Table 2 subgroup is internally inconsistent, listing 16 immatures while reporting two positives as 2 of 14, so subgroup percentages should not be repeated as settled denominators. The one-day site temperature and conductivity readings are not aquarium care limits, and zero of 40 at one site is not proof of absence.

Publication status audit

Matched in dated Crossref query

The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed.

Metadata snapshot reviewed 2026-08-12. Inspect the method, unmatched queue and limitations.

Correction impact

If this source boundary changes, these are the known downstream consumers. The list distinguishes scientific re-review from generated parity and historical follow-up.

Editorial review required (6)

This curated wording or synthesis must be reconsidered when the source boundary changes. A passing generator test cannot decide the new scientific meaning.

• health decision card Tank-side decision card
  The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
• quarantine decision card Tank-side decision card
  The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
• surplus decision card Tank-side decision card
  The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
• health practical guide Practical guide
  The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
• quarantine practical guide Practical guide
  The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
• surplus practical guide Practical guide
  The guide uses this evidence record and its practical wording must remain inside the revised source boundary.

Generated from governing registry (16)

This surface derives from a governing registry and should update with it, but release tests must still prove parity and routing.

• Record CSL-JSON citation Citation export
  The record citation export derives its note and source metadata from the evidence registry.
• Record RIS citation Citation export
  The record citation export derives its note and source metadata from the evidence registry.
• Complete CSL-JSON bibliography Citation export
  The complete citation export includes every evidence registry record.
• Complete RIS bibliography Citation export
  The complete citation export includes every evidence registry record.
• health portable decision card Generated decision-card text
  The portable text derives from the curated decision-card registry and must preserve every card section and both permanent links.
• quarantine portable decision card Generated decision-card text
  The portable text derives from the curated decision-card registry and must preserve every card section and both permanent links.
• surplus portable decision card Generated decision-card text
  The portable text derives from the curated decision-card registry and must preserve every card section and both permanent links.
• health complete visual decision card Generated decision-card visual
  The typography-only visual and exact accessibility description derive from the curated decision-card registry and must preserve every card section.
• quarantine complete visual decision card Generated decision-card visual
  The typography-only visual and exact accessibility description derive from the curated decision-card registry and must preserve every card section.
• surplus complete visual decision card Generated decision-card visual
  The typography-only visual and exact accessibility description derive from the curated decision-card registry and must preserve every card section.
• Paradise under threat: the successful invasion of the freshwater shrimp Neocaridina davidi (Bouvier, 1904) and its microsporidian parasites on La Reunion Island Canonical evidence page
  The canonical evidence page derives from the current evidence registry record.
• health evidence dossier Generated guide dossier
  The dossier derives its linked evidence set from the record care path.
• quarantine evidence dossier Generated guide dossier
  The dossier derives its linked evidence set from the record care path.
• surplus evidence dossier Generated guide dossier
  The dossier derives its linked evidence set from the record care path.
• Machine-readable knowledge export Machine-readable knowledge export
  The versioned export includes the complete evidence registry and source-integrity audit.
• Research map record Research map
  The research map derives its source boundary from the evidence registry.

Historical record needs follow-up decision (1)

Do not silently rewrite history. Decide whether the prior change record remains accurate and publish a new correction or scope note when needed.

• Five molecular detections do not reveal an introduction route Historical research change
  The historical change cites this source. Preserve the old decision and decide whether a new public correction is required.

Inspect the corpus-wide propagation audit.