Autophagy and apoptosis in starved and re-fed Neocaridina davidi (Crustacea, Malacostraca) midgut

What was studied

Study shape: Adults were deprived of feed for 7, 14 or 21 days, and subsets were re-fed for 4, 7 or 14 days. TEM, TUNEL, LysoTracker and caspase-3 methods assessed intestine and hepatopancreas cells. Non-starved reference values and images were imported from a 2016 paper rather than produced as a concurrent control group.

Experimental unit: Shrimp were isolated individually, but Table 1 lists 10 TEM animals and 8 animals for each confocal method in most groups while quantitative Tables 2 and 3 report n = 5; animal reuse, pooling and the cell-to-animal analysis hierarchy are not resolved

Endpoints: midgut cell ultrastructure, autophagosome-bearing cell percentage, TUNEL-labelled cell percentage, qualitative LysoTracker signal, qualitative caspase-3 signal, unquantified mortality during 21-day deprivation.

Claim boundary

What it can support: Within this linked laboratory series, 7 and 14 days without feed were associated with more reported autophagosome-bearing midgut cells, and tissue descriptions after 14 days included fewer organelles and less reserve material. After re-feeding, qualitative tissue structure and the reported cellular markers moved toward the earlier non-starved reference.

What it cannot support: This paper reused the prior experimental procedure and imported its non-starved controls, so it is not independent replication and does not provide a concurrent baseline. Most animals deprived for 21 days reportedly died, but mortality counts, timing and survival analysis were absent; the paper says that group was not quantitatively analyzed even though Table 1 lists post-21-day TEM specimens. Re-feeding food amount, frequency and intake were not reported. Table 1 animal counts conflict with the n = 5 quantitative tables, the nested cell and animal hierarchy is unclear, random allocation and blinding were not reported, and the t tests omit comparison pairs, assumptions, exact p values and multiplicity handling. Table 3 prints zero means with positive standard deviations for nonnegative percentages, which is internally impossible. Caspase-3 and LysoTracker results were qualitative, marker validation was limited, and the TUNEL threshold was described as arbitrary. The study measured no growth, molting, behaviour, reproduction, longevity or whole-animal recovery and cannot establish a routine fast, safe missed-feeding interval, re-feeding recipe, home diagnosis or health benefit.

Publication status audit

Matched in dated Crossref query

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Metadata snapshot reviewed 2026-08-12. Inspect the method, unmatched queue and limitations.

Correction impact

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Editorial review required (6)

This curated wording or synthesis must be reconsidered when the source boundary changes. A passing generator test cannot decide the new scientific meaning.

• anatomy decision card Tank-side decision card
  The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
• feeding decision card Tank-side decision card
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• health decision card Tank-side decision card
  The card translates this guide into an observation, first action, next record and stopping boundary, so all four require editorial review when the source changes.
• anatomy practical guide Practical guide
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• feeding practical guide Practical guide
  The guide uses this evidence record and its practical wording must remain inside the revised source boundary.
• health practical guide Practical guide
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Generated from governing registry (16)

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• anatomy portable decision card Generated decision-card text
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• feeding portable decision card Generated decision-card text
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• health portable decision card Generated decision-card text
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• anatomy complete visual decision card Generated decision-card visual
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• Autophagy and apoptosis in starved and re-fed Neocaridina davidi (Crustacea, Malacostraca) midgut Canonical evidence page
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• anatomy evidence dossier Generated guide dossier
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• feeding evidence dossier Generated guide dossier
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• health evidence dossier Generated guide dossier
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Historical record needs follow-up decision (1)

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• Cellular recovery is not a re-feeding recipe Historical research change
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