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Controlled experiment Direct Neocaridina evidence

Toxic effects of nickel on tolerance and regeneration in the freshwater shrimp Neocaridina davidi

Ostróżka et al., 2024. The European Zoological Journal.

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This record is a claim boundary, not a quality badge. Evidence class and species relationship describe what was studied. They do not make the result universal, complete or automatically applicable to a home aquarium.

Source taxon as published
Neocaridina davidi
Environment
Adult shrimp exposed individually to nickel chloride for one or two weeks, followed by one or two weeks in clean water at 21 C, pH 7 and GH 10
Life stages
adult
Reviewed
2026-08-12

What was studied

Study shape: The authors first screened nominal 0.09, 0.9 and 3.63 mg Ni/L, selected 3.63 mg Ni/L after observing about 50 percent mortality at 14 days, then compared one- and two-week exposures and one- or two-week clean-water periods. Whole-body nickel, midgut structure, reserves, reactive-oxygen and cell-death measures were assessed. The linked CC BY deposit supplies quantitative tables and microscopy files.

Experimental unit: Individually held adult for exposure, with animal, whole-body preparation or isolated organ used according to endpoint. The deposited TXRF table has three numbered samples per group, while reactive-oxygen tables contain uneven row counts and repeated analysis dates that do not identify every row as an independent animal.

Endpoints: preliminary mortality, whole-body nickel, intestinal degeneration, hepatopancreatic change, reserve material, reactive oxygen species, autophagic signals, apoptosis and necrosis, clean-water response.

Claim boundary

What it can support: At the selected high nominal nickel treatment, adults accumulated nickel and showed organ- and time-associated cellular damage; some measured changes persisted after one or two weeks in clean water.

What it cannot support: The approximately 50 percent 14-day mortality was a preliminary selection observation, not a reported formal LC50 estimate with uncertainty. The deposit measures nickel in whole-shrimp samples, not the exposure water, so 3.63 mg Ni/L remains nominal; the recovery groups necessarily contained survivors, controls remained in the continuous culture and the repository does not resolve whether every reactive-oxygen row is a biological unit rather than a repeat or batch observation. It does not establish whole-animal recovery, a household threshold, a copper or fertilizer limit, or a diagnosis from appearance.

Publication status audit

Matched in dated Crossref query

The canonical DOI appeared in the dated broad query and carried no registered update in that response. This is a metadata observation, not proof that the work has never changed.

Metadata snapshot reviewed 2026-08-12. Inspect the method, unmatched queue and limitations.

Correction impact

If this source boundary changes, these are the known downstream consumers. The list distinguishes scientific re-review from generated parity and historical follow-up.

Editorial review required (6)

This curated wording or synthesis must be reconsidered when the source boundary changes. A passing generator test cannot decide the new scientific meaning.

  • anatomy decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • chemical-safety decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • health decision card Tank-side decision card
    The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
  • anatomy practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • chemical-safety practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
  • health practical guide Practical guide
    The guide uses this evidence record and its practical wording must remain inside the revised source boundary.

Generated from governing registry (16)

This surface derives from a governing registry and should update with it, but release tests must still prove parity and routing.

Historical record needs follow-up decision (1)

Do not silently rewrite history. Decide whether the prior change record remains accurate and publish a new correction or scope note when needed.

Inspect the corpus-wide propagation audit.

How this record is classified

Controlled experiment: An assigned treatment or controlled comparison. The treatment, unit, duration and endpoint still limit the claim.

Direct Neocaridina evidence: The source measured animals named within Neocaridina. Direct still does not mean universal or sufficient.

Topics: Chemical exposure, Anatomy, Health

Critically appraise this source without a score

These questions fit this record's controlled experiment role. They are prompts, not automatic judgments. Open the original source to answer them.

  1. Identity and provenance: How were the organism, population, stock and life stage identified and sourced? A published name or seller label can hide a taxonomic, population or life-stage transfer.
  2. Independent unit: What unit was independently assigned or sampled, and how many units support each comparison? Animal counts do not create independent replication when animals share a vessel, site, family or treatment history.
  3. Comparison and context: What was the actual comparator, allocation process, environment, duration and material condition? A result has meaning only against the comparison and conditions that produced it.
  4. Endpoint and measurement: Which endpoint was measured, with what method, unit, timing, resolution and decision rule? One endpoint cannot silently become survival, welfare, diagnosis, reproduction or long-term population performance.
  5. Denominators and missingness: Are starting counts, exclusions, losses, missing observations and analysis denominators reconciled? Unreported or changing denominators can alter the apparent direction, precision and applicability of a result.
  6. Transfer boundary: What is the nearest tempting aquarium claim that this source design cannot establish? Direct evidence can still be narrow, and adjacent evidence can be useful only while the inference remains visible.
  7. Treatment assignment: Were independent units assigned to treatments, and were baseline conditions comparable? Unclear allocation or baseline imbalance can confound the treatment comparison.
  8. Replication: Were treatment vessels or other exposure units independently replicated rather than subsampled? Repeated animals or assays inside one exposure unit do not replicate the environmental treatment.
  9. Exposure verification: Was the treatment, dose, feed intake or environmental exposure measured and maintained as described? Nominal treatment labels may not equal the exposure animals actually received.
  10. Analysis: Did the statistical model match the unit, repeated measures, multiplicity and missing outcomes? A precise p-value cannot repair a mismatched unit or unaccounted comparison structure.

Read the complete appraisal framework.

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Where it is used

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