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Controlled experiment Direct Neocaridina evidence

Body coloration, carotenoid content, spermatophore quality and biochemical parameters by effect of temperature

Tomas et al., 2021. Acta Zoologica 102(3): 297-309.

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This record is a claim boundary, not a quality badge. Evidence class and species relationship describe what was studied. They do not make the result universal, complete or automatically applicable to a home aquarium.

Source taxon as published
Neocaridina davidi
Environment
Laboratory aquaria at 20, 24 and 28 C
Life stages
adult male, adult female
Reviewed
2026-08-12

What was studied

Study shape: Reproductive, pigmentation and biochemical outcomes were compared across three assigned temperatures.

Experimental unit: Temperature treatment aquarium

Endpoints: survival, ovarian maturation, ovigerous females, weight, colour, spermatophore quality.

Claim boundary

What it can support: Lower temperature delayed ovarian maturation in this protocol while survival remained above 90 percent in every treatment.

What it cannot support: It does not make every temperature in the tested range equivalent or establish chronic survival outside the study duration.

Publication status audit

Matched in dated Crossref query

The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed.

Metadata snapshot reviewed 2026-08-12. Inspect the method, unmatched queue and limitations.

Correction impact

If this source boundary changes, these are the known downstream consumers. The list distinguishes scientific re-review from generated parity and historical follow-up.

Editorial review required (9)

This curated wording or synthesis must be reconsidered when the source boundary changes. A passing generator test cannot decide the new scientific meaning.

  • Warmer water always makes shrimp grow and breed better Curated claim check
    The exact claim trace uses this record as direct support: Measures color, biochemical and spermatophore endpoints across tested temperatures rather than universal welfare.
  • breeding decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • colours decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • temperature decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • Temperature results depend on the outcome being measured Curated evidence comparison
    The bounded reconciliation and unresolved question use this record in an explicit comparison.
  • breeding practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • colours practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • temperature practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • round1-design-temperature Curated first-round reviewer assignment
    The frozen reviewer scope or consequential-failure check uses this record through the assigned claim trace or comparison and must be reconsidered before invitation or response.

Generated from governing registry (19)

This surface derives from a governing registry and should update with it, but release tests must still prove parity and routing.

Inspect the corpus-wide propagation audit.

How this record is classified

Controlled experiment: An assigned treatment or controlled comparison. The treatment, unit, duration and endpoint still limit the claim.

Direct Neocaridina evidence: The source measured animals named within Neocaridina. Direct still does not mean universal or sufficient.

Topics: Temperature, Breeding, Colour

Critically appraise this source without a score

These questions fit this record's controlled experiment role. They are prompts, not automatic judgments. Open the original source to answer them.

  1. Identity and provenance: How were the organism, population, stock and life stage identified and sourced? A published name or seller label can hide a taxonomic, population or life-stage transfer.
  2. Independent unit: What unit was independently assigned or sampled, and how many units support each comparison? Animal counts do not create independent replication when animals share a vessel, site, family or treatment history.
  3. Comparison and context: What was the actual comparator, allocation process, environment, duration and material condition? A result has meaning only against the comparison and conditions that produced it.
  4. Endpoint and measurement: Which endpoint was measured, with what method, unit, timing, resolution and decision rule? One endpoint cannot silently become survival, welfare, diagnosis, reproduction or long-term population performance.
  5. Denominators and missingness: Are starting counts, exclusions, losses, missing observations and analysis denominators reconciled? Unreported or changing denominators can alter the apparent direction, precision and applicability of a result.
  6. Transfer boundary: What is the nearest tempting aquarium claim that this source design cannot establish? Direct evidence can still be narrow, and adjacent evidence can be useful only while the inference remains visible.
  7. Treatment assignment: Were independent units assigned to treatments, and were baseline conditions comparable? Unclear allocation or baseline imbalance can confound the treatment comparison.
  8. Replication: Were treatment vessels or other exposure units independently replicated rather than subsampled? Repeated animals or assays inside one exposure unit do not replicate the environmental treatment.
  9. Exposure verification: Was the treatment, dose, feed intake or environmental exposure measured and maintained as described? Nominal treatment labels may not equal the exposure animals actually received.
  10. Analysis: Did the statistical model match the unit, repeated measures, multiplicity and missing outcomes? A precise p-value cannot repair a mismatched unit or unaccounted comparison structure.

Read the complete appraisal framework.

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