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Controlled experiment Direct Neocaridina evidence

Shelter preference and variation in the daily activity pattern of the ornamental shrimp Neocaridina davidi

Carvalho-Batista et al., 2023. Nauplius 31: e2023018.

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This record is a claim boundary, not a quality badge. Evidence class and species relationship describe what was studied. They do not make the result universal, complete or automatically applicable to a home aquarium.

Source taxon as published
Neocaridina davidi
Environment
Fifteen 23-litre laboratory aquarium groups at 23 C under a 12-hour light and 12-hour dark cycle, with red incandescent light used for night observations and no feeding during the 72-hour experiment
Life stages
male with 3.04 to 3.68 mm carapace length, non-ovigerous female with 4.74 to 6.39 mm carapace length, ovigerous female with 5.1 to 6.39 mm carapace length
Reviewed
2026-08-12

What was studied

Study shape: Ten shrimp from one sex or reproductive class shared an aquarium offering Java moss, wood, Egeria and rock shelters of about 250 square centimetres each. Shelter positions were varied, and the same group was counted repeatedly during eight 20-minute periods per day for three days.

Experimental unit: Ten-shrimp aquarium group, with five consecutive groups per sex or reproductive class and repeated observations of the same animals across 72 hours

Endpoints: overall shelter occupancy, occupancy by shelter type, day and night shelter use, walking and swimming frequency.

Claim boundary

What it can support: Across the tested groups, mean shelter use was 82.1 percent per observation, shelter use was higher by day than night, and Java moss was used most overall, followed by wood, while rock was used least.

What it cannot support: Shelter material, shape and structural complexity changed together, so the comparison does not isolate a live-plant, moss-species or complexity effect. The paper does not state a repeated-measures or aquarium-level model for the many observations of the same groups. Animals were not fed during the 72 hours, night observations used a red lamp, and no no-shelter, predator, stress, survival, growth or reproduction outcome was tested. The result does not make one plant required, define an ideal hidden percentage, diagnose stress or prove protection from fish.

Publication status audit

Matched in dated Crossref query

The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed.

Metadata snapshot reviewed 2026-08-12. Inspect the method, unmatched queue and limitations.

Correction impact

If this source boundary changes, these are the known downstream consumers. The list distinguishes scientific re-review from generated parity and historical follow-up.

Editorial review required (11)

This curated wording or synthesis must be reconsidered when the source boundary changes. A passing generator test cannot decide the new scientific meaning.

  • Peaceful fish are shrimp-safe Curated claim check
    The exact claim trace uses this record as interpretive context: Shelter and context affect shrimp responses but the study does not validate a fish-safe list.
  • behaviour decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • habitat decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • tankmates decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • behaviour practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • habitat practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • tankmates practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • round1-husbandry-tankmates Curated first-round reviewer assignment
    The frozen reviewer scope or consequential-failure check uses this record through the assigned claim trace or comparison and must be reconsidered before invitation or response.
  • tankmate-recruitment knowledge gap Canonical knowledge gap
    The current knowledge-gap boundary depends on a dated search that used this exact source and must be reconsidered if the source meaning changes.
  • tankmate-recruitment answerability brief Answerability brief
    The decision, endpoint, minimum design or closure rule may change when evidence used in the linked gap search is corrected.
  • tankmate-recruitment research allocation Current research allocation
    The current allocation, activation trigger, next permitted unit or stop decision may change when evidence used in the linked gap search is corrected.

Generated from governing registry (19)

This surface derives from a governing registry and should update with it, but release tests must still prove parity and routing.

Historical record needs follow-up decision (1)

Do not silently rewrite history. Decide whether the prior change record remains accurate and publish a new correction or scope note when needed.

Inspect the corpus-wide propagation audit.

How this record is classified

Controlled experiment: An assigned treatment or controlled comparison. The treatment, unit, duration and endpoint still limit the claim.

Direct Neocaridina evidence: The source measured animals named within Neocaridina. Direct still does not mean universal or sufficient.

Topics: Behaviour, Habitat

Critically appraise this source without a score

These questions fit this record's controlled experiment role. They are prompts, not automatic judgments. Open the original source to answer them.

  1. Identity and provenance: How were the organism, population, stock and life stage identified and sourced? A published name or seller label can hide a taxonomic, population or life-stage transfer.
  2. Independent unit: What unit was independently assigned or sampled, and how many units support each comparison? Animal counts do not create independent replication when animals share a vessel, site, family or treatment history.
  3. Comparison and context: What was the actual comparator, allocation process, environment, duration and material condition? A result has meaning only against the comparison and conditions that produced it.
  4. Endpoint and measurement: Which endpoint was measured, with what method, unit, timing, resolution and decision rule? One endpoint cannot silently become survival, welfare, diagnosis, reproduction or long-term population performance.
  5. Denominators and missingness: Are starting counts, exclusions, losses, missing observations and analysis denominators reconciled? Unreported or changing denominators can alter the apparent direction, precision and applicability of a result.
  6. Transfer boundary: What is the nearest tempting aquarium claim that this source design cannot establish? Direct evidence can still be narrow, and adjacent evidence can be useful only while the inference remains visible.
  7. Treatment assignment: Were independent units assigned to treatments, and were baseline conditions comparable? Unclear allocation or baseline imbalance can confound the treatment comparison.
  8. Replication: Were treatment vessels or other exposure units independently replicated rather than subsampled? Repeated animals or assays inside one exposure unit do not replicate the environmental treatment.
  9. Exposure verification: Was the treatment, dose, feed intake or environmental exposure measured and maintained as described? Nominal treatment labels may not equal the exposure animals actually received.
  10. Analysis: Did the statistical model match the unit, repeated measures, multiplicity and missing outcomes? A precise p-value cannot repair a mismatched unit or unaccounted comparison structure.

Read the complete appraisal framework.

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